Ulvan-chitosan polyelectrolyte complexes as matrices for enzyme induced biomimetic mineralization

Abstract

Polyelectrolyte complexes (PEC) of chitosan and ulvan were fabricated to study ALP mediated formation of apatitic minerals. Scaffolds of the PEC were subjected to alkaline phosphatase (ALP) and successful mineral formation was studied using SEM, Raman and XRD techniques. Investigation of the morphology via SEM shows globular structures of the deposited minerals, which promoted cell attachment, proliferation and extracellular matrix formation. The PEC and their successful calcium phosphate based mineralization offers a greener route of scaffold fabrication towards developing resorbable materials for tissue engineering.

Hybrid Sponge-Like Scaffolds Based on Ulvan and Gelatin: Design, Characterization and Evaluation of Their Potential Use in Bone Tissue Engineering

“…Nevertheless, the potential of ulvan as a biomedical polymer has not yet been exhaustively investigated. To date, a number of ulvan-based scaffolds [32][33][34][35][36][37], as well as polyelectrolyte complex materials [31,38,39], 2D crosslinked membranes [40] and polymer blended nanofibers [41,42] have been prepared.…”

“…Over the past years, efforts have focused on the development of biopolymer-based 3D scaffolds for a wide spectrum of biomedical applications. Both ulvan and gelatin have exhibited osteoinductive potential, either alone or in combination with other materials so as to prepare scaffolds for osseous tissue regeneration [31,34,36,38,43,56,[61][62][63].…”

Biocompatible non-covalent complexes of chitosan and different polymers: Characteristics and application in drug delivery

“…There are several other natural polysaccharides (and their semi-synthetic derivatives) that can form PECs with chitosan. Although they have not been extensively investigated as drug carriers so far, many of them have a certain potential: for colon-specific drug delivery (44,45); gum kondagogu for improved oral delivery of diclofenac-sodium (46); carboxymethylated psyllium arabinoxylan for prolonged release of ibuprofen (47); ulvan for the preparation of matrices for enzyme-induced biomimetic bone mineralization (48); carboxymethyl gum katira for the prolonged of ofloxacin release (49); curdlan sulphate for controlled and pH-dependent zidovudine release (50); carboxylic curdlan for sustained 5-fluorouracil delivery (51); carboxymethyl starch for extended paracetamol release (52).…”

Designing of Chitosan Derivatives Nanoparticles with Antiangiogenic Effect for Cancer Therapy

“…In this context, the use of antioxidants in the form of nanoparticles could improve the efficiency of this therapy due to specific surface area of nanostructures [4], thereby ensuring better contact with cells that would increase the chances of pathological angiogenesis inhibition. However, to have more success in cancer therapy, more studies should be conducted in this direction.Chitosan is a natural cationic polymer exhibits three chemical reactive sites including a primary amine and two primary or secondary hydroxyl groups for further modification [5][6][7]. Nano/microparticles coated with chitosan, synthesized through different techniques for encapsulation (chemical, physical and mechanical techniques), are in continuous development, aiming at the entrapment of bioactive substances, enzymes, hormones, antimicrobial agents, vitamins, minerals, antioxidant agents, and nutrients [8,9].…”

“…Chitosan is a natural cationic polymer exhibits three chemical reactive sites including a primary amine and two primary or secondary hydroxyl groups for further modification [5][6][7]. Nano/microparticles coated with chitosan, synthesized through different techniques for encapsulation (chemical, physical and mechanical techniques), are in continuous development, aiming at the entrapment of bioactive substances, enzymes, hormones, antimicrobial agents, vitamins, minerals, antioxidant agents, and nutrients [8,9].…”