Curcumin inhibits activation induced by urban particulate material or titanium dioxide nanoparticles in primary human endothelial cells

Abstract

Curcumin has protective effects against toxic agents and shows preventive properties for various diseases. Particulate material with an aerodynamic diameter of ≤10 μm (PM10) and titanium dioxide nanoparticles (TiO2-NPs) induce endothelial dysfunction and activation. We explored whether curcumin is able to attenuate different events related to endothelial activation. This includes adhesion, expression of adhesion molecules and oxidative stress induced by PM10 and TiO2-NPs. Human umbilical vein endothelial cells (HUVEC) were treated with 1, 10 and 100 μM curcumin for 1 h and then exposed to PM10 at 3 μg/cm2 or TiO2-NPs at 10 μg/cm2. Cell adhesion was evaluated by co-culture with U937 human myelomonocytic cells. Adhesion molecules expression was measured by flow cytometry after 3 or 24 h of exposure. Oxidative stress was determined by 2,7-dichlorodihydrofluorescein (H2DCF) oxidation. PM10 and TiO2-NPs induced the adhesion of U937 cells and the expression of E- and P-selectins, intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and platelet-endothelial cell adhesion molecule-1 (PECAM-1). The expression of E- and P-selectins matched the adhesion of monocytes to HUVEC after 3 h. In HUVEC treated with 1 or 10 μM curcumin, the expression of adhesion molecules and monocytes adhesion was significantly diminished. Curcumin also partially reduced the H2DCF oxidation induced by PM10 and TiO2-NPs. Our results suggest an anti-inflammatory and antioxidant role by curcumin attenuating the activation caused on endothelial cells by exposure to particles. Therefore, curcumin could be useful in the treatment of diseases where an inflammatory process and endothelial activation are involved.

Potential roles of Kruppel‐like factors in mediating adverse vascular effects of nanomaterials: A review

“…Montiel‐Dávalos et al reported that TiO 2 NPs induced adhesion molecules and the adhesion of monocytes onto HUVECs. Intracellular ROS was also induced, and inhibition of intracellular ROS by curcumin inhibited TiO 2 NP‐induced EC activation, indicating a role of oxidative stress (Montiel‐Dávalos et al, 2017). Interestingly, NM‐induced EC activation may be correlated with cytotoxicity.…”

The effect of nanoparticles of cobalt–chromium on human aortic endothelial cells in vitro

“…It has been reported that ZnO NPs and TiO 2 NPs can increase the secretion of adhesion molecules in HUVEC (C. M. Chen et al, 2020; Montiel‐Dávalos et al, 2017). Besides, decreased endothelial cells viability could lead to not only the loss of cell integrity but also increased vascular permeability, resulting in increased monocytes migration and adhesion (Bobryshev, 2006; M. T. Zhu et al, 2011).…”

Role of curcumin in ischemia and reperfusion injury

“…Evidences showing that curcumin can decrease the level of TNF-α-induced VCAM-1 in vascular endothelial cells and the level of ICAM-1 in aneurysmal walls ( Huyut et al, 2022 ; Fan et al, 2019 ). Otherwise, ( Montiel-Dávalos et al, 2017 ) showed that air pollutants such as inhalable particles with an aerodynamic diameter of ≤10 μm (PM10) and titanium dioxide nanoparticles (TiO2-NPs) could induce the dysfunctions and abnormal activation of endothelial cells, which led to the over expressions of ICAM-1, VCAM-1, E-selectin and P-selectin, causing the excessive inflammatory cells infiltration and damage in the endothelial cells. And their results manifested that curcumin had an anti-inflammatory role by attenuating the dysfunctions and abnormal activation of endothelial cells by the exposures to PM10 and TiO2-NPs.…”