Controlled synthesis and size effects of multifunctional mesoporous silica nanosystem for precise cancer therapy

Abstract

Nanomaterials-based drug delivery systems display potent applications in cancer therapy, owing to the enhanced permeability and retention effect and diversified chemical modification. In this study, we have tailored and synthesized different sized mesoporous silica nanoparticles (MSNs) through reactant control to investigate the relevancy of nanoparticle size toward anticancer efficacy and suppressing cancer multidrug resistance. The different sized MSNs loaded with anticancer ruthenium complex (RuPOP) and conjugated with folate acid (FA) to enhance the selectivity between cancer and normal cells. The nanosystem (Ru@MSNs) can specifically recognize HepG2 hepatocellular carcinoma cells, thus enhance accumulation and selective cellular uptake. The smaller sized (20 nm) Ru@MSNs exhibit higher anticancer activity against HepG2 cells, while the larger sized (80 nm) Ru@MSNs exhibit higher inhibitory effect against DOX-resistant hepatocellular carcinoma cells (R-HepG2). Moreover, Ru@MSNs induced ROS overproduction in cancer cells, leading to DNA damage and p53 phosphorylation, consequently promoting cancer cells apoptosis. Ru@MSNs (80 nm) also inhibited ABCB1 and ABCG2 expression in R-HepG2 cells to prevent drug efflux, thus overcome multidrug resistance. Ru@MSNs also inhibited tumor growth in vivo without obvious toxicity in major organs of tumor-bearing nude mice. Taken together, these results verify the size effects of MSNs nanosystem for precise cancer therapy.

Tea regimen, a comprehensive assessment of antioxidant and antitumor activities of tea extract produced by Tie Guanyin hybridization

“…In order to further study the anti-cancer effect of STE in vivo, we constructed the MDA-MB-231 in vivo xenogra model, the specic experimental methods were described as previously reported. 34,35 Briey, the in vivo antitumor activity of STE was measured aer 24 days' daily irrigation of the nude mice. The blood sample (72 h) of the nude mice was subjected to haematological analysis at Guangzhou Overseas Chinese Hospital.…”

Cancer-targeted design of bioresponsive prodrug with enhanced cellular uptake to achieve precise cancer therapy

“…After which the cells were subjected to 1 μM biotin-conjugated CPTs at 37 °C for 2 h in the dark. At intervals of 10 min from 0 min to 60 min, the intracellular ROS level was measured by fluorescence microplate reader (CYTATION5, Biotek) to detect the intensity of DCF fluorescence (excitation and emission wavelength at 488 and 525 nm) (Ma et al., 2018 ). For DHE (red) probe, the cells were treated as same as DCF and finally subjected to a microscope (EVOSs FL Auto Imaging System: Life Technologies, AMAFD1000 (Thermo Fisher Scientific), Beijing, China) for observation and photographing at intervals of 30 min from 0 to 60 min.…”

Design and Synthesis of 2‐(5‐Phenylindol‐3‐yl)benzimidazole Derivatives with Antiproliferative Effects towards Triple‐Negative Breast Cancer Cells by Activation of ROS‐Mediated Mitochondria Dysfunction

“…The experiment was conducted according to al iterature method. [26] DCFH-DA was used to detect intracellular ROS levels. Briefly,M DA-MB-231 cells were seeded at 2 10 4 cells per well in 96-well tissue culture plates and incubated at 37 8Cf or 24 h. The cells were incubated with DCFH-DA (10 mm)f or 30 min at 37 8C, then the cells were washed three times with PBS, then 1b or 2a with the same concentration gradient was immediately added to the plates.…”