Precise Cerebral Vascular Atlas in Stereotaxic Coordinates of Whole Mouse Brain

Xiong, Binbin; Li, Anan; Yang, Lei; Chen, Shangbin; Long, Ben; Peng, Jinggang; Yang, Zhongqin; Xu, Tonghui; Yang, Xiaoquan; Li, Xiangning; Jiang, Tao; Luo, Qingming; Gong, Hui

doi:10.3389/fnana.2017.00128

Cited by 197 publications

(206 citation statements)

References 53 publications

Supporting

Mentioning

188

Contrasting

Order By: Relevance

“…We concluded that the labeled vascular volume accounts for 2 ± 1% (mean ± standard deviation) in the imaged column of the mouse cortex (~2mm lateral and 2mm caudal to the Bregma point, Figure 2B). Our result is in close agreement with other studies quantifying the fractional vascular volume by imaging sliced brains ex vivo (~2% for blood vessels of 20 μm or less diameter in the primary somatosensory cortex) (Wang et al, 2019; Xiong et al, 2017). We noticed that most of the blood vessels in the imaged volume has a diameter of less than 20 μm, except for a few on the brain surface (Figure 2-figure supplement 1A).…”

Section: Methodssupporting

confidence: 93%

Quantitative Analysis of 1300-nm Three-photon Calcium Imaging in the Mouse Brain

Wang

Ouzounov

et al. 2019

Preprint

View full text Add to dashboard Cite

1300-nm three-photon calcium imaging has emerged as a useful technique to allow calcium imaging in deep brain regions. Application to large-scale neural activity imaging entails a careful balance between recording fidelity and tissue heating. We calculated and experimentally verified the excitation pulse energy to achieve the minimum photon count required for the detection of calcium transients in GCaMP6s-expressing neurons for 920-nm two-photon and 1320-nm three-photon excitation, respectively. Brain tissue heating by continuous three-photon imaging was simulated with Monte Carlo method and experimentally validated with immunohistochemistry. We observed increased immunoreactivity with 150 mW excitation power at 1.0- and 1.2-mm imaging depths. Based on the data, we explained how three-photon excitation achieves better calcium imaging fidelity than two-photon excitation in the deep brain and quantified the imaging depth where three-photon microscopy should be applied. Our analysis presents a translatable model for the optimization of three-photon calcium imaging based on experimentally tractable parameters.

show abstract

Section: Methodssupporting

confidence: 93%

Quantitative Analysis of 1300-nm Three-photon Calcium Imaging in the Mouse Brain

Wang

Ouzounov

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…Fixed brain tissue was cut into 3 sets of 30-μm-thick coronal sections using a freezing microtome. Coronal brain sections including the rPOA and organum vasculosum of lamina terminalis (OVLT) of the hypothalamus were selected according to the mouse brain atlas (75). Free-floating immunohistochemistry was performed as previously described (26,33) on one set of sections (including every third brain section).…”

Section: Methodsmentioning

confidence: 99%

Ontogeny and reversal of brain circuit abnormalities in a preclinical model of PCOS

Silva¹,

Prescott²,

Campbell³

2018

JCI Insight

View full text Add to dashboard Cite

Androgen excess is a hallmark of polycystic ovary syndrome (PCOS), a prevalent yet poorly understood endocrine disorder. Evidence from women and preclinical animal models suggests that elevated perinatal androgens can elicit PCOS onset in adulthood, implying androgen actions in both PCOS ontogeny and adult pathophysiology. Prenatally androgenized (PNA) mice exhibit a robust increase of progesterone-sensitive GABAergic inputs to gonadotropin-releasing hormone (GnRH) neurons implicated in the pathogenesis of PCOS. It is unclear when altered GABAergic wiring develops in the brain, and whether these central abnormalities are dependent upon adult androgen excess. Using GnRH-GFP-transgenic mice, we determined that increased GABA input to GnRH neurons occurs prior to androgen excess and the manifestation of reproductive impairments in PNA mice. These data suggest that brain circuit abnormalities precede the postpubertal development of PCOS traits. Despite the apparent developmental programming of circuit abnormalities, long-term blockade of androgen receptor signaling from early adulthood rescued normal GABAergic wiring onto GnRH neurons, improved ovarian morphology, and restored reproductive cycles in PNA mice. Therefore, androgen excess maintains changes in female brain wiring linked to PCOS features and the blockade of androgen receptor signaling reverses both the central and peripheral PNA-induced PCOS phenotype.

show abstract

“…However, such methods including local spatial regularization cannot segment large vascular networks across changing intensity distributions. Finally, the size of the acquired datasets poses a difficulty to assess the organization of the whole vascular network; therefore, such methods can only segment small volumes [15][16][17][18][19] .…”

Section: Introductionmentioning

confidence: 99%

Automated analysis of whole brain vasculature using machine learning

Schoppe

et al. 2019

Preprint

View full text Add to dashboard Cite

Tissue clearing methods enable imaging of intact biological specimens without sectioning. However, reliable and scalable analysis of such large imaging data in 3D remains a challenge. Towards this goal, we developed a deep learning-based framework to quantify and analyze the brain vasculature, named Vessel Segmentation & Analysis Pipeline (VesSAP). Our pipeline uses a fully convolutional network with a transfer learning approach for segmentation. We systematically analyzed vascular features of the whole brains including their length, bifurcation points and radius at the micrometer scale by registering them to the Allen mouse brain atlas. We reported the first evidence of secondary intracranial collateral vascularization in CD1-Elite mice and found reduced vascularization in the brainstem as compared to the cerebrum. VesSAP thus enables unbiased and scalable quantifications for the angioarchitecture of the cleared intact mouse brain and yields new biological insights related to the vascular brain function.

show abstract

Precise Cerebral Vascular Atlas in Stereotaxic Coordinates of Whole Mouse Brain

Cited by 197 publications

References 53 publications

Quantitative Analysis of 1300-nm Three-photon Calcium Imaging in the Mouse Brain

Quantitative Analysis of 1300-nm Three-photon Calcium Imaging in the Mouse Brain

Ontogeny and reversal of brain circuit abnormalities in a preclinical model of PCOS

Automated analysis of whole brain vasculature using machine learning

Contact Info

Product

Resources

About