Novel subunit vaccine with linear array epitope protect giant grouper against nervous necrosis virus infection

Lin, Chao Fen; Jiang, Han-Kai; Chen, Nai Chi; Wang, Ting Yu; Chen, Tzong Yueh

doi:10.1016/j.fsi.2018.01.029

Cited by 28 publications

(15 citation statements)

References 39 publications

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“…Another strategy for NNV vaccine development has been the use of subunit vaccines [163]. The latest studies include viral protein expression in recombinant yeast Sacharomyces cerevissae [333] or tobacco chloroplasts [334] as well as in Escherichia coli [335] and the use of a linear array epitope (LAE) technique [336]. The expression of the viral protein as virus-like particles (VLPs) has also been achieved in the yeast Yarrowia lipolytica [337].…”

Section: Preventionmentioning

confidence: 99%

“…Protection conferred by recombinant-protein vaccines has been reported to be high, i.e., vaccinated giant grouper RPS values above 72% [336] and increasing up to more than 80% in Atlantic sea bass [340]. Recently, total protection has been claimed in vaccinated convict/seven band grouper and European sea bass [335,341].…”

Section: Preventionmentioning

confidence: 99%

“…injection [289,328,329,331,332,349]. Injection has also been used for DNA vaccines [347,349] and subunit vaccines [335,336,338,[340][341][342] and oral immunization strategies have been used either for inactivated, subunit or DNA vaccines [225,[333][334][335]341,343,348,351,352]. The oral vaccine was given to the fish mixed with food [343] or by oral gavage [333,341] and encapsulated using either chemical compounds [225,348] or Artemia [353].…”

Section: Preventionmentioning

confidence: 99%

See 2 more Smart Citations

Betanodavirus and VER Disease: A 30-year Research Review

2020

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The outbreaks of viral encephalopathy and retinopathy (VER), caused by nervous necrosis virus (NNV), represent one of the main infectious threats for marine aquaculture worldwide. Since the first description of the disease at the end of the 1980s, a considerable amount of research has gone into understanding the mechanisms involved in fish infection, developing reliable diagnostic methods, and control measures, and several comprehensive reviews have been published to date. This review focuses on host-virus interaction and epidemiological aspects, comprising viral distribution and transmission as well as the continuously increasing host range (177 susceptible marine species and epizootic outbreaks reported in 62 of them), with special emphasis on genotypes and the effect of global warming on NNV infection, but also including the latest findings in the NNV life cycle and virulence as well as diagnostic methods and VER disease control.

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Section: Preventionmentioning

confidence: 99%

Section: Preventionmentioning

confidence: 99%

Section: Preventionmentioning

confidence: 99%

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Betanodavirus and VER Disease: A 30-year Research Review

2020

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“…For example, nervous necrosis virus (NNV) VLP vaccines that utilized E. coli , yeast, baculovirus, and plant or cell-free self-assembled expression were produced. Those studies showed that VLPs with similar size and geometry to the native virus could elicit an antibody response in fish by injection [103,104,105,106,107,108]. Chien et al and Cho et al developed oral VLP vaccines against grouper NNV [109,110].…”

Section: Alternative Vaccine Technologymentioning

confidence: 99%

A Review of Fish Vaccine Development Strategies: Conventional Methods and Modern Biotechnological Approaches

et al. 2019

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Fish immunization has been carried out for over 50 years and is generally accepted as an effective method for preventing a wide range of bacterial and viral diseases. Vaccination efforts contribute to environmental, social, and economic sustainability in global aquaculture. Most licensed fish vaccines have traditionally been inactivated microorganisms that were formulated with adjuvants and delivered through immersion or injection routes. Live vaccines are more efficacious, as they mimic natural pathogen infection and generate a strong antibody response, thus having a greater potential to be administered via oral or immersion routes. Modern vaccine technology has targeted specific pathogen components, and vaccines developed using such approaches may include subunit, or recombinant, DNA/RNA particle vaccines. These advanced technologies have been developed globally and appear to induce greater levels of immunity than traditional fish vaccines. Advanced technologies have shown great promise for the future of aquaculture vaccines and will provide health benefits and enhanced economic potential for producers. This review describes the use of conventional aquaculture vaccines and provides an overview of current molecular approaches and strategies that are promising for new aquaculture vaccine development.

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“…Vaccination is one of the most effective interventions and innovative strategies for research, and development of vaccines must be encouraged in aquaculture (Kai et al., 2014; MacLachlan & Dubovi, 2016). Although there is an effort to develop vaccines for grouper against NNV in Taiwan, commercial application is still not established due partly to the lack of a cheaper manufacturing process and effective protection (Lin, Jiang, Chen, Wang, & Chen, 2018). Hence, to provide insight into the development of NNV vaccination programme, we tended to apply the transmission dynamic models to evaluate the effectiveness of vaccination.…”

Section: Introductionmentioning

confidence: 99%

Modelling the effect of vaccination on transmission dynamics of nervous necrosis virus in grouper larvaeEpinephelus coioides

Chen

Yang

et al. 2020

Journal of Fish Diseases

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Nervous necrosis virus (NNV) infection in susceptible grouper larvae has been reported to cause high mortalities, leading to great economic losses in aquaculture industry. Although the effects of NNV vaccines on grouper have been broadly investigated, vaccination strategies have not been fully established. To this end, we introduced the parsimonious epidemiological models that explored the assessment of key epidemiological parameters and how they changed when vaccinations showed the effects. We showed that the models capture the published cumulative mortality data accurately. We estimated a basic reproduction number R 0 = 2.44 for NNV transmission in grouper larvae without vaccination. To effectively control NNV transmission by vaccination, a model for disease control was also generalized to attain the goals of controlled reproduction number less than 1. Our results indicated that at least 60% of grouper population needed to be immunized for ~75 min. Our data-driven modelling approach that links the transmission dynamics of NNV and vaccination strategies for grouper has the potential to support evidence-based planning and adaptation of integrated control measures. We encourage that the epidemiology-based framework introduced here can be further implemented for establishing effective vaccination and mitigation actions aimed at controlling diseases in fish farming practices.

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Novel subunit vaccine with linear array epitope protect giant grouper against nervous necrosis virus infection

Cited by 28 publications

References 39 publications

Betanodavirus and VER Disease: A 30-year Research Review

Betanodavirus and VER Disease: A 30-year Research Review

A Review of Fish Vaccine Development Strategies: Conventional Methods and Modern Biotechnological Approaches

Modelling the effect of vaccination on transmission dynamics of nervous necrosis virus in grouper larvaeEpinephelus coioides

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