Demonstration of improved tissue integration and angiogenesis with an elastic, estradiol releasing polyurethane material designed for use in pelvic floor repair

Shafaat, Sarah; Mangır, Naşide; Román, Sabiniano; Chapple, Christopher R.; MacNeil, Sheila

doi:10.1002/nau.23510

Cited by 40 publications

(60 citation statements)

References 27 publications

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“…For this purpose, 200 μL of solution was removed from the dialysis medium, added into 96‐well plates, absorbance measured ( n = 5) and the PBS was discarded and replaced with fresh media. The concentration was calculated using a previously plotted calibration curve as described previously …”

Section: Methodsmentioning

confidence: 99%

“…All experiments were performed covered by a Home Office (UK) license. Fertilized chicken eggs ( Gallus gallus domesticus) were purchased (Henry Stewart Co, Ltd, Norfolk, UK) and cultured as described previously using an ex ovo method …”

Section: Methodsmentioning

confidence: 99%

“…Clinically its proangiogenic properties are used in disease states such as cardiac ischaemia and wound healing to improve tissue vascularization. Estradiol has been shown to be a potent stimulator of angiogenesis as released from electrospun materials . Further to this, recent work has suggested that estradiol can be involved in pathophysiologic processes leading to the development of many pelvic floor disorders via its effects on vaginal microcirculation .…”

Section: Introductionmentioning

confidence: 97%

“…Estradiol has been shown to be a potent stimulator of angiogenesis as released from electrospun materials. 11,12 Further to this, recent work has suggested that estradiol can be involved in pathophysiologic processes leading to the development of many pelvic floor disorders via its effects on vaginal microcirculation. 13,14 Accordingly in this study, we wanted to look at the effect of estradiol on microcirculation.…”

Section: Introductionmentioning

confidence: 99%

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An estradiol releasing, proangiogenic hydrogel as a candidate material for use in soft tissue interposition

Mangır

Eke

Hasırcı

et al. 2019

Neurourology and Urodynamics

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Introduction and Objectives Soft tissue interposition (STI) using local and/or regional flaps is often necessary in urogenital reconstruction to stimulate wound healing and prevent recurrence. Harvesting STI flaps can cause donor site morbidity and may not be available in some patients. In this study, we designed estradiol (E2) releasing hydrogel that could be used as an alternative to a STI flap and to investigate its ability to stimulate tissue production and angiogenesis. Materials and Methods A hydrogel was constructed by crosslinking a solution of estradiol, methacrylated gelatin (15%, w/v), and methacrylated hyaluronic acid (1%, w/v). The release of estradiol was measured using a UV‐spectrophotometer (λmax = 220 nm). Angiogenesis was evaluated by an ex ovo chicken embryo chorioallantoic membrane (CAM) assay. Results Estradiol was gradually released from the hydrogel over 21 days. The hydrogels could be easily manipulated with surgical forceps without any deformation. The hydrogels significantly increased collagen production of human dermal fibroblasts (HDFs). Scanning electron microscopic examination demonstrated that HDFs produced significantly more extracellular matrix (ECM) on estradiol releasing hydrogels compared with the controls. Estradiol releasing hydrogels doubled the number of blood vessels growing toward the hydrogel compared with the controls (vasculogenic index, 59.6 [±6.4] and 25.6 [±4.0], respectively; [P < 0.05]). Conclusion We present a proangiogenic, degradable hydrogel that can be used as an off‐the‐shelf available substitute to traditional STI flaps. This is achieved by using estradiol as a potent stimulator of new tissue production and new blood vessel formation.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

An estradiol releasing, proangiogenic hydrogel as a candidate material for use in soft tissue interposition

Mangır

Eke

Hasırcı

et al. 2019

Neurourology and Urodynamics

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“…Similarly to PLA/Ascorbic acid scaffolds, the presence of the releasing agent improves the mechanical properties of the implant, in terms of both strength and elasticity. In addition, the newly developed meshes showed superior biocompatibility (compared to PU scaffolds with no 17‐β‐estradiol), which enhanced the proangiogenic potential of human adipose mesenchymal stem cells and improved tissue integration (Shafaat et al, ). Moreover, the incorporation of different growth factors has been investigated in recent studies.…”

Section: Future Perspectivementioning

confidence: 99%

The use of polymeric meshes for pelvic organ prolapse: Current concepts, challenges, and future perspectives

et al. 2019

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Pelvic organ prolapse (POP) is one of the most common chronic disorders in women, impacting the quality of life of millions of them worldwide. More than 100 surgical procedures have been developed over the decades to treat POP. However, the failure of conservative strategies and the number of patients with recurrence risk have increased the need for further adjuvant treatments. Since their introduction, surgical synthetic meshes have dramatically transformed POP repair showing superior anatomic outcomes in comparison to traditional approaches. Although significant progress has been attained, among the meshes in clinical use, there is no single mesh appropriate for every surgery. Furthermore, due to the risk of complications including acute and chronic infection, mesh shrinkage, and erosion of the tissue, the benefits of the use of meshes have recently been questioned. The aim of this work is to review the evolution of POP surgery, analyzing the current challenges, and detailing the key factors pertinent to the design of new mesh systems. Starting with a description of the pelvic floor anatomy, the article then presents the traditional treatments used in pelvic organ disorders. Next, the development of synthetic meshes is described with an insight into how their function is dependent on both mesh design variables (i.e., material, structure, and functional treatment) and surgical applications. These are then linked to common mesh‐related complications, and an indication of current research aiming to address these issues.

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Mechanoresponsive Drug Release from a Flexible, Tissue‐Adherent, Hybrid Hydrogel Actuator

et al. 2023

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Soft robotic technologies for therapeutic biomedical applications require conformal and atraumatic tissue coupling that is amenable to dynamic loading for effective drug delivery or tissue stimulation. This intimate and sustained contact offers vast therapeutic opportunities for localized drug release. Herein, a new class of hybrid hydrogel actuator (HHA) that facilitates enhanced drug delivery is introduced. The multi‐material soft actuator can elicit a tunable mechanoresponsive release of charged drug from its alginate/acrylamide hydrogel layer with temporal control. Dosing control parameters include actuation magnitude, frequency, and duration. The actuator can safely adhere to tissue via a flexible, drug‐permeable adhesive bond that can withstand dynamic device actuation. Conformal adhesion of the hybrid hydrogel actuator to tissue leads to improved mechanoresponsive spatial delivery of the drug. Future integration of this hybrid hydrogel actuator with other soft robotic assistive technologies can enable a synergistic, multi‐pronged treatment approach for the treatment of disease.

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Demonstration of improved tissue integration and angiogenesis with an elastic, estradiol releasing polyurethane material designed for use in pelvic floor repair

Abstract: This study shows the angiogenic potential of estradiol-releasing PU scaffolds with appropriate strength and elasticity desirable to support the pelvic floor.

Cited by 40 publications

References 27 publications

An estradiol releasing, proangiogenic hydrogel as a candidate material for use in soft tissue interposition

An estradiol releasing, proangiogenic hydrogel as a candidate material for use in soft tissue interposition

The use of polymeric meshes for pelvic organ prolapse: Current concepts, challenges, and future perspectives

Mechanoresponsive Drug Release from a Flexible, Tissue‐Adherent, Hybrid Hydrogel Actuator

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