In-vitro analysis of Quantum Molecular Resonance effects on human mesenchymal stromal cells

Sella, Sabrina; Adami, Valentina; Amati, Eliana; Bernardi, M.; Chieregato, Katia; Gatto, Pamela; Menarin, Martina; Pozzato, Alessandro; Pozzato, Gianantonio; Astori, Giuseppe

doi:10.1371/journal.pone.0190082

Cited by 20 publications

(26 citation statements)

References 50 publications

(47 reference statements)

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“…45,46 This technology creates quanta of energy able to break the molecular bonds at the tip of the needles without increasing the kinetic energy of the hit molecules; thus, without raising temperature, thus limiting the damage to the surrounding tissue. 47…”

Section: Introductionmentioning

confidence: 99%

Successful muscle regeneration by a homologous microperforated scaffold seeded with autologous mesenchymal stromal cells in a porcine esophageal substitution model

Marzaro

Algeri

Tomao

et al. 2020

Therap Adv Gastroenterol

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Background: Since the esophagus has no redundancy, congenital and acquired esophageal diseases often require esophageal substitution, with complicated surgery and intestinal or gastric transposition. Peri-and-post-operative complications are frequent, with major problems related to the food transit and reflux. During the last years tissue engineering products became an interesting therapeutic alternative for esophageal replacement, since they could mimic the organ structure and potentially help to restore the native functions and physiology. The use of acellular matrices pre-seeded with cells showed promising results for esophageal replacement approaches, but cell homing and adhesion to the scaffold remain an important issue and were investigated. Methods: A porcine esophageal substitute constituted of a decellularized scaffold seeded with autologous bone marrow-derived mesenchymal stromal cells (BM-MSCs) was developed. In order to improve cell seeding and distribution throughout the scaffolds, they were micro-perforated by Quantum Molecular Resonance (QMR) technology (Telea Electronic Engineering). Results: The treatment created a microporous network and cells were able to colonize both outer and inner layers of the scaffolds. Non seeded (NSS) and BM-MSCs seeded scaffolds (SS) were implanted on the thoracic esophagus of 4 and 8 pigs respectively, substituting only the muscle layer in a mucosal sparing technique. After 3 months from surgery, we observed an esophageal substenosis in 2/4 NSS pigs and in 6/8 SS pigs and a non-practicable stricture in 1/4 NSS pigs and 2/8 SS pigs. All the animals exhibited a normal weight increase, except one case in the SS group. Actin and desmin staining of the post-implant scaffolds evidenced the regeneration of a muscular layer from one anastomosis to another in the SS group but not in the NSS one. Conclusions: A muscle esophageal substitute starting from a porcine scaffold was developed and it was fully repopulated by BM-MSCs after seeding. The substitute was able to recapitulate in shape and function the original esophageal muscle layer.

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Section: Introductionmentioning

confidence: 99%

Successful muscle regeneration by a homologous microperforated scaffold seeded with autologous mesenchymal stromal cells in a porcine esophageal substitution model

Marzaro

Algeri

Tomao

et al. 2020

Therap Adv Gastroenterol

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“…ARHGAP22 displays medium-level expression in both the placenta and heart myocytes. It is involved in angiogenesis and regulates the actin cytoskeleton by suppressing Ras-related C3 botulinum toxin substrate 1 (Rac1) 27,28 , which suggests that altered gene expression could plausibly have a role in cardiac development and defects. Further in-vitro studies will be needed to characterize better this association.…”

Section: Discussionmentioning

confidence: 99%

Placental DNA methylation changes in detection of tetralogy of Fallot

Bahado‐Singh

Vishweswaraiah

Mishra

et al. 2020

Ultrasound in Obstet & Gyne

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Objectives To determine whether the methylation level of cytosine nucleotides in placental DNA can be used to predict tetralogy of Fallot (TOF) and provide insights into the developmental mechanism of this condition. Methods Tissue sections were obtained from formalin‐fixed paraffin‐embedded specimens of placental tissue obtained at birth from eight cases with non‐chromosomal, non‐syndromic TOF and 10 unaffected newborns. The Illumina Infinium HumanMethylation450 BeadChip assay was used to measure cytosine (‘CpG’ or ‘cg’) methylation levels at loci throughout the placental genome. Differential methylation was assessed by comparing the β‐values (a measure of the extent of cytosine methylation) for individual CpG loci in fetuses with TOF vs in controls. The most discriminating CpG sites were determined based on a preset cut‐off of ≥ 2.0‐fold change in the methylation level. The predictive accuracy of CpG loci with significant methylation changes for TOF was determined by the area under the receiver‐operating‐characteristics curve (AUC). A false‐discovery‐rate (FDR) P‐value < 0.05 was used to define a statistically significant difference in the methylation level. Ingenuity Pathway Analysis (IPA) (Qiagen) was used to identify gene pathways that were significantly overexpressed, and thus altered, in TOF cases compared with controls. Results We found a total of 165 significantly differentially methylated CpG loci in TOF cases compared with controls, in 165 separate genes. These biomarkers demonstrated from fair to excellent individual predictive accuracy for TOF detection, with AUCs ≥ 0.75 (FDR P‐value < 0.001 for all). The following CpG loci (gene) had the highest predictive accuracy: cg05273049 (ARHGAP22; AUC = 1.00; 95% CI, 1.00–1.00), cg02540011 (CDK5; AUC = 0.96; 95% CI, 0.87–1.00), cg08404201 (TRIM27; AUC = 0.95; 95% CI, 0.84–1.00) and cg00687252 (IER3; AUC = 0.95; 95% CI, 0.84–1.00). IPA revealed over‐representation (dysregulation) of 14 gene pathways involved in normal cardiac development, including cardiomyocyte differentiation via bone morphogenetic protein receptors, cardiac hypertrophy signaling and role of nuclear factor of activated T cells in cardiac hypertrophy. Cardiac hypertrophy is an important feature of TOF. Conclusions Analysis of placental DNA cytosine methylation changes yielded accurate markers for TOF detection and provided mechanistic information on TOF development. Our work appears to confirm the central role of epigenetic changes and of the placenta in the development of TOF. Copyright © 2019 ISUOG. Published by John Wiley & Sons Ltd.

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“…Researchers reported the up-regulation of vascular endothelial growth factor expression in chronic wounds treated by QMR technology [10]. There was also a study that suggested the QMR stimulation could trigger angiogenesis and tissue regeneration of human mesenchymal stromal cells [11].…”

Section: Discussionmentioning

confidence: 99%

Novel quantum molecular resonance energy source for laparoscopic bipolar vessel sealer: An experimental study in animal model

Kwon

et al. 2020

Preprint

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Background: The majority of conventional bipolar energy-based vessel sealing devices utilize energy at frequencies between 300 kHz and 500 kHz. The use of such frequencies has the disadvantages including unintended damage to surrounding tissues and excessive surgical smoke production. Here, we developed an bipolar energy source using Quantum Molecular Resonance (QMR) energy of 4–64 MHz and combined this into a laparoscopic vessel sealer. We investigate the microscopic tissue effect and surgeon's experiences of laparoscopic bipolar vessel sealer using a novel QMR energy source through animal experiments. Results: In an open surgical setting, QMR energy sources showed higher sealing success rates (100% vs. 66.7%) and higher burst pressure (963 mmHg vs. 802mmHg) of the sealed vessels compared to LigaSure™. Histological analysis showed less vessel wall injury in the QMR energy source (55.0% vs. 73.9%). In the laparoscopic setting experiments, compared to LigaSure™, QMR energy sources showed statistically significantly less smoke formation (p = 0.014), less tissue carbonization (p = 0.013), and less stickiness (p = 0.044) during sealing tissues. Conclusions: Novel QMR energy source for laparoscopic bipolar vessel sealer could produce better sealing performance and less surrounding tissue damage compared to the conventional devices. Laparoscopic surgery using QMR energy sources showed better surgeon's experiences in terms of surgical smoke formation, tissue carbonization, and stickiness.

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In-vitro analysis of Quantum Molecular Resonance effects on human mesenchymal stromal cells

Cited by 20 publications

References 50 publications

Successful muscle regeneration by a homologous microperforated scaffold seeded with autologous mesenchymal stromal cells in a porcine esophageal substitution model

Successful muscle regeneration by a homologous microperforated scaffold seeded with autologous mesenchymal stromal cells in a porcine esophageal substitution model

Placental DNA methylation changes in detection of tetralogy of Fallot

Novel quantum molecular resonance energy source for laparoscopic bipolar vessel sealer: An experimental study in animal model

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