Wound healing-related properties detected in an experimental model with a collagen gel contraction assay are affected in the absence of tenascin-X

Hashimoto, Kei; Kajitani, Naoto; Miyamoto, Yasunori; Matsumoto, K.

doi:10.1016/j.yexcr.2017.12.025

Cited by 17 publications

(14 citation statements)

References 45 publications

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“…This culture system has been widely used as a model for pro-contractile remodeling of the extracellular matrix by dermal fibroblasts for situations such as wound healing [ 37 ]. This fibroblast-embedded collagen gels system allows cells to develop endogenous tension through both mechanical and biochemical signals relevant for cell contractility, thereby triggering signal transduction cascades capable of modulating transmembrane and/or intracellular receptors to promote intracellular responses such as gene expression and protein synthesis [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

Lupeol, a Pentacyclic Triterpene, Promotes Migration, Wound Closure, and Contractile Effect In Vitro: Possible Involvement of PI3K/Akt and p38/ERK/MAPK Pathways

et al. 2018

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Skin wound healing is a dynamic and complex process involving several mediators at the cellular and molecular levels. Lupeol, a phytoconstituent belonging to the triterpenes class, is found in several fruit plants and medicinal plants that have been the object of study in the treatment of various diseases, including skin wounds. Various medicinal properties of lupeol have been reported in the literature, including anti-inflammatory, antioxidant, anti-diabetic, and anti-mutagenic effects. We investigated the effects of lupeol (0.1, 1, 10, and 20 μg/mL) on in vitro wound healing assays and signaling mechanisms in human neonatal foreskin keratinocytes and fibroblasts. Results showed that, at high concentrations, Lupeol reduced cell proliferation of both keratinocytes and fibroblasts, but increased in vitro wound healing in keratinocytes and promoted the contraction of dermal fibroblasts in the collagen gel matrix. This triterpene positively regulated matrix metalloproteinase (MMP)-2 and inhibited the NF-κB expression in keratinocytes, suggesting an anti-inflammatory effect. Lupeol also modulated the expression of keratin 16 according to the concentration tested. Additionally, in keratinocytes, lupeol treatment resulted in the activation of Akt, p38, and Tie-2, which are signaling proteins involved in cell proliferation and migration, angiogenesis, and tissue repair. These findings suggest that lupeol has therapeutic potential for accelerating wound healing.

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Section: Discussionmentioning

confidence: 99%

Lupeol, a Pentacyclic Triterpene, Promotes Migration, Wound Closure, and Contractile Effect In Vitro: Possible Involvement of PI3K/Akt and p38/ERK/MAPK Pathways

et al. 2018

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“…3) Complete deficiency of tenascin-X (TNX) causes an autosomal recessive classic type of EDS that is characterized by joint hypermobility, hyperelastic skin, and easy bruising without atrophic scarring. 4) TNX, an extracellular protein, plays essential roles in fibrillogenesis, 5) epithelial-to-mesenchymal transition, 6) and wound healing. 7) In 1997, EDS was classified into six types including hEDS.…”

Section: Introductionmentioning

confidence: 99%

“…4) TNX, an extracellular protein, plays essential roles in fibrillogenesis, 5) epithelial-to-mesenchymal transition, 6) and wound healing. 7) In 1997, EDS was classified into six types including hEDS. 8) Initially, Zweers et al reported that haploinsufficiency of TNXB was associated with JHS/hEDS in a small subset of the patients.…”

Section: Introductionmentioning

confidence: 99%

Measurement of Serum Tenascin-X in Joint Hypermobility Syndrome Patients

Yamada

Watanabe

Takeshita

et al. 2019

Biological & Pharmaceutical Bulletin

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Joint hypermobility syndrome (JHS) (also termed hypermobility type Ehlers-Danlos syndrome, hEDS) is a heritable connective tissue disorder that is characterized by generalized joint hypermobility, chronic pain, fatigue, and minor skin changes. Initially, it was reported that there is a small subset of patients with JHS/hEDS who have haploinsufficiency of tenascin-X (TNX). However, the relationship between TNXB and JHS/hEDS has not been reported at all afterwards. EDS was reclassified into thirteen types in 2017, and the causative gene of JHS/hEDS remained to be identified. Therefore, in this study in order to determine whether JHS/hEDS can be diagnosed by the concentrations of serum form of TNX (sTNX), we measured the concentrations of sTNX in 17 JHS/hEDS patients. The sTNX concentrations in half of the JHS/hEDS patients were significantly lower than those in healthy individuals. No mutations, insertions or deletions were detected in the TNX exon sequence of the JHS/hEDS patients except for one in patient. That patient has a heterozygous mutation. A correlation between sTNX concentration and mutation of the TNXB genomic sequence was not found in the JHS/hEDS patients. These results indicate that the decrease in sTNX concentration could be used as a risk factor for JHS/hEDS.

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“…Real‐time RT‐PCR analysis was performed to quantify Vn , αv integrin , Srebp , Acc , Fas , Cd11b , F4/80 , Tnf‐α , Il‐1β , α‐Sma and Tgf‐β1 mRNA expression levels. The primer sequences used in the present experiment are listed in Table . The mRNA expression levels were quantified using the KOD SYBR qPCR Mix (Toyobo Co., Ltd.) with an ABI 7300 real‐time PCR machine (Thermo Fisher Scientific, Waltham, MA).…”

Section: Methodsmentioning

confidence: 99%

“…The primer sequences used in the present experiment are listed in Table 1. [14][15][16][17][18][19][20] The mRNA expression levels were quantified using the KOD SYBR qPCR Mix (Toyobo Co., Ltd.) with an ABI 7300 real-time PCR machine (Thermo Fisher Scientific, Waltham, MA). In all experiments, gene expression levels were determined using the ΔΔCT method after normalization to Gapdh expression levels.…”

Section: Real-time Rt-pcrmentioning

confidence: 99%

Vitronectin deficiency attenuates hepatic fibrosis in a non‐alcoholic steatohepatitis‐induced mouse model

Hayashida

Hashimoto

Ishikawa

et al. 2019

Int J Experimental Path

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Summary Vitronectin (VN), an extracellular matrix protein, is a promising immune biomarker of non‐alcoholic steatohepatitis (NASH); however, its precise function remains unclear. This study investigated how VN deficiency contributes to the development of NASH. Towards this aim, wild‐type (WT) and VN−/− mice were fed with a choline‐deficient, L‐amino acid‐defined, high‐fat diet (CDAHFD) for 6 and 10 weeks to induce NASH, and the livers were isolated. In WT mice fed with CDAHFD for 6 and 10 weeks, the expression of Vn mRNA and protein was up‐regulated compared with that in mice fed with the MF control diet, indicating that VN is regulated in NASH condition. VN−/− mice showed decreased picrosirius red staining in the liver area and Col1a2 mRNA expression levels, compared with WT mice, indicating that the severity of hepatic fibrosis is attenuated in the CDAHFD‐fed VN−/− mice. In addition, VN deficiency did not affect the area of lipid droplets in haematoxylin‐eosin staining and the mRNA expression levels of fatty acid synthases, Srebp, Acc and Fas in the CDAHFD‐fed mice. Moreover, VN deficiency decreased the inflammation score and the mRNA expression levels of Cd11b and F4/80, macrophage markers, as well as Tnf‐α and Il‐1β, inflammatory cytokines in the CDAHFD‐fed mice. Furthermore, VN deficiency decreased the protein and mRNA expression levels of α‐smooth muscle actin in the CDAHFD‐fed mice, suggesting that VN deficiency inhibits the activation of hepatic stellate cells (HSCs). Our findings indicate that VN contributes to the development of fibrosis in the NASH model mice via modulation of the inflammatory reaction and activation of HSCs.

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Wound healing-related properties detected in an experimental model with a collagen gel contraction assay are affected in the absence of tenascin-X

Cited by 17 publications

References 45 publications

Lupeol, a Pentacyclic Triterpene, Promotes Migration, Wound Closure, and Contractile Effect In Vitro: Possible Involvement of PI3K/Akt and p38/ERK/MAPK Pathways

Lupeol, a Pentacyclic Triterpene, Promotes Migration, Wound Closure, and Contractile Effect In Vitro: Possible Involvement of PI3K/Akt and p38/ERK/MAPK Pathways

Measurement of Serum Tenascin-X in Joint Hypermobility Syndrome Patients

Vitronectin deficiency attenuates hepatic fibrosis in a non‐alcoholic steatohepatitis‐induced mouse model

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