Identification of the AQP members involved in abiotic stress responses from Arabidopsis

Zheng, Feng; Xu, Shengchun; Liu, Na; Zhang, Gu-Wen; Hu, Qizan; Xu, Zhao-Shi; Gong, Yaming

doi:10.1016/j.gene.2017.12.048

Cited by 42 publications

(33 citation statements)

References 52 publications

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“…Some transcripts of GmAQPs were expressed at high levels while others were expressed at low levels (Figure 3). Most GmAQPs extensively functioned in multiple tissues, and individual GmAQPs seemed to function in specific tissue, which was highly similar to AQPs in other plant species [11,12,13,14,15,16]. Some AQP gene pairs at the same evolutionary clade preferred to express in the same tissue.…”

Section: Discussionmentioning

confidence: 78%

“…Among them, eight GmAQPs ( GmPIP1;7 , GmPIP1;8 , GmPIP2;4 , GmPIP2;5 , GmPIP2;13 , GmPIP2;14 , GmTIP1;7 , and GmTIP2;2 ) were favorably accumulated in roots under heat stress. Based on the public database of Arabidopsis eFP Browser [16,43], systematical microarray analyses also showed that most AtAQPs were involved in the heat stress process (Figure S8; Table S1). All these results indicate that plant AQPs serve as targets for modulating thermo-tolerance.…”

Section: Discussionmentioning

confidence: 99%

“…AQPs extensively participated in plant physiological processes under variable environmental stresses [9,10]. Transcript profiles or gene function analyses of AQPs from many plant species, such as Arabidopsis , rice, barley, sorghum, cassava, soybean, and potato, demonstrated that they were associated with drought, cold, salt, silicon, or ABA stress [11,12,13,14,15,16,17]. Recently, several publications reported that AQPs were involved in response to heat stress.…”

Section: Introductionmentioning

confidence: 99%

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Investigation of the AQP Family in Soybean and the Promoter Activity of TIP2;6 in Heat Stress and Hormone Responses

Zheng

Liu

Zhang

et al. 2019

IJMS

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Aquaporins (AQPs) are one diverse family of membrane channel proteins that play crucial regulatory roles in plant stress physiology. However, the heat stress responsiveness of AQP genes in soybean remains poorly understood. In this study, 75 non-redundant AQP encoding genes were identified in soybean. Multiple sequence alignments showed that all GmAQP proteins possessed the conserved regions, which contained 6 trans-membrane domains (TM1 to TM6). Different GmAQP members consisted of distinct Asn-Pro-Ala (NPA) motifs, aromatic/arginine (ar/R) selectivity filters and Froger’s positions (FPs). Phylogenetic analyses distinguished five sub-families within these GmAQPs: 24 GmPIPs, 24 GmTIPs, 17 GmNIPs, 8 GmSIPs, and 2 GmXIPs. Promoter cis-acting elements analyses revealed that distinct number and composition of heat stress and hormone responsive elements existed in different promoter regions of GmAQPs. QRT-PCR assays demonstrated that 12 candidate GmAQPs with relatively extensive expression in various tissues or high expression levels in root or leaf exhibited different expression changes under heat stress and hormone cues (abscisic acid (ABA), l-aminocyclopropane-l-carboxylic acid (ACC), salicylic acid (SA) and methyl jasmonate (MeJA)). Furthermore, the promoter activity of one previously functionally unknown AQP gene-GmTIP2;6 was investigated in transgenic Arabidopsis plants. The beta-glucuronidase (GUS) activity driven by the promoter of GmTIP2;6 was strongly induced in the heat- and ACC-treated transgenic plants and tended to be accumulated in the hypocotyls, vascular bundles, and leaf trichomes. These results will contribute to uncovering the potential functions and molecular mechanisms of soybean GmAQPs in mediating heat stress and hormone signal responses.

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Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Investigation of the AQP Family in Soybean and the Promoter Activity of TIP2;6 in Heat Stress and Hormone Responses

Zheng

Liu

Zhang

et al. 2019

IJMS

Self Cite

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“…In Nicotiana glauca, the NgMIP2 and NgMIP3 genes, which are homologous to TIP, were down-regulated in leaves under drought stress when the leaves were wilting, usually three to four days after water was withheld [86] and in Arabidopsis, the levels of AtTIP1;1, AtTIP1;2, AtTIP2;1 and AtTIP2;2 were more than four-fold down-regulated after 12 days of drought [87]. In contrast to these results, the expression level of AtTIP1;1 and AtTIP2;1 under drought treatment was up-regulated, but the time that the stress was applied was only 24 hours [88]. Moreover, in barley (Hordeum vulgare L.), the expression of five HvTIP aquaporins in leaves: HvTIP1;1, HvTIP1;2, HvTIP2;1, HvTIP2;2 and HvTIP2;3 was down-regulated after ten days of severe drought treatment [89].…”

Section: Drought Salinity and Cold Stressmentioning

confidence: 79%

TIP Aquaporins in Plants: Role in Abiotic Stress Tolerance

Kurowska¹

2021

Abiotic Stress in Plants

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Tonoplast Intrinsic Proteins (TIP) are one of five subfamilies of aquaporins in higher plants. Plants typically contain a large number of TIP genes, ranging from 6 to 35 compared to humans. The molecular weight of the TIP subfamily members ranges from 25 to 28 kDa. Despite their sequence diversity, all TIP monomers have the same structure, which consists of six transmembrane helices and five inter-helical loops that form an hourglass shape with a central pore. Four monomers form tetramers, which are functional units in the membrane. TIPs form channels in the tonoplast that basically function as regulators of the intracellular water flow, which implies that they have a role in regulating cell turgor. TIPs are responsible for precisely regulating the movement of not only water, but also some small neutral molecules such as glycerol, urea, ammonia, hydrogen peroxide and formamide. The expression of TIPs may be affected by different environmental stresses, including drought, salinity and cold. TIPs expression is also altered by phytohormones and the appropriate cis-regulatory motifs are identified in the promotor region of the genes encoding TIPs in different plant species. It was shown that manipulating TIP-encoding genes expression in plants could have the potential to improve abiotic stress tolerance.

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“…2). Moreover, the gene structures of EsNIP4;1, EsNIP4;2, AtNIP4;1 and AtNIP4;2 were identical and had 5 exons, and the length of each exon (132,225,198,62, and 235 bp) was consistent (Tabata et al, 2000;Feng et al, 2017). Exon-intron structural divergences happened commonly in duplicate gene evolution and even in sibling paralogs; these changes occurred through the mechanisms of gain/loss, exonization/pseudoexonization and insertion/deletion (Xu et al, 2012).…”

Section: Discussionmentioning

confidence: 95%

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Supplemental Information 3: Specificity Determining Positions (SDPs) Analysis of E.salsugineum AQPs From Alignments With

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Aquaporins (AQPs) serve as water channel proteins and belong to major intrinsic proteins (MIPs) family, functioned in rapidly and selectively transporting water and other small solutes across biological membranes. Importantly, AQPs have been shown to play critical roles in abiotic stress response of plants. Eutrema salsugineum is close to Arabidopsis thaliana and proposed as a model system for studying plant salt resistance. Here we identified 35 full-length AQP genes in E. salsugineum. Phylogenetic analysis showed EsAQPs were similar with AtAQPs and grouped into four subfamilies including 12 plasma membrane intrinsic proteins (PIPs), 11 tonoplast intrinsic proteins (TIPs), 9 NOD-like intrinsic proteins (NIPs), and 3 small basic intrinsic proteins (SIPs). Gene structure, also the conserved motifs (MEME) of EsAQPs in each subfamily shared high similarities. In detailed sequence analysis, EsAQPs comprised 237-323 amino acids, with a theoretical molecular weight (MW) of 24.31-31.80 kDa and an isoelectric point (pI) value of 4.73-10.49. Functional prediction based on the NPA motif, aromatic/arginine (ar/R) selectivity filter, Froger's position and specificity-determining position suggested there was a big difference in the specificity of substrate transport between EsAQPs. Gene expression profiles illustrated EsAQP genes could be detected in all organs and appear to play an important role in response salt, cold and drought signals. These results will bring a better understanding on the characterizations of AQPs in E. salsugineum and its complex transport networks in homeostasis control.

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Identification of the AQP members involved in abiotic stress responses from Arabidopsis

Cited by 42 publications

References 52 publications

Investigation of the AQP Family in Soybean and the Promoter Activity of TIP2;6 in Heat Stress and Hormone Responses

Investigation of the AQP Family in Soybean and the Promoter Activity of TIP2;6 in Heat Stress and Hormone Responses

TIP Aquaporins in Plants: Role in Abiotic Stress Tolerance

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