Tissue specific expression and in-silico characterization of a putative cysteine synthase gene from Lathyrus sativus L.

Chakraborty, Saikat; Mitra, Joy; Samanta, Milan Kumar; Sikdar, Narattam; Bhattacharyya, Jagannath; Manna, Anulina; Pradhan, Subrata; Chakraborty, Anirban; Pati, Biswamoy

doi:10.1016/j.gep.2017.12.001

Cited by 8 publications

(6 citation statements)

References 33 publications

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“…The LsCSase gene was up-regulated in young seedling tissues as well as in young seeds with an increased expression level under zinc-iron stress conditions and polyethylene glycol-induced osmotic stress. Hence, this preliminary investigation implies a correlation between the LsCSase gene and the β-ODAP biosynthesis pathway (Chakraborty et al 2018). Further studies are needed, for example through the development of genetically engineered grass pea lines in terms of the CSase gene to explore its exact activity during the β-ODAP biosynthesis.…”

Section: The Biochemistry Behind Neurolathyrismmentioning

confidence: 95%

“…Nevertheless, the enzymatic step leading from BIA to β-ODAP had been studied previously (Ikegami et al 1999). More recently, a novel cysteine synthase (LsCSase) gene from grass pea has been identified and characterized through bioinformatics approaches (Chakraborty et al 2018). The LsCSase gene was up-regulated in young seedling tissues as well as in young seeds with an increased expression level under zinc-iron stress conditions and polyethylene glycol-induced osmotic stress.…”

Section: The Biochemistry Behind Neurolathyrismmentioning

confidence: 99%

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Grass pea (Lathyrus sativus L.): orphan crop, nutraceutical or just plain food?

Lambein

Travella

Kuo

et al. 2019

Planta

136

127

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Main conclusion Although grass pea is an environmentally successful robust legume with major traits of interest for food and nutrition security, the genetic potential of this orphan crop has long been neglected. Grass pea (Lathyrus sativus L.) is a Neolithic plant that has survived millennia of cultivation and has spread over three continents. It is a robust legume crop that is considered one of the most resilient to climate changes and to be survival food during drought-triggered famines. The hardy penetrating root system allows the cultivation of grass pea in various soil types, including marginal ones. As an efficient nitrogen fixer, it meets its own nitrogen requirements and positively benefits subsequent crops. However, already in ancient India and Greece, overconsumption of the seeds and a crippling neurological disorder, later coined neurolathyrism, had been linked. Overemphasis of their suspected toxic properties has led to disregard the plant's exceptionally positive agronomic properties and dietary advantages. In normal socioeconomic and environmental situations, in which grass pea is part of a balanced diet, neurolathyrism is virtually non-existent. The etiology of neurolathyrism has been oversimplified and the deficiency in methionine in the diet has been overlooked. In view of the global climate change, this very adaptable and nutritious orphan crop deserves more attention. Grass pea can become a wonder crop if the double stigma on its reputation as a toxic plant and as food of the poor can be disregarded. Additionally, recent research has exposed the potential of grass pea as a health-promoting nutraceutical. Development of varieties with an improved balance in essential amino acids and diet may be relevant to enhance the nutritional value without jeopardizing the multiple stress tolerance of this promising crop.

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Section: The Biochemistry Behind Neurolathyrismmentioning

confidence: 95%

Section: The Biochemistry Behind Neurolathyrismmentioning

confidence: 99%

Grass pea (Lathyrus sativus L.): orphan crop, nutraceutical or just plain food?

Lambein

Travella

Kuo

et al. 2019

Planta

136

127

View full text Add to dashboard Cite

show abstract

“…All the reactions were conducted in triplicate. β -tubulin of L. sativus was used as internal control and primers for β -tubulin were as per Chakraborty et al (2018). The gene-specific primers for LsAAE3 were designed in such a way so that they anneal to exons flanking intron sequences to identify any potential genomic DNA contamination.…”

Section: Methodsmentioning

confidence: 99%

“…All the reactions were conducted in triplicate. β tubulin of L. sativus was used as internal control and primers for β -tubulin were as per Chakraborty et al (2018).…”

Section: Total Rna Isolation and Real Time Quantitative Pcr Analysismentioning

confidence: 99%

Identification of oxalyl-CoA synthetase gene (LsAAE3) and its regulatory role in β-ODAP biosynthesis in grasspea (Lathyrus sativusL.)

Kushwah

Shanmugavadivel

Das

et al. 2021

Preprint

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Grasspea is a popular pulse crop due to its hardiness and low cost of production. Presence of anti-nutritive factor ‘β-ODAP’ in its seeds and other plant parts hinder its widespread cultivation and usage. Oxalyl-CoA synthetase is one of the key enzyme of β-ODAP biosynthesis pathway, catalyses the conversion of oxalate to oxalyl-CoA. ACYL ACTIVATING ENZYME 3 (AAE3) gene has been characterised to encode an oxalyl-CoA synthetase enzyme in many plant species. We report here the isolation of full length AAE3 homolog in grasspea with a combination of PCR based strategy and in silico analysis. We first identified AAE3 homolog by PCR using degenerate primers. The partial LsAAE3 sequence showed 88% amino acids sequence identity with the characterised AAE3 gene of M. truncatula. We then predicted the full length AAE3 sequence using the publically available transcriptome datasets of grasspea. Determination of LsAAE3 gene and protein structure and phylogenetic relationship analysis strongly suggested that LsAAE3 is a true homolog of AAE3 gene. Expression profiling of LsAAE3 in grasspea varieties with contrast in β-ODAP content revealed its inverse relationship with the β-ODAP content, LsAAE3 thus negatively regulates the synthesis of β-ODAP. Involvement of AAE3 encoded oxalyl-CoA synthetase in a CoA-dependent pathway of oxalate degradation is well proven in many plant species. We also identified the CoA-dependent pathway of oxalate degradation in grasspea. Based on these observations, we hypothesized that LsAAE3 may regulate β-ODAP content, possibly, by CoA-dependent pathway of oxalate degradation in grasspea. If this hypothesis is substantiated, genetic manipulation of LsAAE3 presents viable option for reducing β-ODAP content in grass pea.

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“…A novel cysteine synthase gene (LsCSase) has been discovered in grasspea. Under zinc-iron stress and polyethylene glycol-induced osmotic stress, this gene was up-regulated in young seedling tissues and seeds, with an elevated expression level ( 76 ). Understanding of the fundamental steps in the regulation and the biosynthesis of β-ODAP are significant in breeding new grasspea cultivars.…”

Section: Grasspea and The Case History Of Lathyrismmentioning

confidence: 99%

Rediscovering the Potential of Multifaceted Orphan Legume Grasspea- a Sustainable Resource With High Nutritional Values

et al. 2022

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The genus Lathyrus consists of more than 184 herbaceous annual and perennial species suitable for multifaceted sustainable food and feed production system in the arid and semi-arid regions of the world. The grasspea is a promising source of protein nutrition. However, its potential is not being utilized fully due to the presence of neurotoxin content (β-N-oxalyl-l-α, β diaminopropionic acid, β-ODAP), a causal agent of non-reversible lower limbs paralysis. The high protein contents in seeds and leaves with ~90% digestibility make it sustainable super food to beat protein malnutrition in future. Therefore, it is desired to breed new grasspea cultivars with low β-ODAP contents. Limited research has been carried out to date about this feature. A draft genome sequence of grasspea has been recently published that is expected to play a vital role in breeding and identifying the genes responsible for biosynthesis pathway of β-ODAP contents in grasspea. Efforts to increase awareness about the importance of genus Lathyrus and detoxify β-ODAP in grasspea are desired and are in progress. Presently, in South Asia, systematic and dedicated efforts to support the farmers in the grasspea growing regions by disseminating low β-ODAP varieties has resulted in a considerable improvement in reducing the incidence of neurolathyrism. It is expected that the situation will improve further by mainstreaming grasspea cultivation by implementing different approaches such as the development and use of low β-ODAP varieties, strengthening government policies and improved detox methods. The present review provides insight into the multifaceted characteristics of sustainable nutritious grasspea in the global and Indian perspective.

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Tissue specific expression and in-silico characterization of a putative cysteine synthase gene from Lathyrus sativus L.

Cited by 8 publications

References 33 publications

Grass pea (Lathyrus sativus L.): orphan crop, nutraceutical or just plain food?

Grass pea (Lathyrus sativus L.): orphan crop, nutraceutical or just plain food?

Identification of oxalyl-CoA synthetase gene (LsAAE3) and its regulatory role in β-ODAP biosynthesis in grasspea (Lathyrus sativusL.)

Rediscovering the Potential of Multifaceted Orphan Legume Grasspea- a Sustainable Resource With High Nutritional Values

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