Characterization of subgroup J avian Leukosis virus isolated from Chinese indigenous chickens

Meng, Fanjuan; Li, Qiuchen; Zhang, Yawen; Zhang, Zhihui; Tian, Sibao; Cui, Zhizhong; Chang, Shuang; Zhao, Peng

doi:10.1186/s12985-018-0947-1

Cited by 30 publications

(26 citation statements)

References 22 publications

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“…The gp85, gp37, E element and 3′UTR genes of isolated ALV‐J strains were amplified by PCR with the specific primers respectively (Meng et al., ). The PCR products were sequenced by the Huada Genetic Sequence Company (Beijing, China).…”

Section: Methodsmentioning

confidence: 99%

“…To reproduce the pathogenicity of the newly isolated ALV-J strains, 70 1-day-old specific pathogen free (SPF) chicks, purchased The gp85, gp37, E element and 3′UTR genes of isolated ALV-J strains were amplified by PCR with the specific primers respectively (Meng et al, 2018). The PCR products were sequenced by the Huada Genetic Sequence Company (Beijing, China).…”

Section: Methodsmentioning

confidence: 99%

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Outbreak of myelocytomatosis caused by mutational avian leukosis virus subgroup J in China, 2018

Zhou

Xue

Zhang

et al. 2018

Transbound Emerg Dis

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Avian leukosis virus subgroup J (ALV‐J) was isolated in meat‐type breeder chickens for the first time in 1988 in the United Kingdom. Due to the application of an eradication program, there were fewer reports related to myelocytomatosis or ALV‐J in China after 2013. However, there was another breakout almost simultaneously in six provinces of China in February 2018. On‐site, 15‐ to 20‐week‐old broiler breeder chickens showed depression, paralysis and weight loss. Mortality for certain flocks reached 15%. Sick chickens showed numerous yellow–white neoplasms growing in the sternum, rib and lumbar vertebra and had hepatic and renal metastasis. Histopathological observation showed all neoplasms were myelocytomas, and there were massive myelocyte‐like tumour cells in the liver, kidney and bone marrow. To explore the aetiology of this re‐outbreak of myelocytomatosis in China, we collected tumour‐bearing chickens and isolated six strains of ALV‐J (GM0209‐1 to ‐6). Phylogenetic analysis of gp85 and gp37 showed GM0209 strains were clearly distinct from the prototype strain of ADOL‐7501, HPRS‐103 and NX0101, and there was a mutation, R176G, in the conserved region between hr1 and hr2 regions of gp85, which was not found in other 44 ALV‐J strains. The 3′UTR nucleotide sequences of GM0209 isolates showed there was a signature deletion of 11 nt that was also present in 3′UTR sequences of SCDY1 and NHH, two isolates that have a reported association with haemangioma, indicating this deletion could not determine the tumour type induced by ALV‐J. Although the eradication program of ALV‐J has been successfully applied in China, the outbreak of ALV‐J still occurred, and the virus strain spread quickly. Thus, the biocharacteristics and pathogenesis of mutational ALV‐J should be further studied.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Outbreak of myelocytomatosis caused by mutational avian leukosis virus subgroup J in China, 2018

Zhou

Xue

Zhang

et al. 2018

Transbound Emerg Dis

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“…ALVs are classified into seven subgroups, referred to as A-E, J and K (16). Among these seven subgroups, ALV-J is the most prevalent and causes more damage to chickens compared with the other subgroups (17). Furthermore, ALV-J infection causes immunosuppression and various forms of myeloid leukosis and, to a lesser extent, solid tumors, such as hemangiomas and histiocytic sarcomas (12).…”

Section: Introductionmentioning

confidence: 99%

gga‑microRNA‑375 negatively regulates the cell cycle and proliferation by targeting Yes‑associated protein 1 in DF‑1 cells

Zhang

et al. 2020

Exp Ther Med

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MicroRNAs (miRNAs/miRs) serve a key role in regulating the cell cycle and inducing tumorigenesis. Subgroup J of the avian leukosis virus (ALV-J) belongs to the family Retroviridae, subfamily Orthoretrovirinae and genus Alpharetrovirus that causes tumors in susceptible chickens. gga-miR-375 is downregulated and Yes-associated protein 1 (YAP1) is upregulated in ALV-J-induced tumors in the livers of chickens, and it has been further identified that YAP1 is the direct target gene of gga-miR-375. In the present study, it was found that ALV-J infection promoted the cell cycle and proliferation in DF-1 cells. As the cell cycle and cell proliferation are closely associated with tumorigenesis, further experiments were performed to determine whether gga-miR-375 and YAP1 were involved in these cellular processes. It was demonstrated that gga-miR-375 significantly inhibited the cell cycle by inhibiting G 1 to S/G 2 stage transition and decreasing cell proliferation, while YAP1 significantly promoted the cell cycle and proliferation. Furthermore, these cellular processes in DF-1 cells were affected by gga-miR-375 through the targeting of YAP1. Collectively, the present results suggested that gga-miR-375, downregulated by ALV-J infection, negatively regulated the cell cycle and proliferation via the targeting of YAP1.

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“…ALV-J infection of broilers was first detected in China in 1999. In the past decade, the host range of ALV-J has gradually expanded to commercial layers, the Chinese local breeds [2][3][4], suggesting that ALV-J infection is a major problem in China.…”

Section: Introductionmentioning

confidence: 99%

Avian leukosis virus subgroup J infection influencing composition of gut microbiota within chicken

Chen

2020

Preprint

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Background: Avian leukosis virus (ALV) is a major cause of disease in poultry. Probiotics play a critical role in animal health maintenance. Studies have indicated that viral infection can alter the composition of the chicken gut microbiota. We hypothesized that ALV-J infection alters the probiotics composition in chicken fecal bacterial microbiome. We performed high-throughput 16S rRNA gene sequencing and evaluated the gut microbiota proﬁles using feces from ALV-J-infected and healthy chickens. Results: The relative abundance at the phylum and species levels was calculated. The phylum Proteobacteria was more abundant in ALV-J-infected chickens than in healthy chickens. Additionally, the abundance of the opportunistic pathogen, Propionibacterium acnes, was significantly increased in ALV-J-infected chickens. Interestingly, ALV-J infection tended to be significantly decreased by the probiotics Lactobacillus helveticus and Lactobacillus reuteri. Conclusions: The study indicates that ALV-J infection significantly altered the gut microbiota distribution in chickens. Additionally, ALV-J infection significantly influenced the abundance of L. helveticus and L. reuteri in the chicken gut.

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Characterization of subgroup J avian Leukosis virus isolated from Chinese indigenous chickens

Cited by 30 publications

References 22 publications

Outbreak of myelocytomatosis caused by mutational avian leukosis virus subgroup J in China, 2018

Outbreak of myelocytomatosis caused by mutational avian leukosis virus subgroup J in China, 2018

gga‑microRNA‑375 negatively regulates the cell cycle and proliferation by targeting Yes‑associated protein 1 in DF‑1 cells

Avian leukosis virus subgroup J infection influencing composition of gut microbiota within chicken

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