Lipid-mediated regulation of pore-forming activity of syringomycin E by thyroid hormones and xanthene dyes

Efimova, Svetlana S.; Zakharova, Anastasiia A.; Ismagilov, Artem A.; Schagina, Ludmila V.; Malev, V. V.; Bashkirov, Pavel V.; Ostroumova, Olga S.

doi:10.1016/j.bbamem.2017.12.010

Cited by 11 publications

(11 citation statements)

References 51 publications

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“…3b. The mean number of open pores under the steady-state conditions, N ch ( V ), is concerned to the excess channel formation work, W ch = W ch ( V ) − W ch (0), as determined by the Boltzmann distribution 30 :where N ch (0), the number of channels at V = 0, is a constant owing to equilibrium between the precursors of the channels in the membrane and FE molecules in the bathing solution, k is a Boltzmann constant, and T is an absolute temperature.…”

Section: Resultsmentioning

confidence: 99%

Fengycin induces ion channels in lipid bilayers mimicking target fungal cell membranes

Zakharova

Efimova

Malev

et al. 2019

Sci Rep

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The one-sided addition of fengycin (FE) to planar lipid bilayers mimicking target fungal cell membranes up to 0.1 to 0.5 μM in the membrane bathing solution leads to the formation of well-defined and well-reproducible single-ion channels of various conductances in the picosiemens range. FE channels were characterized by asymmetric conductance-voltage characteristic. Membranes treated with FE showed nonideal cationic selectivity in potassium chloride bathing solutions. The membrane conductance induced by FE increased with the second power of the lipopeptide aqueous concentration, suggesting that at least FE dimers are involved in the formation of conductive subunits. The pore formation ability of FE was not distinctly affected by the molecular shape of membrane lipids but strongly depended on the presence of negatively charged species in the bilayer. FE channels were characterized by weakly pronounced voltage gating. Small molecules known to modify the transmembrane distribution of electrical potential and the lateral pressure profile were used to modulate the channel-forming activity of FE. The observed effects of membrane modifiers were attributed to changes in lipid packing and lipopeptide oligomerization in the membrane.

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Section: Resultsmentioning

confidence: 99%

Fengycin induces ion channels in lipid bilayers mimicking target fungal cell membranes

Zakharova

Efimova

Malev

et al. 2019

Sci Rep

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“…It was shown that SyrE forms asymmetric peptide-lipid pores of a conical shape with predominant anion selectivity ( Malev et al, 2002 ; Ostroumova et al, 2007a ). The properties of single SyrE pores and lipopeptide induced steady-state transmembrane current depend on the boundary potential of membrane ( Feigin et al, 1996 ; Schagina et al, 1998 ; Dalla Serra et al, 1999 ; Ostroumova et al, 2005 , 2007b , 2008 ; Efimova et al, 2018b ). A previous study showed that a decrease in the membrane dipole potential upon addition of small molecule modifiers leads to a reduction of SyrE-pore conductance and dwell time and an increase in the SyrE-induced steady-state transmembrane current ( Ostroumova et al, 2007b , 2008 ; Efimova et al, 2018b ).…”

Section: Resultsmentioning

confidence: 99%

“…The properties of single SyrE pores and lipopeptide induced steady-state transmembrane current depend on the boundary potential of membrane ( Feigin et al, 1996 ; Schagina et al, 1998 ; Dalla Serra et al, 1999 ; Ostroumova et al, 2005 , 2007b , 2008 ; Efimova et al, 2018b ). A previous study showed that a decrease in the membrane dipole potential upon addition of small molecule modifiers leads to a reduction of SyrE-pore conductance and dwell time and an increase in the SyrE-induced steady-state transmembrane current ( Ostroumova et al, 2007b , 2008 ; Efimova et al, 2018b ). The effect on multichannel activity is due to the facilitation of the immersion of positively charged SyrE molecules into the lipid bilayer with a decrease in its dipole potential ( Ostroumova et al, 2008 ; Efimova et al, 2018b ).…”

Section: Resultsmentioning

confidence: 99%

“…A previous study showed that a decrease in the membrane dipole potential upon addition of small molecule modifiers leads to a reduction of SyrE-pore conductance and dwell time and an increase in the SyrE-induced steady-state transmembrane current ( Ostroumova et al, 2007b , 2008 ; Efimova et al, 2018b ). The effect on multichannel activity is due to the facilitation of the immersion of positively charged SyrE molecules into the lipid bilayer with a decrease in its dipole potential ( Ostroumova et al, 2008 ; Efimova et al, 2018b ).…”

Section: Resultsmentioning

confidence: 99%

“…The latter suggestion might be of fundamental importance in terms of the possible synergism of the action of C6 and the antifungal polyene macrolide antibiotics amphotericin and Nys. The pores formed by the one-sided introduction of these antibiotics into the membrane have a lipid mouth with a positive curvature in the opposite monolayer leaflet and low molecular weight membrane modifiers that provoke positive curvature stress might enhance the pore-forming ability of amphotericin and Nys ( Kleinberg and Fninkelstein, 1984 ; Chulkov et al, 2015 ; Efimova et al, 2018a , b ). To test this possibility, we have performed measurements of the steady-state Nys-induced transmembrane current before and after addition of C6.…”

Section: Resultsmentioning

confidence: 99%

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1,3-Thiazine, 1,2,3,4-Dithiadiazole, and Thiohydrazide Derivatives Affect Lipid Bilayer Properties and Ion-Permeable Pores Induced by Antifungals

Zakharova

Efimova

Yuskovets

et al. 2020

Front. Cell Dev. Biol.

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Over the past decade, thiazines, thiadiazoles, and thiohydrazides have attracted increasing attention due to their sedative, antimicrobial, antiviral, antifungal, and antitumor activities. The clinical efficacy of such drugs, as well as the possibility of developing resistance to antimicrobials, will depend on addressing a number of fundamental problems, including the role of membrane lipids during their interaction with plasma membranes. The effects of the eight 1,3-thiazine-, 1,2,3,4-dithiadiazole-, and thiohydrazide-related compounds on the physical properties of model lipid membranes and the effects on reconstituted ion channels induced by the polyene macrolide antimycotic nystatin and antifungal cyclic lipopeptides syringomycin E and fengycin were observed. We found that among the tested agents, the fluorine-containing compound N-(3,5-difluorophenyl)-benzenecarbothiohydrazide (C6) was the most effective at increasing the electric barrier for anion permeation into the hydrophobic region of the membrane and reducing the conductance of anion-permeable syringomycin pores. A decrease in the membrane boundary potential with C6 adsorption also facilitated the immersion of positively charged syringomycin molecules into the lipid bilayer and increases the pore-forming ability of the lipopeptide. Using differential scanning microcalorimetry, we showed that C6 led to disordering of membrane lipids, possibly by potentiating positive curvature stress. Therefore, we used C6 as an agonist of antifungals forming the pores that are sensitive to membrane curvature stress and lipid packing, i.e., nystatin and fengycin. The dramatic increase in transmembrane current induced by syringomycin E, nystatin, and fengycin upon C6 treatment suggests its potential in combination therapy for treating invasive fungal infections.

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AFM‐TEM correlation microscopy and its application to lipid nanoparticles

Bagrov

Adlerberg

Skryabin³

et al. 2023

Microscopy Res & Technique

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So far, only a few articles have demonstrated the possibility of correlated AFM‐TEM imaging – sequential imaging of the same individual objects using atomic‐force microscopy (AFM) and transmission electron microscopy (TEM). The current work contributes to the development of this approach by giving a step‐by‐step procedure, which yields pairs of correlated AFM‐TEM images. We describe the application of correlation AFM‐TEM microscopy to lipid nanoparticles (small extracellular vesicles and liposomes). The sizes of individual particles measured by the two methods were in good agreement, taking the tip broadening into account. The correlated AFM‐TEM imaging can be valuable for single‐particle analysis and nanometrology.

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Lipid-mediated regulation of pore-forming activity of syringomycin E by thyroid hormones and xanthene dyes

Cited by 11 publications

References 51 publications

Fengycin induces ion channels in lipid bilayers mimicking target fungal cell membranes

Fengycin induces ion channels in lipid bilayers mimicking target fungal cell membranes

1,3-Thiazine, 1,2,3,4-Dithiadiazole, and Thiohydrazide Derivatives Affect Lipid Bilayer Properties and Ion-Permeable Pores Induced by Antifungals

AFM‐TEM correlation microscopy and its application to lipid nanoparticles

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