Rationalizing and advancing the 3-MPBA SERS sandwich assay for rapid detection of bacteria in environmental and food matrices

Pearson, Brooke; Mills, Alexander; Tucker, Madeline; Gao, Siyue; McLandsborough, Lynne; He, Lili

doi:10.1016/j.fm.2017.11.007

Cited by 30 publications

(22 citation statements)

References 31 publications

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“…These vibrational assignments reflect phenol ring stretching among the chemical label 16 . The phenyl ring group among 3‐MPBA produces very robust SERS peaks that are consistent with our previous investigations 13,14 . When bacteria are involved with AuNPs and 3‐MPBA, Raman shifting produced one prominent peak at 1024 cm −1 that differed from the negative control.…”

Section: Resultssupporting

confidence: 88%

“…3-Mercaptophenylboronic acid produces light contrasting effects when bound to bacteria cells that aid in their optical analyses. 13,14 F I G U R E 2 Electron micrographs of the bacteria samples as they were analyzed using SERS. A, Nanoparticles exhibited less contact with label-free cells than labeled cells, resulting in an abundance of free nanoparticles across the substrate.…”

Section: Sers Images Of Label-free Versus Labeled Bacteria Cellsmentioning

confidence: 99%

“…These conditions fortunately align when using boronic acids to measure bacteria cell populations using SERS. The efficacy of boronic acid‐based bacterial SERS labels were proven through a variety of studies and show remarkable promise for interdisciplinary applications throughout the field 12–14 . The boronic esters were utilized as a capturing agent that can anchor bacteria to a surface for SERS mapping and their Raman spectra were analyzed for statistical variation among bacteria cells.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of label‐free and label‐based approaches for surface‐enhanced Raman microscopic imaging of bacteria cells

Hickey

Gao

2020

Analytical Science Advances

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Surface-enhanced Raman spectroscopic (SERS) approaches are emerging for bacteria analysis whereby bacteria cells can be measured based on their biochemical composition (label-free) or with the aid of a chemical label to enhance the SERS signal. Combining a microscope, SERS microscopy is capable of imaging bacteria populations en masse based on specific spectrophotometric peaks. Here, we compared the label-free and label-based approaches to study Escherichia coli O157:H7 that was utilized as a model bacterium for SERS imaging analyses. Gold (Au) nanoparticles were utilized to enhance Raman scattering during this study and 3-mercaptophenylboronic acid was utilized as a model chemical label for comparison against label-free conditions. The result shows that SERS images of bacteria cells yielded measurable differences in precision, depending on the application of chemical labels. Chemical labels enabled SERS imaging of whole bacteria populations with single-cell precision. Bacteria coated with labels were also easier to bring into focus using high-magnification optical microscopy, without the need for immersion oil. Label-free analyses of single-cells were lower in geographic precision but provided opportunities to study the natural biochemistry of bacteria cells with strong accuracy. SERS analyses of label-free bacteria cell components were conclusively improved in vitro on a time-dependent basis. This concept can serve as an important benchmark when biochemically profiling or characterizing bacteria cells based on SERS. Electron micrographs proved that chemical labels can be utilized to increase nanoparticle contact with bacteria cells and reduce free nanoparticles that contribute to background noise in SERS spectra. We also demonstrate the use of both 3-mercaptophenylboronic acid and propidium iodide to discriminate live and dead bacteria through the simultaneous collection of data from these two chemical labels. Label-free approaches to SERS bacteria analyses are better suited for biochemical characterization and label-based approaches are better suited when accounting for individual cells among a population.

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Section: Resultssupporting

confidence: 88%

Section: Sers Images Of Label-free Versus Labeled Bacteria Cellsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparison of label‐free and label‐based approaches for surface‐enhanced Raman microscopic imaging of bacteria cells

Hickey

Gao

2020

Analytical Science Advances

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“…However, the APC method has more advantages in terms of cell viability. [149] Zheng et al synthesized a multifunctional gold nanobones (GNBs) based on gold nanorods (GNRs) as SERS enhanced substrates, which were modified by aptamer-1 (Apt-1) and used to detect E. coli O157: H7 by signal molecule rhodamine B (RhB). Among them, Apt-1 and RhB are uniformly embedded in the GNBs structure, and the detection results show that the SERS signal has high selectivity, high stability, and high sensitivity.…”

Section: Application Of Bacterial Detection In Clinical and Food Safetymentioning

confidence: 99%

“…However, the APC method has more advantages in terms of cell viability. [ 149 ] Zheng et al. synthesized a multifunctional gold nanobones (GNBs) based on gold nanorods (GNRs) as SERS enhanced substrates, which were modified by aptamer‐1 (Apt‐1) and used to detect E. coli O157: H7 by signal molecule rhodamine B (RhB).…”

Section: Application Of Bacterial Detection In Clinical and Food Safetymentioning

confidence: 99%

Bacteria Detection: From Powerful SERS to Its Advanced Compatible Techniques

et al. 2020

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The rapid, highly sensitive, and accurate detection of bacteria is the focus of various fields, especially food safety and public health. Surface-enhanced Raman spectroscopy (SERS), with the advantages of being fast, sensitive, and nondestructive, can be used to directly obtain molecular fingerprint information, as well as for the on-line qualitative analysis of multicomponent samples. It has therefore become an effective technique for bacterial detection. Within this progress report, advances in the detection of bacteria using SERS and other compatible techniques are discussed in order to summarize its development in recent years. First, the enhancement principle and mechanism of SERS technology are briefly overviewed. The second part is devoted to a label-free strategy for the detection of bacterial cells and bacterial metabolites. In this section, important considerations that must be made to improve bacterial SERS signals are discussed. Then, the label-based SERS strategy involves the design strategy of SERS tags, the immunomagnetic separation of SERS tags, and the capture of bacteria from solution and dye-labeled SERS primers. In the third part, several novel SERS compatible technologies and applications in clinical and food safety are introduced. In the final part, the results achieved are summarized and future perspectives are proposed.

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Boosting bacteria differentiation efficiency with multidimensional surface‐enhanced Raman scattering: the example of Bacillus cereus

Zhu

Wang

et al. 2022

Luminescence

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Surface-enhanced Raman scattering (SERS) is a powerful tool for constructing biomolecular fingerprints, which play a vital role in differentiation of bacteria. Due to the rather subtle differences in the SERS spectra among different bacteria, artificial intelligence is usually adopted and enormous amounts of spectral data are required to improve the differentiation efficiency. However, in many cases, large volume data acquisition on bacteria is not only technical difficult but labour intensive. It is known

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Rationalizing and advancing the 3-MPBA SERS sandwich assay for rapid detection of bacteria in environmental and food matrices

Cited by 30 publications

References 31 publications

Comparison of label‐free and label‐based approaches for surface‐enhanced Raman microscopic imaging of bacteria cells

Comparison of label‐free and label‐based approaches for surface‐enhanced Raman microscopic imaging of bacteria cells

Bacteria Detection: From Powerful SERS to Its Advanced Compatible Techniques

Boosting bacteria differentiation efficiency with multidimensional surface‐enhanced Raman scattering: the example of Bacillus cereus

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