Long Noncoding RNA GAS5 Promotes Proliferation, Migration, and Invasion by Regulation of miR-301a in Esophageal Cancer

Li, Wei; Zhao, Weidong; Lu, Zhanwu; Zhang, Wen; Yang, Xuan

doi:10.3727/096504018x15166193231711

Cited by 42 publications

(28 citation statements)

References 23 publications

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“…Furthermore, the GAS5 /miR-21/ PTEN axis influences cisplatin resistance and chemosensitivity, in cervical cancer and non-small cell lung cancer (NSCLC), respectively [ 66 , 67 ]. The only study demonstrating oncogenic properties of GAS5 showed that it upregulated CXCR4 (C-X-C motif chemokine receptor 4) by competing for miR-301a, in turn activating Wnt/β-catenin and NF-κB (nuclear factor kappa B) signaling to promote proliferation, migration, and invasion in esophageal cancer [ 68 ], suggesting that GAS5 could exert opposing functions in a tissue-specific manner.…”

Section: The Links Between Long Noncoding Rnas and Micrornasmentioning

confidence: 99%

Noncoding RNA:RNA Regulatory Networks in Cancer

Chan

Tay

2018

IJMS

917

735

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Noncoding RNAs (ncRNAs) constitute the majority of the human transcribed genome. This largest class of RNA transcripts plays diverse roles in a multitude of cellular processes, and has been implicated in many pathological conditions, especially cancer. The different subclasses of ncRNAs include microRNAs, a class of short ncRNAs; and a variety of long ncRNAs (lncRNAs), such as lincRNAs, antisense RNAs, pseudogenes, and circular RNAs. Many studies have demonstrated the involvement of these ncRNAs in competitive regulatory interactions, known as competing endogenous RNA (ceRNA) networks, whereby lncRNAs can act as microRNA decoys to modulate gene expression. These interactions are often interconnected, thus aberrant expression of any network component could derail the complex regulatory circuitry, culminating in cancer development and progression. Recent integrative analyses have provided evidence that new computational platforms and experimental approaches can be harnessed together to distinguish key ceRNA interactions in specific cancers, which could facilitate the identification of robust biomarkers and therapeutic targets, and hence, more effective cancer therapies and better patient outcome and survival.

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Section: The Links Between Long Noncoding Rnas and Micrornasmentioning

confidence: 99%

Noncoding RNA:RNA Regulatory Networks in Cancer

Chan

Tay

2018

IJMS

917

735

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“…It could sponge MIR301A to affect Wnt and NF-kB signaling pathways to promote cancer cell proliferation, migration and invasion but reduced cell apoptosis. 103 However, Ke et al insisted that GAS5 was an anticancer gene in esophageal tumor. Overexpression of GAS5 significantly impeded tumorigenesis via EMT.…”

Section: Snhgs In Esophageal Cancermentioning

confidence: 99%

Long non‐coding small nucleolar RNA host genes in digestive cancers

et al. 2019

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Although long noncoding RNAs (lncRNAs) do not have protein coding capacities, they are involved in the pathogenesis of many types of cancers, including hepatocellular carcinoma, cervical cancer, and gastric cancer. Notably, the roles of lncRNAs are vital in nearly every aspect of tumor biology. Long non‐coding small nucleolar RNA host genes (lnc‐SNHGs) are abnormally expressed in multiple cancers, including urologic neoplasms, respiratory tumors, and digestive cancers, and play vital roles in these cancers. These host genes could participate in tumorigenesis by regulating proliferation, migration, invasion and apoptosis of tumor cells. This review focuses on the overview of the roles that lnc‐SNHGs play in the formation and progression of digestive cancers.

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“…GAS5 could interact with miRNAs to participate in the process of tumorigenesis [27]. Hence, we found that GAS5 could interact with miR-23a-3p through luciferase reporter gene and RNA immunoprecipitation.…”

Section: Discussionmentioning

confidence: 82%

LncRNA GAS5 suppresses the proliferation and invasion of osteosarcoma cells via the miR-23a-3p/PTEN/PI3K/AKT pathway

Liu¹,

Chen²,

Ma³

et al. 2020

Preprint

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Background: Accumulating evidence has shown that lncRNA growth arrest special 5 (GAS5) is a well‑known tumor suppressor in the pathogenesis of a variety of human cancers. However, the detailed role of GAS5 in osteosarcoma is largely unclear. Here, we explore the role of GAS5 in progression of osteosarcoma. Methods: The expression level of GAS5 was detected in human osteosarcoma tissues and matched adjacent tissues, as well as osteosarcoma cell lines and non-malignant osteoblast cells. Then, in vitro gain- and loss-of-function experiments, with the pcDNA-GAS5 expression vector and GAS5-siRNA, were performed in U2OS and HOS cells to determine the effect of GAS5 on osteosarcoma cell proliferation and invasion. Subsequently, we searched potential miRNA targets with bioinformatics analysis and confirmed their interaction by using luciferase reporter gene and RNA pull-down assays. The function and mechanism of miR-23a-3p in proliferation and invasion was also investigated in U2OS and HOS cells. Furthermore, rescue experiments were performed to verify the involvement of miR-23a-3p and its target gene in GAS5-mediated cell behaviors. Finally, a xenograft nude mouse model was established by subcutaneous injection with U2OS cells overexpressing GAS5 or not, and the effect of GAS5 on tumor growth in vivo was evaluated. Results: GAS5 was downregulated in human osteosarcoma tissues and cell lines. Overexpression of GAS5 could significantly suppress, and downregulation of GAS5 promoted, proliferation and invasion of osteosarcoma cells. GAS5 could directly bind with and downregulated miR-23a-3p that post-transcriptionally downregulated the tumor suppressor PTEN and positively regulated proliferation and invasion of osteosarcoma cells. Rescue experiments confirmed the involvement of miR-23a-3p and PTEN in GAS5-mediated cell behaviors by modifying the phosphatidylinositol-3-kinases/protein-serine-threonine kinase (PI3K/AKT) pathway. GAS5 could inhibit tumor growth in vivo . Conclusion: GAS5 functions as a competing endogenous RNA , sponging miR-23a-3p, to promote PTEN expression and suppress cell growth and invasion in osteosarcoma by regulating the PI3K/AKT pathway.

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Long Noncoding RNA GAS5 Promotes Proliferation, Migration, and Invasion by Regulation of miR-301a in Esophageal Cancer

Cited by 42 publications

References 23 publications

Noncoding RNA:RNA Regulatory Networks in Cancer

Noncoding RNA:RNA Regulatory Networks in Cancer

Long non‐coding small nucleolar RNA host genes in digestive cancers

LncRNA GAS5 suppresses the proliferation and invasion of osteosarcoma cells via the miR-23a-3p/PTEN/PI3K/AKT pathway

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