Global gene expression in two potato cultivars in response to ‘Candidatus Liberibacter solanacearum’ infection

Abstract

BackgroundTranscriptomic analyses were performed to compare the molecular responses of two potato varieties previously shown to differ in the severity of disease symptoms due to infection by “Candidatus Liberibacter solanacearum” (Lso), the causative agent of Zebra Chip in potato. A factorial design utilizing the two varieties and psyllids either harboring Lso or without bacteria was used to discriminate varietal responses to pathogen infection versus psyllid feeding. Plant response was determined from leaf samples 3 weeks after infection.ResultsIn response to Lso infection, 397 genes were differentially expressed in the variety Atlantic (most susceptible) as compared to 1027 genes in Waneta. Over 80% of the transcriptionally-changed genes were down-regulated in both varieties, including genes involved in photosynthesis or primary and secondary metabolism. Many of the Lso-responsive genes involved in stress responses or hormonal pathways were regulated differently in the two potato varieties.ConclusionsThis study focused on the time point just prior to the onset of symptom development and provides valuable insight into the mechanisms of Liberibacter pathogenicity, especially the widespread suppression of plant gene expression, including genes involved in plant defenses.Electronic supplementary materialThe online version of this article (10.1186/s12864-017-4313-2) contains supplementary material, which is available to authorized users.

Genome-wide analysis of lectin receptor-like kinases family from potato (Solanum tuberosum L.)

“…To investigate the responses of StLecRLKs to biotic stress, expression data were obtained from the published studies, including potato infected with pathogenic bacteria Candidatus Liberibacter solanacearum (Cls) (Levy et al, 2017), Pectobacterium carotovorum subsp. brasiliense (Pcb) (Kwenda et al, 2016) and Ralstonia solanacearum (Rs) (Zuluaga et al, 2015), fungi Phytophthora infestans (Pi) (Yogendra & Kushalappa, 2016), and Fusarium sulphureum (Fs) (He et al, 2018).…”

“…Based on the published transcriptomic datasets, the expressional patterns of StLecRLKs were investigated during potato infected with phytopathogenic bacteria (Levy et al, 2017;Kwenda et al, 2016;Zuluaga et al, 2015) and fungi (Yogendra & Kushalappa, 2016;He et al, 2018). For the bacteria, expression changes of StLecRLKs were extracted after potato inoculated with Cls, Pcb and Rs (Fig.…”

Peer Review #2 of "Genome-wide analysis of lectin receptor-like kinases family from potato (Solanum tuberosum L.) (v0.2)"

“…Based on the published transcriptomic datasets, the expressional patterns of StLecRLKs were investigated during potato infected with phytopathogenic bacteria [42][43][44] and fungi [45,46]. For the bacteria, expression changes of Furthermore, StLecRLKs were detected to be up-regulated under at least one bacterial infection.…”

Peer Review #2 of "Genome-wide analysis of lectin receptor-like kinases family from potato (Solanum tuberosum L.) (v0.1)"

“…To investigate the responses of StLecRLKs to biotic stress, expression data were obtained from the published studies, including potato infected with pathogenic bacteria Candidatus Liberibacter solanacearum (Cls) [42], Pectobacterium carotovorum subsp. brasiliense (Pcb) [43] and Ralstonia solanacearum (Rs) [44], fungi Phytophthora infestans (Pi) [45], and Fusarium sulphureum (Fs) [46].…”