The Effects of Platelet-Rich Plasma and Adipose-Derived Stem Cells on Neovascularization and Fat Graft Survival

Xiong, Bingjun; Tan, Qiuwen; Chen, Yujuan; Zhang, Yi; Zhang, Di; Tang, Shenli; Zhang, Shu; Lv, Qing

doi:10.1007/s00266-017-1062-1

Cited by 34 publications

(31 citation statements)

References 31 publications

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“…However, only a few studies report the effects of different PRP concentrations on ADSC proliferation. Early studies found that PRP had a dose dependent effect on proliferation of ADSCs[16-18]. However, later studies had opposite opinions.…”

Section: Discussionmentioning

confidence: 99%

Platelet-rich plasma enhances adipose-derived stem cell-mediated angiogenesis in a mouse ischemic hindlimb model

Chen

Liao

2018

WJSC

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AIMTo evaluate the angiogenic effect of platelet-rich plasma (PRP)-preconditioned adipose-derived stem cells (ADSCs) both in vitro and in a mouse ischemic hindlimb model.METHODSADSCs were divided based on culture medium: 2.5% PRP, 5% PRP, 7.5% PRP, and 10% PRP. Cell proliferation rate was analyzed using the MTS assay. The gene expression of CD31, vascular endothelial growth factor, hypoxia-inducible factors, and endothelial cell nitric oxide synthase was analyzed using reverse transcription polymerase chain reaction. Cell markers and structural changes were assessed through immunofluorescence staining and the tube formation assay. Subsequently, we studied the in vivo angiogenic capabilities of ADSCs by a mouse ischemic hindlimb model.RESULTSThe proliferation rate of ADSCs was higher in the 2.5%, 5%, and 7.5% PRP groups. The expression of hypoxia-inducible factor, CD31, vascular endothelial growth factor, and endothelial cell nitric oxide synthase in the 5% and 7.5% PRP groups increased. The 5%, 7.5%, and 10% PRP groups showed higher abilities to promote both CD31 and vascular endothelial growth factor production and tubular structure formation in ADSCs. According to laser Doppler perfusion scan, the perfusion ratios of ischemic limb to normal limb were significantly higher in 5% PRP, 7.5% PRP, and human umbilical vein endothelial cells groups compared with the negative control and fetal bovine serum (FBS) groups (0.88 ± 0.08, 0.85 ± 0.07 and 0.81 ± 0.06 for 5%, 7.5% PRP and human umbilical vein endothelial cells compared with 0.42 ± 0.17 and 0.54 ± 0.14 for the negative control and FBS, P < 0.01).CONCLUSIONPRP-preconditioned ADSCs presented endothelial cell characteristics in vitro and significantly improved neovascularization in ischemic hindlimbs. The optimal angiogenic effect occurred in 5% PRP- and 7.5% PRP-preconditioned ADSCs.

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Section: Discussionmentioning

confidence: 99%

Platelet-rich plasma enhances adipose-derived stem cell-mediated angiogenesis in a mouse ischemic hindlimb model

Chen

Liao

2018

WJSC

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“…ADSCs are defined as a multi‐lineage stem cell population, which are isolated from the stromal vascular fraction (SVF) of adipose tissues, and can be further induced to undergo differentiation into different cells based on lineage‐specific induction factors (Bianco et al, ; Bielli et al, ). ADSC populations were featured with antigenic and neovasculogenic properties, which are thus therapeutically attractive and advantageous to widely use as seeding cells for tissue engineering and regenerative medicine (Xiong et al, ). Recently, accumulating evidence has strongly implied that several cytokines are essential to improve the survival rate of vascular endothelial cells (VECs).…”

Section: Introductionmentioning

confidence: 99%

Effects of VEGF‐ANG‐1‐PLA nano‐sustained release microspheres on proliferation and differentiation of ADSCs

Chen

et al. 2018

Cell Biology International

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The improvement of fat graft viability might depend on the presence of multipotent resident adipose derived stem cells (ADSCs) which is the important component of stromal vascular fraction (SVF). Vascular endothelial growth factor (VEGF) and angiogenin-1 (Ang-1) are responsible for neovascularization. However, their half-life is too short to produce a biological effect. We thus investigated whether VEGF-ANG-1-polylactic acid (PLA) microspheres could enhance the angiogenic properties of ADSCs. PLA microspheres containing VEGF and ANG-1 were prepared by in vitro ultrasonic emulsification and characterized according to their encapsulation efficiency (EE), drug-loading rate (DL), particle size, and drug release. The systemic toxicity of empty loaded nanospheres (NPs) and the ability of these microspheres to promote the proliferation and differentiation of ADSCs were evaluated. The EE and DL were above 86 and 2.8%, respectively. The drug release was completed after 20 days. Systemic toxicity was verified in ADSCs that received the unloaded NPs. It was observed that ADSCs treated with VEGF-ANG-1-PLA microspheres had an increase in the proliferation and the number of CD31 positive cells. ADSCs proliferation and differentiation toward endothelial cells (ECs) could be enhanced by the addition of VEGF-ANG-1-PLA nano-sustained release microspheres.

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“…Further recent studies have shown that adding PRP to a lipofilling significantly improves neovascularization and long-term fat survival. 20,21 Seyhan et al 22 explored the use of PRP with added adipose-derived stem cells in rats transplanted with fat tissue and reported that this combination enhances the survival of transplanted fat tissue. Shahzad and Mehrara 23 speculated that PRP would greatly improve fat graft survival, yet reported mixed results, some studies showing improved fat retention with PRP and others showing no such benefit.…”

Section: Discussionmentioning

confidence: 99%

Platelet-rich plasma to rescue an ulcerated orbital dermal fat graft

Tapias

Díaz

Secondi

et al. 2018

European Journal of Ophthalmology

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Introduction: Central graft ulceration is a rare complication of an orbital dermal fat graft caused by diminished blood supply to the implant. This study reports on the efficacy and safety of the use of a single subconjunctival injection of autologous platelet-rich plasma to rescue an ulcerated orbital dermal fat graft. Methods: Three patients, who had undergone autologous dermal fat graft to treat their anophthalmic socket, were given a 2-mL subconjunctival platelet-rich plasma injection in the exposed graft margins. Demographic and clinical features, treatment protocol, clinical course, complications and follow-up time are reported. In the preoperative examination, all the patients presented a primary epithelial defect of the dermal fat graft. Results: There were no major complications such as necrosis or infection. One patient presented a small conjunctival granuloma at 1-month follow up. Mean postoperative follow-up duration was 13 (range: 10–16) months. By 1 month, the chronic epithelial defect had resolved with the graft integrated within the orbital tissues in all cases. All patients were referred for artificial prosthesis placement. Conclusion: Although further work is needed, our findings suggest that a single subconjunctival platelet-rich plasma injection could be an effective, safe and economic alternative to surgery to rescue an ulcerated orbital dermal fat graft.

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The Effects of Platelet-Rich Plasma and Adipose-Derived Stem Cells on Neovascularization and Fat Graft Survival

Abstract: This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .

Cited by 34 publications

References 31 publications

Platelet-rich plasma enhances adipose-derived stem cell-mediated angiogenesis in a mouse ischemic hindlimb model

Platelet-rich plasma enhances adipose-derived stem cell-mediated angiogenesis in a mouse ischemic hindlimb model

Effects of VEGF‐ANG‐1‐PLA nano‐sustained release microspheres on proliferation and differentiation of ADSCs

Platelet-rich plasma to rescue an ulcerated orbital dermal fat graft

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