Changes in oxidative stress severity and antioxidant potential during muscle atrophy and reloading in mice

Katsuta, Wakana; Aihara, Masahiro; Hirose, Noboru; Saito, Fumiaki; Hagiwara, Hiroki

doi:10.1589/jpts.30.42

Cited by 3 publications

(2 citation statements)

References 16 publications

(17 reference statements)

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“…The d-ROMs test is simple method of measuring the free radical levels in vivo and a high correlation with other oxidative stress markers [ 15 ]. At the beginning of the experiment, plasma d-ROMs levels of the BL/O and KK/O groups were 88.6 ± 2.2 and 134.8 ± 8.4 U. CARR, respectively.…”

Section: Resultsmentioning

confidence: 99%

Phenotypic and genotypic changes in obesity and type 2 diabetes of male KK mice with aging

et al. 2022

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Research into the prevention and treatment of age-related metabolic diseases are important in the present-day situation of the aging population. We propose that an elderly diabetic mouse model may be useful to such research as it exhibits deterioration of glucose and lipid metabolism. Although the KK mouse strain is commonly used as a model of moderate obesity and type 2 diabetes, the utility of this strain as an elderly obese and diabetic model mouse for research into aging remains unclear. The present study aimed to investigate age-related changes of glucose and lipid metabolism in male KK mice fed a standard chow diet.We demonstrate that 40 weeks KK mice exhibit age-related dysfunctions, such as development of insulin resistance associated with pancreatic islet hypertrophy and decreased lipolysis in white adipose tissue (WAT) compared with 15 weeks KK mice. However, aging does not appear to cause mitochondrial dysfunction of brown adipose tissue. Unexpectedly, hyperglycemia, potential glucose uptake in insulin-sensitive organs, hepatic lipid accumulation, hypertrophy of adipocytes, and inflammation in epididymal WAT did not worsen but rather compensated in 40 weeks KK mice. Our data indicate that the use of male KK mice as an elderly obese and diabetic mouse model has some limitations and in order to represent a useful elderly obese and diabetic animal model, it may be necessary to induce deterioration of glucose and lipid metabolism in KK mice through breeding with high-sucrose or high-fat diets.

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Section: Resultsmentioning

confidence: 99%

Phenotypic and genotypic changes in obesity and type 2 diabetes of male KK mice with aging

et al. 2022

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“…There are two main sources of ROS in muscle fibers: 1) mitochondria that form ROS during incomplete coupling of oxidation and phosphorylation [ 70 ] and NADPH-oxidase-2 (NOX-2), which localizes in the sarcolemma and internal membranes (for refs see [ 71 ]). During long-time exposure to unloading, an increased formation of ROS in skeletal muscle is observed [ 72 ]. There is less data on the accumulation of ROS in the first hours/days of unloading and these data are contradictory.…”

Section: E3-ubiquitin Ligase Expression Control Irs-1 Degradationmentioning

confidence: 99%

How Postural Muscle Senses Disuse? Early Signs and Signals

Шенкман

2020

IJMS

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A mammalian soleus muscle along with other “axial” muscles ensures the stability of the body under the Earth’s gravity. In rat experiments with hindlimb suspension, zero-gravity parabolic flights as well as in human dry immersion studies, a dramatic decrease in the electromyographic (EMG) activity of the soleus muscle has been repeatedly shown. Most of the motor units of the soleus muscle convert from a state of activity to a state of rest which is longer than under natural conditions. And the state of rest gradually converts to the state of disuse. This review addresses a number of metabolic events that characterize the earliest stage of the cessation of the soleus muscle contractile activity. One to three days of mechanical unloading are accompanied by energy-dependent dephosphorylation of AMPK, accumulation of the reactive oxygen species, as well as accumulation of resting myoplasmic calcium. In this transition period, a rapid rearrangement of the various signaling pathways occurs, which, primarily, results in a decrease in the rate of protein synthesis (primarily via inhibition of ribosomal biogenesis and activation of endogenous inhibitors of mRNA translation, such as GSK3β) and an increase in proteolysis (via upregulation of muscle-specific E3-ubiquitin ligases).

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Effects of inactivity and exercise intervention on brain-derived neurotrophic factor in mice: Comparison of kinetics in serum, skeletal muscle, and brain

MIKI,

AIHARA,

KAWAGUCHI

et al. 2024

Biomed. Res.

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Exercise training increases brain-derived neurotrophic factor (BDNF) expression and improves cognitive function. However, the dynamics of BDNF during inactivity and the effects of exercise intervention on BDNF levels have rarely been examined. Therefore, we aimed to examine changes in serum, skeletal muscle, and brain BDNF levels under these conditions. Mice were divided into control (Co), cast immobilization (CI), reloading (RL), and exercise (Ex) groups. Muscle atrophy was induced by cast immobilization for 2 weeks in the CI, RL, and Ex groups. After cast removal, the RL and Ex groups underwent regrounding and treadmill exercise, respectively, for 2 weeks. Serum, skeletal muscle, and brain BDNF levels showed a similar decreasing trend in the CI group, recovery in the RL group, and a further increase in the Ex group compared with those in the Co group. This indicates that BDNF levels change in parallel with the degree of activity. However, the magnitude of variation differed among the tissues in the order of serum > skeletal muscle > brain tissue. These results suggest that different mechanisms in different tissues regulate BDNF expression. BDNF could potentially act as an objective measure of the impact of both inactivity and exercise-based interventions.

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Changes in oxidative stress severity and antioxidant potential during muscle atrophy and reloading in mice

Cited by 3 publications

References 16 publications

Phenotypic and genotypic changes in obesity and type 2 diabetes of male KK mice with aging

Phenotypic and genotypic changes in obesity and type 2 diabetes of male KK mice with aging

How Postural Muscle Senses Disuse? Early Signs and Signals

Effects of inactivity and exercise intervention on brain-derived neurotrophic factor in mice: Comparison of kinetics in serum, skeletal muscle, and brain

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