Generation of three-dimensional retinal organoids expressing rhodopsin and S- and M-cone opsins from mouse stem cells

Ueda, Kaori; Onishi, Akishi; Ito, Shinichiro; Nakamura, Makoto; Takahashi, Masayo

doi:10.1016/j.bbrc.2017.12.092

Cited by 18 publications

(19 citation statements)

References 34 publications

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“…2,4,29 Instead, retinal progenitor cells and photoreceptor precursor cells derived from pluripotent stem cells (either embryonic stem cells or iPSCs) are increasingly used for retina tissue modeling and cell replacement therapy. [2][3][4][5][6][7][8][9]29 Thus, maintenance of neural retinal fate is a critical characteristic of scaffolds used for retinal tissueengineering materials, since these mitotic cell types can in some cases unintentionally revert to a more potent state or fail to differentiate into mature retinal phenotypes if not provided the correct mechanical or biochemical ques.…”

Section: Discussionmentioning

confidence: 99%

“…R ecent advances in genome editing 1,2 and threedimensional (3D) retinal differentiation (ie, organoids or spheroids) [3][4][5][6][7][8][9] have taken in vitro retinal disease models closer to the in vivo state than ever before by enabling the study of genotype-to-phenotype relationship, and by introducing inherent intercellular interactions, respectively. However, induced pluripotent stem cell (iPSC)-derived retinal spheroids are often heterogeneous and their development can be inconsistent.…”

Section: Introductionmentioning

confidence: 99%

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Development of High-Resolution Three-Dimensional-Printed Extracellular Matrix Scaffolds and Their Compatibility with Pluripotent Stem Cells and Early Retinal Cells

Shrestha

Allen

Wiley

et al. 2020

Journal of Ocular Pharmacology and Therapeutics

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Purpose: Widely used approaches for retinal disease modeling and in vitro therapeutic testing can be augmented by using tissue-engineered scaffolds with a precise 3-dimensional structure. However, the materials currently used for these scaffolds are poorly matched to the biochemical and mechanical properties of the in vivo retina. Here, we create biopolymer-based scaffolds with a structure that is amenable to retinal tissue engineering and modeling. Methods: Optimal two-photon polymerization (TPP) settings, including laser power and scanning speed, are identified for 4 methacrylated biopolymer formulations: collagen, gelatin, hyaluronic acid (HA), and a 50/50 mixture of gelatin/HA, each with methylene blue as a photoinitiator. For select formulations, fabrication accuracy and swelling are determined and biocompatibility is evaluated by using human induced pluripotent stem cells and rat postnatal retinal cells. Results: TPP is feasible for each biopolymer formulation, but it is the most reliable for mixtures containing gelatin and the least reliable for HA alone. The mean size of microscaffold pores is within several microns of the intended value but the overall structure size is several times greater than the modeled volume. The addition of HA to gelatin scaffolds increases cell viability and promotes neuronal phenotype, including Tuj-1 expression and characteristic morphology. Conclusion: We successfully determined a useful range of TPP settings for 4 methacrylated biopolymer formulations. When crosslinked, these extracellular matrix-derived molecules support the growth and attachment of retinal cells. We anticipate that when combined with existing patient-specific approaches, this technique will enable more efficient and accurate retinal disease modeling and therapeutic testing in vitro than current techniques allow.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of High-Resolution Three-Dimensional-Printed Extracellular Matrix Scaffolds and Their Compatibility with Pluripotent Stem Cells and Early Retinal Cells

Shrestha

Allen

Wiley

et al. 2020

Journal of Ocular Pharmacology and Therapeutics

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“…3D printing is as ground-breaking fabrication process o®ering innovative solutions in variety of¯elds including tissue engineering, 172,173 cancer therapy 174 and generation of arti¯cial organ. 175 3D printing combined with advanced techniques of material design and fabrication is even capable to fabricate materials for arti¯cial sca®olds for bone reconstruction and regeneration. 176 Phosphorus is most important elements for human bones.…”

Section: Bp For 3d Printing Sca®oldsmentioning

confidence: 99%

Black phosphorus as a versatile nanoplatform: From unique properties to biomedical applications

Xiong

Chen

Liu

et al. 2020

J. Innov. Opt. Health Sci.

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Black phosphorus (BP) or phosphorene, a new superstar among two-dimensional (2D) materials, has sparked huge scientific interest since its discovery in 2014. BP offers unique characteristics including high drug loading efficiency, excellent photodynamic and photothermal properties and good biocompatibility. These characteristics expand versatility of BP in nanomedicine. Although the outlook of BP seems promising, its practical biomedical applications are still at the very initial stage especially in comparison to other thoroughly investigated inorganic nanomaterials. This paper reviews BP structure and properties as well as its preparation approaches with the emphasis on techniques to improve BP stability and biocompatibility for their further usage in physiological environment. Meanwhile, recent progress made in various biomedical research fields from bioimaging to biosensing is discussed. Last, but not least, current challenges and prospects for BP in biomedicine are briefly examined, which will be useful to guide future developments of BP.

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“…The first organoid was created 9 years ago from a single intestinal crypt cell; where even in the absence of a non-epithelial cellular niche, a single cell was able to self-renew and differentiate, generating a villus-like epithelial structure in which all the different cell types present in the real organ were found (Sato et al, 2009). Since then several "mini-tissue" have been reproduced successfully in many animals including human pancreas, kidney, thyroid, liver, inner ear, retina, skin tissue with follicular hair and brain (Barkauskas et al, 2017;Lee et al, 2018;Koehler et al, 2017;Orsini et al, 2018;Ramachandran et al, 2015;Turksen, 2016;Ueda et al, 2018).…”

Section: Organoids As Models For the Study Of Human Nasal Diseasesmentioning

confidence: 99%

Comparative models for human nasal infections and immunity

Casadei

Salinas

2019

Developmental & Comparative Immunology

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Generation of three-dimensional retinal organoids expressing rhodopsin and S- and M-cone opsins from mouse stem cells

Cited by 18 publications

References 34 publications

Development of High-Resolution Three-Dimensional-Printed Extracellular Matrix Scaffolds and Their Compatibility with Pluripotent Stem Cells and Early Retinal Cells

Development of High-Resolution Three-Dimensional-Printed Extracellular Matrix Scaffolds and Their Compatibility with Pluripotent Stem Cells and Early Retinal Cells

Black phosphorus as a versatile nanoplatform: From unique properties to biomedical applications

Comparative models for human nasal infections and immunity

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