miR-106b-responsive gene landscape identifies regulation of Kruppel-like factor family

Wehrkamp, Cody J.; Natarajan, Sathish Kumar; Mohr, Ashley M.; Phillippi, Mary Anne; Mott, Justin L.

doi:10.1080/15476286.2017.1422471

Cited by 9 publications

(12 citation statements)

References 51 publications

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“…Exosomal miR-25-3p promoted colorectal cancer vascular permeability and angiogenesis by targeting KLF2 [ 35 ]. miR-106b possesses an important role in cholangiocarcinoma tumor biology by repressing KLF2 [ 36 ]. In total, ATOH8 and KLF2 may be related to LASC, which needs further study.…”

Section: Discussionmentioning

confidence: 99%

Bioinformatics Analysis of mRNAs and miRNAs for Identifying Potential Biomarkers in Lung Adenosquamous Carcinoma

Nie

Gong

Zhu

et al. 2022

Computational and Mathematical Methods in Medicine

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Background. Lung adenosquamous carcinoma (LASC) is a special type of lung cancer. LASC is a malignant tumor with strong aggressiveness and a poor prognosis. Previous studies have revealed that microRNAs (miRNAs) are widely involved in the development of tumors by targeting mRNA. This study is aimed at identifying the key mRNAs and miRNAs of LASC and constructing miRNA-mRNA networks for deeply comprehending the latent molecular mechanisms. Methods. mRNA dataset (GSE51852) and miRNA dataset (GSE51853) were extracted and downloaded from the Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) and differentially expressed miRNAs (DEMs) were picked out by the GEO2R web tool. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional enrichment analyses were conducted in the DAVID database. The protein-protein interaction (PPI) network was performed and analyzed by using the STRING database and Cytoscape software, respectively. TransmiR v2.0 was applied to predict potential transcription factors of miRNAs. The target genes of DEMs were predicted in the miRWalk database. Results. In comparison to normal tissues, a total of 1458 DEGs (511 upregulated and 947 downregulated) and 13 DEMs (5 upregulated and 8 downregulated) were screened out in LASC tissues. The PPI network of the DEGs displayed five key modules and seventeen hub genes. Six target genes of the DEMs were predicted, and five essential miRNA-mRNA regulatory pairs were established. Ensuingly, CENPF, one of the target genes, was also the hub genes of GSE51852, which was obtained from MCODE and cytoHubba and regulated by hsa-miR-205. Conclusions. We constructed the miRNA-mRNA regulatory pairs, which are helpful to study the potential regulatory mechanisms and find out promising diagnosis biomarkers and therapeutic targets for LASC.

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Section: Discussionmentioning

confidence: 99%

Bioinformatics Analysis of mRNAs and miRNAs for Identifying Potential Biomarkers in Lung Adenosquamous Carcinoma

Nie

Gong

Zhu

et al. 2022

Computational and Mathematical Methods in Medicine

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“…However, the cbt 3′UTR is predicted to contain binding sites for other miRNAs including miR-2 family miRNAs as well as miR-283, raising the possibility that an analogous mechanism may control adaptive responses of other epithelial tissues during development. In addition, vertebrate Cbt orthologs KLF10 and KLF 11 are expressed in numerous epithelial tissues and are regulated by multiple miRNAs including miR-106b and miR-570, suggesting that this mechanism of adaptive response may be evolutionarily conserved (36,45,46).…”

Section: Discussionmentioning

confidence: 99%

A stress-responsive miRNA regulates BMP signaling to maintain tissue homeostasis

Mukherjee

Paricio

Sokol

2021

Proc. Natl. Acad. Sci. U.S.A.

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Adult organisms must sense and adapt to environmental fluctuations. In high-turnover tissues such as the intestine, these adaptive responses require rapid changes in gene expression that, in turn, likely involve posttranscriptional gene control. However, intestinal-tissue–specific microRNA (miRNA)-mediated regulatory pathways remain unexplored. Here, we report the role of an intestinal-specific miRNA, miR-958, that non–cell autonomously regulates stem cell numbers during tissue homeostasis and regeneration in the Drosophila adult midgut. We identify its downstream target cabut, the Drosophila ortholog of mammalian KLF10/11 transcription factors, which mediates this miR-958 function by promoting paracrine enterocyte-to-stem-cell bone morphogenetic protein (BMP) signaling. We also show that mature miR-958 levels transiently decrease in response to stress and that this decrease is required for proper stem cell expansion during tissue regeneration. In summary, we have identified a posttranscriptional mechanism that modulates BMP signaling activity within Drosophila adult intestinal tissue during both normal homeostasis and tissue regeneration to regulate intestinal stem cell numbers.

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“…KLF10 enhances human leukemia cell death by upregulating Bim and Bax pro-apoptotic proteins ( Jin et al, 2007 ). Within cholangiocarcinoma, KLF10 could be regulated by miR-106b and might participate in the anti-apoptotic effects of miR-106b on cholangiocarcinoma cells ( Wehrkamp et al, 2018 ). Thus, KLF10 might be involved in the inhibition of PDT on gemcitabine-resistant cholangiocarcinoma cells.…”

Section: Discussionmentioning

confidence: 99%

Effect of Photodynamic Therapy on Gemcitabine-Resistant Cholangiocarcinoma in vitro and in vivo Through KLF10 and EGFR

Yang

Yao

et al. 2021

Front. Cell Dev. Biol.

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Cholangiocarcinoma is a relatively rare neoplasm with increasing incidence. Although chemotherapeutic agent such as gemcitabine has long been used as standard treatment for cholangiocarcinoma, the interindividual variability in target and drug sensitivity and specificity may lead to therapeutic resistance. In the present study, we found that photodynamic therapy (PDT) treatment inhibited gemcitabine-resistant cholangiocarcinoma cells via repressing cell viability, enhancing cell apoptosis, and eliciting G1 cell cycle arrest through modulating Cyclin D1 and caspase 3 cleavage. In vivo, PDT treatment significantly inhibited the growth of gemcitabine-resistant cholangiocarcinoma cell-derived tumors. Online data mining and experimental analyses indicate that KLF10 expression was induced, whereas EGFR expression was downregulated by PDT treatment; KLF10 targeted the EGFR promoter region to inhibit EGFR transcription. Under PDT treatment, EGFR overexpression and KLF10 silencing attenuated the anti-cancer effects of PDT on gemcitabine-resistant cholangiocarcinoma cells by promoting cell viability, inhibiting apoptosis, and increasing S phase cell proportion. Importantly, under PDT treatment, the effects of KLF10 silencing were significantly reversed by EGFR silencing. In conclusion, PDT treatment induces KLF10 expression and downregulates EGFR expression. KLF10 binds to EGFR promoter region to inhibit EGFR transcription. The KLF10/EGFR axis participates in the process of the inhibition of PDT on gemcitabine-resistant cholangiocarcinoma cells.

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miR-106b-responsive gene landscape identifies regulation of Kruppel-like factor family

Cited by 9 publications

References 51 publications

Bioinformatics Analysis of mRNAs and miRNAs for Identifying Potential Biomarkers in Lung Adenosquamous Carcinoma

Bioinformatics Analysis of mRNAs and miRNAs for Identifying Potential Biomarkers in Lung Adenosquamous Carcinoma

A stress-responsive miRNA regulates BMP signaling to maintain tissue homeostasis

Effect of Photodynamic Therapy on Gemcitabine-Resistant Cholangiocarcinoma in vitro and in vivo Through KLF10 and EGFR

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