[11C]Harmine Binding to Brain Monoamine Oxidase A: Test-Retest Properties and Noninvasive Quantification

Abstract

Prospective studies using [C]harmine are possible given its test-retest repeatability when binding is quantified using arterial blood. Results with SIME of input function show potential for simplifying data acquisition by replacing arterial catheterization with one arterial blood sample at 20 min post-injection. Estimation of [C]harmine binding potentials remains a challenge that warrants further investigation.

Elevated Monoamine Oxidase-A in Anterior Cingulate of Post-Mortem Human Parkinson’s Disease: A Potential Surrogate Biomarker for Lewy Bodies?

“…Reversible MAO-A PET radiotracer [ 11 C]Harmine has been used to study depression [ 19 ] and [ 18 F]FEH [ 20 ] has only been used in animal imaging. Quantitative human PET studies using [ 11 C]harmine have been carried out [ 21 ]. To the best of our knowledge, PET imaging of MAO-A in PD has not been reported.…”

Radiosynthesis, Preclinical, and Clinical Positron Emission Tomography Studies of Carbon-11 Labeled Endogenous and Natural Exogenous Compounds

“…172 Due to its promising properties as a CNS MAO-A imaging agent, it was further evaluated for binding quantification and measuring the effects of competing substrates in healthy volunteers. 116,117 [Methoxy-11 C]harmine has been evaluated as a radiotracer to detect MAO-A levels in neuropsychiatric disorders. In people affected by antisocial personality disorder, [methoxy-11 C]harmine PET analyses revealed reduced functional MAO-A levels in all brain regions investigated (e.g., orbitofrontal and prefrontal cortex).…”

“…Treatment with MAO-A inhibitors such as moclobemide induces enzyme blocking at the peripheral site resulting in increased radioactivity in the brain . Due to its promising properties as a CNS MAO-A imaging agent, it was further evaluated for binding quantification and measuring the effects of competing substrates in healthy volunteers. , …”

Source‐to‐Target Automatic Rotating Estimation (STARE) – A publicly‐available, blood‐free quantification approach for PET tracers with irreversible kinetics: Theoretical framework and validation for [18F]FDG

“…SIME of the input function achieves less-invasive quantification by fitting the proper tissue compartment model to multiple brain regions’ TACs simultaneously. This allows the free parameters of the model, that is the parameters requiring estimation, to be estimated simultaneously, under the usual assumption that the AIF is the input function common to all regions ( Guo et al, 2007 ; Wong et al, 2002 ; Wong et al, 2001 ; Ogden et al, 2010 ; Bohorquez, 2020 ; Riabkov and Di Bella, 2002 ; Feng et al, 1997 ; Sari, 2018 ; Zanderigo, 2018 ; Maroy et al, 2020 ). In SIME of the input function, the model free parameters are both those describing the tracer kinetics in the tissue (e.g., for an irreversible tracer, the micro-parameters K 1 , k 2 , and k 3 for each brain region) and those describing the AIF (e.g., the parameters of the model often used for the AIF, which is the sum of three decreasing exponentials).…”