2018
DOI: 10.1042/bsr20171664
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LncRNA DANCR functions as a competing endogenous RNA to regulate RAB1A expression by sponging miR-634 in glioma

Abstract: Long noncoding RNA (lncRNA) differentiation antagonizing nonprotein coding RNA (DANCR) plays important regulatory roles in many solid tumors. However, the effect of DANCR in glioma progression and underlying molecular mechanisms were not entirely explored. In the present study, we determined the expression of DANCR in glioma tissues and cell lines using qRT-PCR and further defined the biological functions. Furthermore, we used luciferase reporter assay, Western blot, and RNA immunoprecipitation (RIP) to explor… Show more

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Cited by 44 publications
(39 citation statements)
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“…Indeed, as shown in Figure 4B, DANCR with wild-type 3'UTR sequence (wt) interacted with miR-874-3P, and DANCR with mutations (mut) in miR-874-3P targeting sites had no similar effect. As positive controls, miR-634 and miR-496, two miRNAs previously shown to bind with DANCR Xu et al, 2018), were simultaneously found present in pull-down products, confirming the validity of this assay. Moreover, the specific interaction between DANCR and miR-874-3P was also supported by the evidence obtained from anti-Ago2 RNA immunoprecipitation (RIP) assay (Figure 4C).…”
Section: Dancr Directly Targets Mir-874-3p In Aml Cellssupporting
confidence: 74%
See 1 more Smart Citation
“…Indeed, as shown in Figure 4B, DANCR with wild-type 3'UTR sequence (wt) interacted with miR-874-3P, and DANCR with mutations (mut) in miR-874-3P targeting sites had no similar effect. As positive controls, miR-634 and miR-496, two miRNAs previously shown to bind with DANCR Xu et al, 2018), were simultaneously found present in pull-down products, confirming the validity of this assay. Moreover, the specific interaction between DANCR and miR-874-3P was also supported by the evidence obtained from anti-Ago2 RNA immunoprecipitation (RIP) assay (Figure 4C).…”
Section: Dancr Directly Targets Mir-874-3p In Aml Cellssupporting
confidence: 74%
“…One critical mechanism by which lncRNAs regulate gene expression is acting as ceRNAs to sponge targeted miRNAs (Salmena et al, 2011). This is also true for DANCR, not only because several miRNAs have been identified as direct targets of DANCR, but also the function of DANCR is prominently mediated through downregulating the expression of these miRNAs, as reported by recent studies Lu et al, 2018;Wang et al, 2018a;Wang et al, 2018b;Xu et al, 2018;Yang et al, 2018), to name a few. Combining predictive methods and some biochemical experiments, we demonstrate that miR-874-3P is a new binding target of DANCR and that by suppressing the inhibitory effect of miR-874-3P on ATG16L1 expression via interacting and decreasing miR-874-3P level, DANCR possesses an ability to upregulate ATG16L1 expression.…”
Section: Disscussionmentioning
confidence: 89%
“…Mounting evidence suggested that lncRNAs could function as miRNA sponges or decoys to regulate the expressions of miRNAs targets genes in different types of cancer, including glioma [26,27]. Some literatures have revealed several target miRNAs of DANCR in glioma.…”
Section: Discussionmentioning
confidence: 99%
“…Some literatures have revealed several target miRNAs of DANCR in glioma. For instance, DANCR regulated RAB1A expression in glioma by functioning as a ceRNA of miR-634 [27]. In addition, DANCR has been reported to promote glioma malignancy by miR-33a-5p [28].…”
Section: Discussionmentioning
confidence: 99%
“…[12][13][14] Moreover, lncRNA DANCR is also suggested in some studies to be correlated with different tumor biological parameters, such as tumor growth, metastasis, and progression. [15][16][17] Metastasis and prognosis may be affected by lncRNA DANCR; nonetheless, a majority of existing studies are limited by their small sample sizes and discrete outcomes. As a consequence, an updated meta-analysis was performed in this study to determine the prognostic value of lncRNA DANCR in cancer patients.…”
Section: Introductionmentioning
confidence: 99%