Production of lutein, and polyunsaturated fatty acids by the acidophilic eukaryotic microalga Coccomyxa onubensis under abiotic stress by salt or ultraviolet light

Bermejo, Elisabeth; Ruiz-Domínguez, Mari Carmen; Cuaresma, María; Vaquero, Isabel; Ramos-Merchante, Adrián; Vega, José M.; Vílchez, Carlos; Garbayo, Inés

doi:10.1016/j.jbiosc.2017.12.025

Cited by 57 publications

(22 citation statements)

References 49 publications

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“…; Bermejo et al. ; Blanco et al. ), our results reveal a rare case where a microalga can produce the β‐carotene family from this pathway.…”

Section: Discussionsupporting

confidence: 58%

“…strain TISTR 9500 was shifted to the production of compounds in the b-carotene family (Borowitzka 2013). In comparison to previous reports utilizing related microalgae (Ambati et al 2010;Bermejo et al 2018;Blanco et al 2007), our results reveal a rare case where a microalga can produce the b-carotene family from this pathway. Thus, Chlorococcum sp.…”

Section: Discussioncontrasting

confidence: 54%

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Effects of Potassium Chloride‐Induced Stress on the Carotenoids Canthaxanthin, Astaxanthin, and Lipid Accumulations in the Green Chlorococcal Microalga Strain TISTR 9500

Janchot

Rauytanapanit

Honda

et al. 2019

J Eukaryotic Microbiology

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Microalgae are a diverse group of photosynthetic eukaryotic organisms that are widely distributed globally. They are prolific sources of highly valuable compounds with fascinating chemical structures. Due to their balanced nutritional compositions and health benefits, they are increasingly being used as functional food ingredients. Carotenoid‐based pigments and polyunsaturated fatty acids (PUFAs) are examples of high‐value nutrients that can be accumulated abundantly in microalgae. Here, the effects of potassium chloride‐induced stress on the productions of lipids and carotenoids in the green microalga of the Chlorococcaceae family were investigated. Under normal BG11 medium, this green microalga strain TISTR 9500 accumulated high levels of PUFA and primary carotenoid lutein. Stress tests revealed that KCl enhanced and modulated lipid and carotenoid accumulation levels. The liquid chromatography‐tandem mass spectrometry (LC‐MS/MS) analysis revealed that secondary carotenoids astaxanthin and canthaxanthin were robustly produced under KCl stress with the similar content of lutein. Further, this stress led to a significant increase in the total FA amount with the higher proportion of unsaturated FA than saturated FA. Thus, this green microalga could be an attractive and alternative natural biosource for canthaxanthin and astaxanthin, as well as for functional lipids.

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“…; Bermejo et al. ; Blanco et al. ), our results reveal a rare case where a microalga can produce the β‐carotene family from this pathway.…”

Section: Discussionsupporting

confidence: 58%

Section: Discussioncontrasting

confidence: 54%

Effects of Potassium Chloride‐Induced Stress on the Carotenoids Canthaxanthin, Astaxanthin, and Lipid Accumulations in the Green Chlorococcal Microalga Strain TISTR 9500

Janchot

Rauytanapanit

Honda

et al. 2019

J Eukaryotic Microbiology

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“…The total lipid content in MZ–Ch64 is comparable with oleaginous strains C. onubensis – 48.7% of biomass dry weight, grown under continuous illumination (Bermejo et al. ) and C. subellipsoidea C‐169 (=NIES 2166) – 48.8%–50.5% of biomass dry weight, grown under complete N‐starvation conditions (Wang et al. ).…”

Section: Discussionmentioning

confidence: 75%

“…These values are higher than in the other oleaginous Coccomyxa strains like C. melkonianii SCCA 048 using BBM medium and nitrogen starvation conditions for cultivation (22.0%-23.0% and about 40% of biomass dry weight, respectively; Soru et al 2018). The total lipid content in MZ-Ch64 is comparable with oleaginous strains C. onubensis -48.7% of biomass dry weight, grown under continuous illumination (Bermejo et al 2018) and C. subellipsoidea C-169 (=NIES 2166) -48.8%-50.5% of biomass dry weight, grown under complete N-starvation conditions (Wang et al 2017).…”

Section: Controlmentioning

confidence: 74%

Fatty Acid Content and Profile of the Novel Strain of Coccomyxa elongata (Trebouxiophyceae, Chlorophyta) Cultivated at Reduced Nitrogen and Phosphorus Concentrations

Maltsev

Maltseva

et al. 2019

Journal of Phycology

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A novel freshwater strain of Coccomyxa elongata (MZ–Ch64) was isolated from the Zaporizhia region, Ukraine. The identification was based on the phylogenetic analysis of SSU rDNA gene and ITS1–5.8S rDNA–ITS2 region and predicted secondary structure of the ITS2. Phylogenetic analysis placed this strain in the Coccomyxa group, within the class Trebouxiophyceae. The novel strain MZ–Ch64 formed a strongly supported lineage closest with C. elongata. The MZ–Ch64 strain differed from the morphological description of the species by the size of vegetative cells and absence of small mucilaginous caps at one end of the cell. A number of experiments with different concentrations of phosphate and nitrate were conducted to evaluate changes in the resulting fatty acid profiles and biomass productivity. The fatty acid profile and total fatty acids varied significantly under different nutrient deficiencies. The dominant fatty acid during cultivation on standard BBM medium, as well as in phosphorus‐depleted conditions, was oleic acid (to 48.0%–54.6% of total fatty acids). Absence of nitrogen alone, and absence of both nitrogen and phosphorus, led to an increase of palmitic acid (to 24.7%–25.6%), cis‐7‐hexadecenoic acid (to 14.8%) and α‐linolenic acid (to 9.1%–10.1%) in comparison with the control sample. The greatest variation was found for oleic acid (31.9%–54.6%). Thus, this strain can be considered as a potential producer of oleic acid or cis‐7‐hexadecenoic and α‐linolenic acids for biotechnological applications.

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“…These down‐regulated acids mostly contribute to fatty acid biosynthesis metabolism. The release of fatty acids from membranes has also been found to be involved in plant tolerance to biotic and abiotic stresses (Xu et al ., 2011; Zhao et al ., 2016; Bermejo et al ., 2018; Kısa and Öztürk, 2019). Our results indicate that C. argentea can adapt to Cu stress by reducing fatty acid metabolism.…”

Section: Discussionmentioning

confidence: 99%

Analysis of physiological and metabolite response of Celosia argentea to copper stress

Wang

et al. 2021

Plant Biol J

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Copper‐tolerant (Cu) plants with high ornamental value play an important role in the ecological restoration of the copper tail mining area. We first discovered Celosia argentea adaptability in a copper mine area in China; however, its resistance to Cu and the underlying mechanism are not clear. In this study, C. argentea was selected for pot culture experiments. Its heavy metal accumulation and translocation, physiological and metabolic products were analysed under different growth concentrations of Cu (0–2400 mg.kg−1) stress. Our results indicated that roots strongly accumulated Cu2+. Oxidative stress defence mechanisms were activated in leaves under Cu treatment. Higher Cu concentrations triggered higher electrolyte leakage (EL), Malondialdehyde (MDA), superoxide dismutase (SOD) and peroxidase (POD) activity, and consequently a higher capacity to scavenge oxygen radicals and maintain cellular membrane integrity. In the citrate cycle, some amino acids and sugars related to biological pathways were altered in C. argentea exposed to Cu stress. Metabolomics data revealed that C. argentea used elevated sugar content as an antioxidant to regulate reactive oxygen species (ROS). Some organic acids and amino acids were up‐regulated compared with the control, indicating that these may chelate Cu in cells to remove excess Cu2+. The up‐regulation of polyamines and some organic acids may mitigate oxidative stress. These results indicate that C. argentea could be used as a Cu‐tolerant plant in Cu mine restoration. Its Cu tolerance mechanism also provides a basis for future plant improvement or breeding for use in mine restoration.

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Production of lutein, and polyunsaturated fatty acids by the acidophilic eukaryotic microalga Coccomyxa onubensis under abiotic stress by salt or ultraviolet light

Cited by 57 publications

References 49 publications

Effects of Potassium Chloride‐Induced Stress on the Carotenoids Canthaxanthin, Astaxanthin, and Lipid Accumulations in the Green Chlorococcal Microalga Strain TISTR 9500

Effects of Potassium Chloride‐Induced Stress on the Carotenoids Canthaxanthin, Astaxanthin, and Lipid Accumulations in the Green Chlorococcal Microalga Strain TISTR 9500

Fatty Acid Content and Profile of the Novel Strain of Coccomyxa elongata (Trebouxiophyceae, Chlorophyta) Cultivated at Reduced Nitrogen and Phosphorus Concentrations

Analysis of physiological and metabolite response of Celosia argentea to copper stress

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