Abstract:Overuse of antibiotics is a major problem in the treatment of bovine mastitis, and antibiotic treatment is frequently non-curative, thus alternative treatments are necessary. The primary aim of this study was to evaluate the efficacy of a purified phage cocktail for treatment of bovine Staphylococcus aureus mastitis in a well-defined mouse model. Candidate phages were selected based on their in vitro performance and subsequently processed into an optimally composed phage cocktail. The highest scoring phages we… Show more
“…It may therefore be useful to develop cocktails consisting of different bacteriophages or to supplement the phage therapy with endolysins. The expansion of the working spectrum has already been tested and confirmed by two recent studies (Porter et al, 2016;Breyne et al, 2017). The main disadvantage of the classical endolysins remains their Gram-positive spectrum.…”
Section: Critical Comments and Future Pros-pectsmentioning
confidence: 68%
“…A selected phage cocktail for the treatment of S. aureus-associated (Newbould 305) bovine mastitis has recently been tested in vitro and in a mouse model (Breyne et al, 2017). First, different cultures were verified in the presence or absence of IgG and the phage cocktail.…”
Section: In Vitro and In Vivo Evaluation Of Bacteriophages In The Conmentioning
confidence: 99%
“…A third group was injected with the phage cocktail. Breyne et al (2017) reported that S. aureus could not be isolated from the mice which were injected with cefalonium. In contrast, S. aureus was still present in the phagetreated group although the number of colony forming units (CFU) was significantly lower when compared to the negative control group.…”
Section: In Vitro and In Vivo Evaluation Of Bacteriophages In The Conmentioning
The increase in bacterial drug resistance causes major difficulties in the clinical treatment of a growing number of bacterial infections worldwide. Consequently, there is an urgent need to develop novel anti-bacterial agents to control these resistant pathogens and to complement the currently used antibiotics. Mastitis is the most prevalent disease impacting dairy cattle, and therefore one of the costliest diseases in the global dairy industry. The excessive use of curative as well as preventive antibiotics in this sector entails a real risk for the emergence of antimicrobial resistance. Moreover, these traditional antimicrobial agents are often ineffective and lead to residues in the milk, which can affect dairy product consumers. As an alternative therapeutic approach, bacteriophages and phage-encoded endolysins have been proposed and are currently (re)investigated as potential antibacterial agents against mastitis.
“…It may therefore be useful to develop cocktails consisting of different bacteriophages or to supplement the phage therapy with endolysins. The expansion of the working spectrum has already been tested and confirmed by two recent studies (Porter et al, 2016;Breyne et al, 2017). The main disadvantage of the classical endolysins remains their Gram-positive spectrum.…”
Section: Critical Comments and Future Pros-pectsmentioning
confidence: 68%
“…A selected phage cocktail for the treatment of S. aureus-associated (Newbould 305) bovine mastitis has recently been tested in vitro and in a mouse model (Breyne et al, 2017). First, different cultures were verified in the presence or absence of IgG and the phage cocktail.…”
Section: In Vitro and In Vivo Evaluation Of Bacteriophages In The Conmentioning
confidence: 99%
“…A third group was injected with the phage cocktail. Breyne et al (2017) reported that S. aureus could not be isolated from the mice which were injected with cefalonium. In contrast, S. aureus was still present in the phagetreated group although the number of colony forming units (CFU) was significantly lower when compared to the negative control group.…”
Section: In Vitro and In Vivo Evaluation Of Bacteriophages In The Conmentioning
The increase in bacterial drug resistance causes major difficulties in the clinical treatment of a growing number of bacterial infections worldwide. Consequently, there is an urgent need to develop novel anti-bacterial agents to control these resistant pathogens and to complement the currently used antibiotics. Mastitis is the most prevalent disease impacting dairy cattle, and therefore one of the costliest diseases in the global dairy industry. The excessive use of curative as well as preventive antibiotics in this sector entails a real risk for the emergence of antimicrobial resistance. Moreover, these traditional antimicrobial agents are often ineffective and lead to residues in the milk, which can affect dairy product consumers. As an alternative therapeutic approach, bacteriophages and phage-encoded endolysins have been proposed and are currently (re)investigated as potential antibacterial agents against mastitis.
“…saw a reduction in the bacterial load in mammary tissue in comparison with the phage‐free control. An improvement in mammary gland pathology was also observed post‐treatment (Breyne et al ). A similar study by Iwano et al .…”
Section: Veterinary Applications Of Phages In Dairy Productionmentioning
Dairy products are susceptible to bacterial contamination during both primary production and the processing stages. Pasteurisation and high hydrostatic pressure are used to eliminate bacteria from milk; however, some heat‐tolerant pathogens and bacterial spores may survive these processes. Bacteriophages (phages) are viruses that infect and lyse specific bacteria. These or their encoded proteins may be exploited for pathogen biocontrol in milk products and for mastitis treatment during primary production. The host specificity of phages and their proteins may also be exploited for pathogen detection. Various examples of phage‐mediated pathogen biocontrol and detection in the dairy context are discussed in this review.
“…S. aureus , one of the etiological agents for mastitis, which has a propensity to recur chronically, causes a potentially fatal inflammatory response in gland tissues. In an experimental model, lactating mice intramammarily infected with a clinical bovine S. aureus strain showed significant improvement in mammary gland pathology and a 4-log reduction in bacterial load after phage treatment [ 32 ]. However, compared to the antibiotic cefalonium, the phage treatment was far less effective.…”
Section: Bacteriophages In Food Animal Productionmentioning
The ability of agriculture to continually provide food to a growing world population is of crucial importance. Bacterial diseases of plants and animals have continually reduced production since the advent of crop cultivation and animal husbandry practices. Antibiotics have been used extensively to mitigate these losses. The rise of antimicrobial resistant (AMR) bacteria, however, together with consumers’ calls for antibiotic-free products, presents problems that threaten sustainable agriculture. Bacteriophages (phages) are proposed as bacterial population control alternatives to antibiotics. Their unique properties make them highly promising but challenging antimicrobials. The use of phages in agriculture also presents a number of unique challenges. This mini-review summarizes recent development and perspectives of phages used as antimicrobial agents in plant and animal agriculture at the farm level. The main pathogens and their adjoining phage therapies are discussed.
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