Red light accelerates the formation of a human dermal equivalent

Oliveira, Anna Cb; Morais, Thayz Fl; Bernal, Cláudia; Martins, Virgínia da Conceição Amaro; Plepis, Ana Maria de Guzzi; Menezes, Priscila Fc; Perussi, Janice Rodrigues

doi:10.1177/0885328218759385

Cited by 5 publications

(3 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Non-ionising electromagnetic radiation (EMR) in the visible light spectrum has been used as a therapeutic modality to improve wound and soft tissue healing, reduce the appearance of aging, increase the rate of hair growth, reduce pain and inflammation, and has potential as an alternative cancer therapy [6][7][8][9][10][11] . More recently, light has demonstrated utility during in vitro cell and tissue culture, acting as an antioxidant substitute post-thawing of human stem cells and accelerating the formation of a dermal equivalent for human skin modelling 12,13 . Previously referred to as low-level laser therapy (LLLT), the use of light in biology is now more accurately referred to as photobiomodulation (PBM).…”

mentioning

confidence: 99%

Investigating the effects of low intensity visible light on human keratinocytes using a customized LED exposure system

Sutterby

Chheang

Thurgood

et al. 2022

Sci Rep

View full text Add to dashboard Cite

Photobiomodulation (PBM) refers to the use of light to modulate cellular processes, and has demonstrated utility in improving wound healing outcomes, and reducing pain and inflammation. Despite the potential benefits of PBM, the precise molecular mechanisms through which it influences cell behavior are not yet well understood. Inconsistent reporting of key light parameters has created uncertainty around optimal exposure profiles. In addition, very low intensities of light, < 0.1 J/cm2, have not been thoroughly examined for their use in PBM. Here, we present a custom-made compact, and modular LED-based exposure system for studying the effects of very low-intensity visible light (cell proliferation, migration, ROS production, and mitochondrial membrane potential) of three different wavelengths in a parallel manner. The device allows for six repeats of three different exposure conditions plus a non-irradiated control on a single 24-well plate. The immortalised human keratinocyte cell line, HaCaT, was selected as a major cellular component of the skin epidermal barrier. Furthermore, an in vitro wound model was developed by allowing the HaCaT to form a confluent monolayer, then scratching the cells with a pipette tip to form a wound. Cells were exposed to yellow (585 nm, 0.09 mW, ~ 3.7 mJ/cm2), orange (610 nm, 0.8 mW, ~ 31 mJ/cm2), and red (660 nm, 0.8 mW, ~ 31 mJ/cm2) light for 10 min. 48 h post-irradiation, immunohistochemistry was performed to evaluate cell viability, proliferation, ROS production, and mitochondrial membrane potential. The results demonstrate increased proliferation and decreased scratch area for all exposure conditions, however only red light increased the mitochondrial activity. Oxidative stress levels did not increase for any of the exposures. The present exposure system provides opportunities to better understand the complex cellular mechanisms driven by the irradiation of skin cells with visible light.

show abstract

mentioning

confidence: 99%

Investigating the effects of low intensity visible light on human keratinocytes using a customized LED exposure system

Sutterby

Chheang

Thurgood

et al. 2022

Sci Rep

View full text Add to dashboard Cite

show abstract

“…The amount of glass transition temperature was obtained by the curve inflection. In this work, T g was expressed as the midpoint of the step 45,46 …”

Section: Methodsmentioning

confidence: 99%

Mechanical and biological performance of rainbow trout collagen‐boron nitride nanocomposite scaffolds for soft tissue engineering

Najafi

Kharaziha

Karimzadeh

et al. 2021

J of Applied Polymer Sci

View full text Add to dashboard Cite

We aim to investigate the potential of collagen extracted from rainbow trout for tissue engineering applications. In this regard, nanocomposite scaffolds based on the extracted collagen reinforced with various concentrations of boron nitride (BN) nanoparticles (0, 3, 6, 9, and 12 wt%) were developed. In addition, the role of various concentrations of BN nanoparticles and two‐step cross‐linking process on the physical and chemical properties of nanocomposite scaffolds were investigated. Our results demonstrated the isolation of Type I collagen with excellent thermal stability but with some structural and chemical differences compared to other sources. The synergic role of BN nanoparticles and two‐step cross‐linking process resulted in a noticeable improvement in the mechanical properties of collagen‐BN scaffolds. Noticeably, incorporation of 6 wt% BN along with a two‐step cross‐linking process significantly increased the compressive strength (9.5 times) and elastic modulus (four times) of the collagen scaffold. Besides, nanocomposite scaffolds significantly improved proliferation and spreading of MG‐63 cell line, confirming their biocompatibility. The results suggested that the incorporation of BN nanoparticles along with a two‐step cross‐linking process not only could promote the mechanical and thermal performances of collagen scaffolds, but also enhanced high cell viability, and proliferation supporting their potential in tissue engineering applications.

show abstract

“… 101 It seems that PBM activates cell division in sufficiently soft scaffold systems, such as decellularized dermal matrices and hydrogels. 160 – 162 …”

Section: Pbm For Tissue Engineering and Regenerative Medicinementioning

confidence: 99%

Photobiomodulation in 3D tissue engineering

et al. 2022

View full text Add to dashboard Cite

. Significance : The method of photobiomodulation (PBM) has been used in medicine for a long time to promote anti-inflammation and pain-resolving processes in different organs and tissues. PBM triggers numerous cellular pathways including stimulation of the mitochondrial respiratory chain, alteration of the cytoskeleton, cell death prevention, increasing proliferative activity, and directing cell differentiation. The most effective wavelengths for PBM are found within the optical window (750 to 1100 nm), in which light can permeate tissues and other water-containing structures to depths of up to a few cm. PBM already finds its applications in the developing fields of tissue engineering and regenerative medicine. However, the diversity of three-dimensional (3D) systems, irradiation sources, and protocols intricate the PBM applications. Aim: We aim to discuss the PBM and 3D tissue engineered constructs to define the fields of interest for PBM applications in tissue engineering. Approach : First, we provide a brief overview of PBM and the timeline of its development. Then, we discuss the optical properties of 3D cultivation systems and important points of light dosimetry. Finally, we analyze the cellular pathways induced by PBM and outcomes observed in various 3D tissue-engineered constructs: hydrogels, scaffolds, spheroids, cell sheets, bioprinted structures, and organoids. Results: Our summarized results demonstrate the great potential of PBM in the stimulation of the cell survival and viability in 3D conditions. The strategies to achieve different cell physiology states with particular PBM parameters are outlined. Conclusions: PBM has already proved itself as a convenient and effective tool to prevent drastic cellular events in the stress conditions. Because of the poor viability of cells in scaffolds and the convenience of PBM devices, 3D tissue engineering is a perspective field for PBM applications.

show abstract

Red light accelerates the formation of a human dermal equivalent

Cited by 5 publications

References 53 publications

Investigating the effects of low intensity visible light on human keratinocytes using a customized LED exposure system

Investigating the effects of low intensity visible light on human keratinocytes using a customized LED exposure system

Mechanical and biological performance of rainbow trout collagen‐boron nitride nanocomposite scaffolds for soft tissue engineering

Photobiomodulation in 3D tissue engineering

Contact Info

Product

Resources

About