BMP-2 induces angiogenesis by provoking integrin α6 expression in human endothelial progenitor cells

Chen, Wei-Cheng; Chung, Ching‐Hu; Lu, Yung‐Chang; Wu, Min-Huan; Chou, Po-Hsun; Yen, Juei-Yu; Lai, Yu-Wei; Wang, Guo-Shou; Liu, Shih‐Chia; Cheng, Jen-Kun; Wu, Yih‐Jer; Yeh, Hung‐I; Wang, Li-Yu; Wang, Shih‐Wei

doi:10.1016/j.bcp.2018.02.021

Cited by 37 publications

(38 citation statements)

References 49 publications

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“…As previously reported, LIPUS enhances angiogenesis by increasing VEGF after acute myocardial infarction (39). Additionally, BMP-2 treatment stimulated angiogenesis in human endothelial progenitor cells (40). The incorporation of either BMP-2 or VEGF inside porous scaffolds has been demonstrated to induce both angiogenesis and osteogenesis in a previous study (41).…”

Section: Discussionsupporting

confidence: 54%

The combination of PLLA/PLGA/PCL composite scaffolds integrated with BMP‑2‑loaded microspheres and low‑intensity pulsed ultrasound alleviates steroid‑induced osteonecrosis of the femoral head

et al. 2020

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Section: Discussionsupporting

confidence: 54%

The combination of PLLA/PLGA/PCL composite scaffolds integrated with BMP‑2‑loaded microspheres and low‑intensity pulsed ultrasound alleviates steroid‑induced osteonecrosis of the femoral head

et al. 2020

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“…Interestingly, it was reported that RUNX-2 is a component of the genetic program that modulate the expression of VEGF during endochondral bone formation (Zelzer et al, 2001). Furthermore, BMP-2 was found to be able to promote in vitro angiogenesis in human endothelial progenitor cells (Chen et al, 2018) and BMP-2 treatment enhanced VEGFA expression in adipose stem cells grown on biphasic calcium phosphate (Overman et al, 2013). In addition, BMP-4 increased the secretion of VEGF in adult retinal pigment epithelium-19 and in osteoblast-like MC3T3-E1 cells (Kozawa et al, 2001; Vogt et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Engineered Extracellular Vesicles From Human Periodontal-Ligament Stem Cells Increase VEGF/VEGFR2 Expression During Bone Regeneration

et al. 2019

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Bone regeneration represents still a challenge, in particular for calvarium defects. Recently, the development of biomaterials with the addiction of stem cells is giving promising results for the treatment of bone defects. In particular, it was demonstrated that scaffolds enriched with mesenchymal stem cells (MSCs) and/or their derivatives, such as conditioned medium (CM) and extracellular vesicles (EVs), may improve bone regeneration. Moreover, given the deep link between osteogenesis and angiogenesis, a successful approach must also take into consideration the development of vascularization. In this work we evaluated the bone regeneration capacity of a collagen membrane (3D-COL) enriched with human periodontal-ligament stem cells (hPDLSCs) and CM or EVs or EVs engineered with polyethylenimine (PEI-EVs) in rats subjected to a calvarial defect. We evaluated also their capacity to induce angiogenic factors. At first, in vitro results showed an increased expression of osteogenic markers in hPDLSCs cultured with the 3D-COL and PEI-EVs, associated also with the increased protein levels of Vascular endothelial growth factor (VEGF) and VEGF receptor 2 (VEGFR2). The increased expression of these proteins was confirmed also in vivo in rats implanted with the 3D-COL enriched with hPDLSCs and PEI-EVs. Moreover, histological examination evidenced in this group of rats the activation of bone regeneration and of the vascularization process. Also MicroCT imaging with morphometric analysis confirmed in rats transplanted with 3D-COL enriched with hPDLSCs and PEI-EVs an important regenerative process and a better integration level. All together, these results evidenced that the 3D-COL enriched with hPDLSCs and PEI-EVs may promote bone regeneration of calvaria defects, associated also with an increased vascularization.

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“…Therefore, chondromodulin secreted from the cartilage tissue filling the holes in 75TCP inhibited angiogenesis and preserved cartilage tissue in 75TCP. In addition, since BMP-2 has been reported to be involved in cell aggregation and angiogenesis26,27, if the concentration of BMP-2 is low, so is the capacity for bone formation induced by angiogenesis. Therefore, 75TCP with a low concentration of BMP-2 is advantageous for cartilage formation.…”

Section: Discussionmentioning

confidence: 99%

Effects of the Geometrical Structure of a Honeycomb TCP on Relationship between Bone / Cartilage Formation and Angiogenesis

et al. 2018

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A number of biomaterials have been developed, some of which already enjoy widespread clinic use. We have devised a new honeycomb tricalcium phosphate (TCP) containing through-and-through holes of various diameters to control cartilage and bone formation. However, the way in which the geometric structure of the honeycomb TCP controls cartilage and bone tissue formation separately remains unknown. In addition, an association has been reported between bone formation and angiogenesis. Therefore, in the present study, we investigated the relationship between angiogenesis and various hole diameters in our honeycomb TCP over time in a rat ectopic hard tissue formation model. Honeycomb TCPs with hole diameters of 75, 300, and 500 μm were implanted into rat femoral muscle. Next, ectopic hard tissue formation in the holes of the honeycomb TCP was assessed histologically at postoperative weeks 1, 2, and 3, and CD34 immunostaining was performed to evaluate angiogenesis. The results showed that cartilage formation accompanied by thin and poor blood vessel formation, bone marrow-like tissue with a branching network of vessels, and vigorous bone formation with thick linear blood vessels occurred in the TCPs with 75-μm, 300-μm, and 500-μm hole diameters, respectively. These results indicated that the geometrical structure of the honeycomb TCP affected cartilage and bone tissue formation separately owing to the induced angiogenesis and altered oxygen partial pressure within the holes.

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BMP-2 induces angiogenesis by provoking integrin α6 expression in human endothelial progenitor cells

Cited by 37 publications

References 49 publications

The combination of PLLA/PLGA/PCL composite scaffolds integrated with BMP‑2‑loaded microspheres and low‑intensity pulsed ultrasound alleviates steroid‑induced osteonecrosis of the femoral head

The combination of PLLA/PLGA/PCL composite scaffolds integrated with BMP‑2‑loaded microspheres and low‑intensity pulsed ultrasound alleviates steroid‑induced osteonecrosis of the femoral head

Engineered Extracellular Vesicles From Human Periodontal-Ligament Stem Cells Increase VEGF/VEGFR2 Expression During Bone Regeneration

Effects of the Geometrical Structure of a Honeycomb TCP on Relationship between Bone / Cartilage Formation and Angiogenesis

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