Galectin-3 impacts Cryptococcus neoformans infection through direct antifungal effects

Almeida, Fausto; Wolf, Julie M.; Silva, Thiago Aparecido da; DeLeon-Rodriguez, Carlos M.; Rezende, Caroline Patini; Pessoni, André Moreira; Fernandes, Fabrício Freitas; Silva‐Rocha, Rafael; Martínez, Roberto; Rodrigues, Márcio L.; Roque-Barreira, Maria Cristina; Casadevall, Arturo

doi:10.1038/s41467-017-02126-7

Cited by 78 publications

(107 citation statements)

References 51 publications

(67 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…More recently, it was also shown to play a role in C. neoformans infection, as galectin-3-deficient mice were found to be more susceptible to cryptococcosis than wild-type mice. In addition, galectin-3 was able to inhibit fungal growth and exert a direct lytic effect on C. neoformans extracellular vesicles protecting the host from potential fungal virulence factors carried in these vesicles [49]. …”

Section: Additional Mannose-binding Lectinsmentioning

confidence: 99%

Antifungal Innate Immunity: A Perspective from the Last 10 Years

Salazar¹,

Brown²

2018

J Innate Immun

View full text Add to dashboard Cite

Fungal pathogens can rarely cause diseases in immunocompetent individuals. However, commensal and normally nonpathogenic environmental fungi can cause life-threatening infections in immunocompromised individuals. Over the last few decades, there has been a huge increase in the incidence of invasive opportunistic fungal infections along with a worrying increase in antifungal drug resistance. As a consequence, research focused on understanding the molecular and cellular basis of antifungal immunity has expanded tremendously in the last few years. This review will provide an overview of the most exciting recent advances in innate antifungal immunity, discoveries that are helping to pave the way for the development of new strategies that are desperately needed to combat these devastating diseases.

show abstract

Section: Additional Mannose-binding Lectinsmentioning

confidence: 99%

Antifungal Innate Immunity: A Perspective from the Last 10 Years

Salazar¹,

Brown²

2018

J Innate Immun

View full text Add to dashboard Cite

show abstract

“…5). The earli- exists in vivo (153). Indeed, galectin-3 KO mice experience increased C. neoformans burden and mortality following infection.…”

Section: G Galectin Interactions With Fungimentioning

confidence: 99%

Galectin Regulation of Host Microbial Interactions

Robinson

Arthur

Stowell

2018

TIGG

View full text Add to dashboard Cite

Galectins regulate a wide variety of biological processes. However, one of the earliest and most common galectin activities is likely their ability to recognize microbes. Galectin binding to microbes can result in direct microbial killing and activation of host immunity, eventually enhancing the ability of a host to eliminate microbes. However, microbes appear to have also evolved the ability to utilize galectins to enhance host attachment, ultimately leading to increased risk for infection. The ability of galectins to directly engage microbes, coupled with their role in regulating host immune function, positions these carbohydrate binding proteins as key factors that can dictate the consequence of microbial exposure. In this way, galectins represent a highly pleiotropic protein family involved in the regulation of a broad range of host-microbial interactions.

show abstract

“…Fungal EVs carry several virulence factors and other important molecules, contributing to fungal pathogenicity and host immunomodulation (9–14). Since EVs plays significant roles in the host-pathogen relationship, the vesicular stability is important to ensure suitable delivery of their cargo into host cells (13, 15).…”

Section: Introductionmentioning

confidence: 99%

“…These interactions commonly affect the microorganism pathogenesis, the host immune response or the success of intracellular parasitism (16–19). Recently, we have reported that Galectin-3 (Gal-3), a β-galactoside-binding animal lectin, plays significant roles in cryptococcal infection (13). Gal-3 interferes the C. neoformans infection, inhibiting C. neoformans growth and promoting vesicle disruption (13).…”

Section: Introductionmentioning

confidence: 99%

“…Recently, we have reported that Galectin-3 (Gal-3), a β-galactoside-binding animal lectin, plays significant roles in cryptococcal infection (13). Gal-3 interferes the C. neoformans infection, inhibiting C. neoformans growth and promoting vesicle disruption (13). Also, Gal-3 has been reported to influence the outcome of other mycoses, such as Candida albicans (20) and Histoplasma capsulatum (21).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Galectin-3 inhibits Paracoccidioides brasiliensis growth and impacts paracoccidioidomycosis through multiple mechanisms

Hatanaka

Rezende

Moreno

et al. 2019

Preprint

Self Cite

View full text Add to dashboard Cite

The thermodimorphic pathogenic fungi Paracoccidioides brasiliensis and Paracoccioidioides lutzii are the etiologic causes of paracoccidioidomycosis (PCM), the most prevalent systemic mycosis in Latin America. Galectin-3 (Gal-3), an animal β-galactoside-binding protein, modulates important roles during microbial infections, such as triggering a Th2-polarized immune response in PCM. Herein, we demonstrate that Gal-3 also plays other important roles in P. brasiliensis infection. We verified Gal-3 levels are upregulated in human and mice infections and establish that Gal-3 inhibits P. brasiliensis growth by inhibiting budding. Furthermore, Gal-3 affects disruption and internalization of extracellular vesicles (EV) from P. brasiliensis by macrophages. Our results suggest important roles for Gal-3 in P. brasiliensis infection, indicating that increased Gal-3 production during P. brasiliensis infection may account for affecting the fungal growth and EV stability, promoting a benefic course of experimental PCM.IMPORTANCEParacoccidiodomycosis (PCM) is the most prevalent systemic mycosis in Latin America. Although the immune mechanisms to control PCM are still not fully understood, several events of the host innate and adaptive immunity are crucial to determine the progress of the infection. Mammalian β-galactoside-binding protein Galectin-3 (Gal-3) plays significant roles during microbial infections, and has been studied for its immunomodulatory roles but it can also have direct antimicrobial effects. We asked whether this protein plays a role in P. brasiliensis. We report herein that Gal-3 indeed has direct effects on fungal pathogen, inhibiting fungal growth and reducing extracellular vesicles stability. Our results suggest a direct role for Gal-3 in P. brasiliensis infection, with beneficial effects for the mammalian host.

show abstract

Galectin-3 impacts Cryptococcus neoformans infection through direct antifungal effects

Cited by 78 publications

References 51 publications

Antifungal Innate Immunity: A Perspective from the Last 10 Years

Antifungal Innate Immunity: A Perspective from the Last 10 Years

Galectin Regulation of Host Microbial Interactions

Galectin-3 inhibits Paracoccidioides brasiliensis growth and impacts paracoccidioidomycosis through multiple mechanisms

Contact Info

Product

Resources

About