One after the other: A novel Bluetongue virus strain related to Toggenburg virus detected in the Piedmont region (North-western Italy), extends the panel of novel atypical BTV strains

Marcacci, Maurilia; Sant, Serena; Mangone, Iolanda; Goria, Maria; Dondo, Alessandro; Zoppi, Simona; Gennip, René G. P. van; Radaelli, Maria Cristina; Cammà, Cesare; Rijn, Piet A. van; Savini, Giovanni; Lorusso, Alessio

doi:10.1111/tbed.12822

Cited by 45 publications

(37 citation statements)

References 27 publications

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“…NGS was used to obtain partial sequences directly from blood samples, as well as complete coding sequences from isolated virus. This showed high levels of identity between the BTV TUN2016 and the BTV‐3 SAR2018 strains confirming that NGS is becoming a central and crucial diagnostic technique enabling the identification and characterization of a given BTV strain (and thus its potential origin) in a rapid time period as described in previous reports (Marcacci et al., ; Savini et al., ). This aspect, combined with the availability of portable third generation sequencers (Beato et al., ; Peserico et al., ) would make possible the molecular diagnosis of BTV also in field conditions.…”

supporting

confidence: 80%

Western Bluetongue virus serotype 3 in Sardinia, diagnosis and characterization

Cappai

Rolesu

Loi

et al. 2019

Transbound Emerg Dis

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Over the last 20 years, Italy has experienced multiple incursions of different serotypes of Bluetongue virus (BTV), a Culicoides‐borne arbovirus, the causative agent of bluetongue (BT), a major disease of ruminants. The majority of these incursions originated from Northern Africa, likely because of wind‐blown dissemination of infected midges. Here, we report the first identification of BTV‐3 in Sardinia, Italy. BTV‐3 circulation was evidenced in sentinel animals located in the province of Sud Sardegna on September 19, 2018. Prototype strain BTV‐3 SAR2018 was isolated on cell culture. BTV‐3 SAR2018 sequence and partial sequences obtained by next‐generation sequencing from nucleic acids purified from the isolate and blood samples, respectively, were demonstrated to be almost identical (99–100% of nucleotide identity) to BTV‐3 TUN2016 identified in Tunisia in 2016 and 2017, a scenario already observed in past incursions of other BTV serotypes originating from Northern Africa.

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supporting

confidence: 80%

Western Bluetongue virus serotype 3 in Sardinia, diagnosis and characterization

Cappai

Rolesu

Loi

et al. 2019

Transbound Emerg Dis

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“…BTV is a double-stranded (ds) RNA virus (3), its genome consists of ten segments (Seg-1 to Seg-10) of linear dsRNA coding 7 structural (VP1-VP7) and 5 non-structural (NS1, NS2, NS3/NS3a, NS4, and NS5) proteins (4) At present twenty-eighth distinct BTV serotypes have been officially recognized based on Seg-2 gene sequence (5,6). Other putative novel BTV serotypes have also been described (7)(8)(9)(10)(11).…”

Section: Introductionmentioning

confidence: 99%

Bluetongue Serotype 3 in Israel 2013–2018: Clinical Manifestations of the Disease and Molecular Characterization of Israeli Strains

Golender¹,

Bumbarov²,

Eldar³

et al. 2020

Front. Vet. Sci.

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“…Currently, there are 24 classic serotypes of the virus, all capable of causing BT, plus a series of new serotypes, defined as atypical because infected animals are asymptomatic (21)(22)(23)(24)(25)(26)(27).…”

Section: Introductionmentioning

confidence: 99%

Development of a Digital RT-PCR Method for Absolute Quantification of Bluetongue Virus in Field Samples

et al. 2020

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Bluetongue (BT) is a major Office International des Epizooties (OIE)-listed disease of wild and domestic ruminants caused by several serotypes of Bluetongue virus (BTV), a virus with a segmented dsRNA genome belonging to the family Reoviridae, genus Orbivirus. BTV is transmitted through the bites of Culicoides midges. The aim of this study was to develop a new method for quantification of BTV Seg-10 by droplet digital RT-PCR (RTdd-PCR), using nucleic acids purified from complex matrices such as blood, tissues, and midges, that notoriously contain strong PCR inhibitors. First, RTdd-PCR was optimized by using RNAs purified from serially 10-fold dilutions of a BTV-1 isolate (10 5.43 TCID 50 /ml up to 10 −0.57 TCID 50 /ml) and from the same dilutions spiked into fresh ovine EDTA-blood and spleen homogenate. The method showed a good degree of linearity (R 2 ≥ 0.995). The limit of detection (LoD) and the limit of quantification (LoQ) established were 10 −0.67 TCID 50 /ml (0.72 copies/µl) and 10 0.03 TCID 50 /ml (3.05 copies/µl) of BTV-1, respectively. Second, the newly developed test was compared, using the same set of biological samples, to the quantitative RT-PCR (RT-qPCR) detecting Seg-10 assay widely used for the molecular diagnosis of BTV from field samples. Results showed a difference mean of 0.30 log between the two assays with these samples (p < 0.05). Anyway, the analysis of correlation demonstrated that both assays provided similar measurements with a very close agreement between the systems.

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One after the other: A novel Bluetongue virus strain related to Toggenburg virus detected in the Piedmont region (North-western Italy), extends the panel of novel atypical BTV strains

Cited by 45 publications

References 27 publications

Western Bluetongue virus serotype 3 in Sardinia, diagnosis and characterization

Western Bluetongue virus serotype 3 in Sardinia, diagnosis and characterization

Bluetongue Serotype 3 in Israel 2013–2018: Clinical Manifestations of the Disease and Molecular Characterization of Israeli Strains

Development of a Digital RT-PCR Method for Absolute Quantification of Bluetongue Virus in Field Samples

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