Long non‐coding RNA TCONS_00041960 enhances osteogenesis and inhibits adipogenesis of rat bone marrow mesenchymal stem cell by targeting miR‐204‐5p and miR‐125a‐3p

Shang, Guowei; Wang, Yadong; Xu, Yan; Zhang, Shanfeng; Sun, Xiaoya; Guan, Hongya; Zhao, Xuefeng; Wang, Yisheng; Li, Yuebai; Zhao, Guoqiang

doi:10.1002/jcp.26424

Cited by 61 publications

(47 citation statements)

References 38 publications

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“…To date, many lncRNAs have been demonstrated to regulate osteogenic differentiation, including lncRNA MEG3, lncRNA TCONS_00041960, H19, and so on (B. Li et al, 2017Li et al, , 2018Liao et al, 2017;Shang et al, 2018;Wu et al, 2018;Q. Wang et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

A novel long noncoding RNA AK016739 inhibits osteoblast differentiation and bone formation

Chen

Wang

et al. 2019

Journal Cellular Physiology

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The incidence of postmenopausal osteoporosis research 50% in middle‐aged and older women, however, effects of existing therapy are not ideal. Emerging evidence have proved that long noncoding RNAs (lncRNAs) was correlated with multiple physiological and pathology processes including development, carcinogenesis, and osteogenesis. However, reports on lncRNAs regulating bone formation were relatively limited. In this study, we screened osteogenic lncRNAs through mRNA/lncRNA microarray combined with gene coexpression analysis. The biological function of the screened lncRNA was assessed both in vitro and in vivo. The effects of the lncRNA on osteogenic transcription factors were also evaluated. We identified AK016739, which was correlated with osteogenic differentiation and enriched in skeletal tissues of mice. The expression levels of AK016739 in bone‐derived mesenchymal stem cells were increased with age and negatively correlated with osteogenic differentiation marker genes. Experiments showed that AK016739 inhibited osteoblast differentiation, and in vivo inhibition of AK016739 by its small interfering RNA would rescue bone formation in ovariectomized osteoporosis mice model. In addition, AK016739 suppressed both expression levels and activities of osteogenic transcription factors. This newly identified lncRNA AK016739 has revealed a new mechanism of osteogenic differentiation and provided new targets for treatment of skeletal disorders.

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Section: Discussionmentioning

confidence: 99%

A novel long noncoding RNA AK016739 inhibits osteoblast differentiation and bone formation

Chen

Wang

et al. 2019

Journal Cellular Physiology

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“…Being one of the important classes of noncoding RNAs, the role of numerous lncRNAs has been identified in MSCs. For instance, lncRNA TCONS_00041960 [24] can target miR-204-5p and miR-125a-3p to enhance osteogenesis and inhibit adipogenesis. lncRNA-p21 [25], under hypoxic preconditioning, can be a positive factors for MSC migration and survival capacity.…”

Section: Discussionmentioning

confidence: 99%

lncRNA-TINCR Functions as a Competitive Endogenous RNA to Regulate the Migration of Mesenchymal Stem Cells by Sponging miR-761

Zheng

Huang

Liu

et al. 2020

BioMed Research International

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Mounting evidences have indicated that terminal differentiation-induced lncRNA (TINCR) contributes to various cellular processes, such as proliferation, apoptosis, autophagy, migration, invasion, and metastasis. However, the function of TINCR in regulating migration of MSCs is largely unknown. In this study, the effects of TINCR on the migration of rat MSCs from the bone marrow were studied by Transwell assays and wound healing assays. Our results suggested that TINCR positively regulated migration of rMSCs. miR-761 mimics suppressed rMSC migration, whereas miR-761 inhibitor promoted migration. Target prediction analysis tools and dual-luciferase reporter gene assay identified Wnt2 as a direct target of miR-761. miR-761 could inhibit the expression of Wnt2. Further, the investigation about the function of TINCR in miR-761-induced migration of rMSCs was completed. These results demonstrated that TINCR took part in the regulation of miR-761-induced migration in rMSCs through the regulation of Wnt2 and its Wnt2 signaling pathway. Taken together, our results demonstrate that lncRNA-TINCR functions as a competitive endogenous RNA (ceRNA) to regulate the migration of rMSCs by sponging miR-761 which modulates the role of Wnt2. These findings provide evidence that lncRNA-TINCR has a chance to serve as a potential target for enhancing MSC homing through the miR-761/Wnt2 signaling pathway.

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“…Further, Zhao et al [33] provided evidence that lncRNA-plasmacytoma variant translocation 1 functions as an endogenous 'sponge' by competing for miR-448 binding to regulate expression of the target SERBP1, thereby promoting the proliferation and migration of pancreatic cancer cells. Finally, Shang et al found that lncRNA TCONS_00041960 enhances osteogenesis and inhibits adipogenesis in rat bone marrow mesenchymal stem cells by targeting miR-204-5p and miR-125a-3p [34].…”

Section: Discussionmentioning

confidence: 99%

Relationship among porcine lncRNA TCONS_00010987, miR-323, and leptin receptor based on dual luciferase reporter gene assays and expression patterns

Ding

Wang

et al. 2020

Asian-Australas J Anim Sci

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Considering the physiological and clinical importance of leptin receptor (LEPR) in regulating obesity and the fact that porcine LEPR expression is not known to be controlled by lncRNAs and miRNAs, we aim to characterize this gene as a potential target of SSC-miR-323 and the lncRNA TCONS_00010987. Methods: Bioinformatics analyses revealed that lncRNA TCONS_00010987 and LEPR have SSC-miR-323-binding sites and that LEPR might be a target of lncRNA TCONS_00010987 based on cis prediction. Wild-type and mutant TCONS_00010987-target sequence fragments and wild-type and mutant LEPR 3′-UTR fragments were generated and cloned into pmiR-RB-REPORT TM-Control vectors to construct respective recombinant plasmids. HEK293T cells were co-transfected with the SSC-miR-323 mimics or a negative control with constructs harboring the corresponding binding sites and relative luciferase activities were determined. Tissue expression patterns of lncRNA TCONS_00010987, SSC-miR-323, and LEPR in Anqing six-end-white (AQ, the obese breed) and Large White (LW, the lean breed) pigs were detected by real-time quantitative polymerase chain reaction; backfat expression of LEPR protein was detected by western blotting. Results: Target gene fragments were successfully cloned, and the four recombinant vectors were constructed. Compared to the negative control, SSC-miR-323 mimics significantly inhibited luciferase activity from the wild-type TCONS_00010987-target sequence and wildtype LEPR-3′-UTR (p<0.01 for both) but not from the mutant TCONS_00010987-target sequence and mutant LEPR-3′-UTR (p>0.05 for both). Backfat expression levels of TCONS_ 00010987 and LEPR in AQ pigs were significantly higher than those in LW pigs (p<0.01), whereas levels of SSC-miR-323 in AQ pigs were significantly lower than those in LW pigs (p<0.05). LEPR protein levels in the backfat tissues of AQ pigs were markedly higher than those in LW pigs (p<0.01). Conclusion: LEPR is a potential target of SSC-miR-323, and TCONS_00010987 might act as a sponge for SSC-miR-323 to regulate LEPR expression.

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Long non‐coding RNA TCONS_00041960 enhances osteogenesis and inhibits adipogenesis of rat bone marrow mesenchymal stem cell by targeting miR‐204‐5p and miR‐125a‐3p

Cited by 61 publications

References 38 publications

A novel long noncoding RNA AK016739 inhibits osteoblast differentiation and bone formation

A novel long noncoding RNA AK016739 inhibits osteoblast differentiation and bone formation

lncRNA-TINCR Functions as a Competitive Endogenous RNA to Regulate the Migration of Mesenchymal Stem Cells by Sponging miR-761

Relationship among porcine lncRNA TCONS_00010987, miR-323, and leptin receptor based on dual luciferase reporter gene assays and expression patterns

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