Systemic Israeli acute paralysis virus (IAPV) infection in bumblebees (Bombus terrestris) through feeding and injection

Wang, Haidong; Meeus, Ivan; Piot, Niels; Smagghe, Guy

doi:10.1016/j.jip.2017.11.015

Cited by 15 publications

(16 citation statements)

References 35 publications

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“…The former route of transmission, injection into the haemocoel, through V. destructor host feeding is thought to account for the huge increase in viral prevalence and intensity of infection of DWV in honeybees [75,76]. In support of this view, injection of another honeybee virus, Israeli acute paralysis virus, into B. terrestris led to systemic infection and rapid host death, whereas oral infection led to infection of the host gut in a dose-dependent manner and with more limited impact on host health [49].…”

Section: Discussionmentioning

confidence: 99%

“…Inoculation of B. terrestris workers by feeding was designed to test the likely route of viral spillover from honeybees at flowers in the field. Freshly emerged (24-48 h after eclosion) bumblebee workers were individually fed with 10 9 viral genome equivalents or the equivalent control solution devoid of virus (electronic supplementary material), a quantity inducing an acute infection [48,49]. Then bees were transferred to a new, autoclaved metal cage in small groups (5-10 bees per cage, grouped according to treatment).…”

Section: Bumblebees: Fed Inoculum Satiatedmentioning

confidence: 99%

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Experimental infection of bumblebees with honeybee-associated viruses: no direct fitness costs but potential future threats to novel wild bee hosts

et al. 2020

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Pathogen spillover represents an important cause of biodiversity decline. For wild bee species such as bumblebees, many of which are in decline, correlational data point towards viral spillover from managed honeybees as a potential cause. Yet, impacts of these viruses on wild bees are rarely evaluated. Here, in a series of highly controlled laboratory infection assays with well-characterized viral inocula, we show that three viral types isolated from honeybees (deformed wing virus genotype A, deformed wing virus genotype B and black queen cell virus) readily replicate within hosts of the bumblebee Bombus terrestris . Impacts of these honeybee-derived viruses - either injected or fed - on the mortality of B. terrestris workers were, however, negligible and probably dependent on host condition. Our results highlight the potential threat of viral spillover from honeybees to novel wild bee species, though they also underscore the importance of additional studies on this and other wild bee species under field-realistic conditions to evaluate whether pathogen spillover has a negative impact on wild bee individuals and population fitness.

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Section: Discussionmentioning

confidence: 99%

Section: Bumblebees: Fed Inoculum Satiatedmentioning

confidence: 99%

Experimental infection of bumblebees with honeybee-associated viruses: no direct fitness costs but potential future threats to novel wild bee hosts

et al. 2020

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“…Seeing the high diversity of parasites found in the honey bee-collected pollen and knowing that often high viral particle dosages are needed to establish oral infection 6,47 , we decided to focus on the study of the infection potential of parasites. In order to check for the infection potential of parasites within honey bee-collected pollen, we used a subset of pollen batches used for the prevalence study described above.…”

Section: Methodsmentioning

confidence: 99%

Honey bee-collected pollen is a potential source of Ascosphaera apis infection in managed bumble bees

Pereira

Meeus

Smagghe

2019

Sci Rep

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The trade of bumble bees started in the early nineties for pollinator-dependent greenhouse plants. Nowadays, its rearing and transport have received public attention, since managed bees can transfer pathogens to wild bee populations. Therefore, guaranteeing pathogen-free bumble bees is fundamental. The major protein source used in rearing facilities is honey bee-collected pollen. This can carry pathogens, however to date, solid data on the risk of this food source to the health of bumble bees is lacking. Here we performed a large pathogen screening of non-irradiated honey bee-collected pollen to discover particles infective to Bombus terrestris. We identified seven parasites (Apicystis bombi, Ascosphaera apis, Crithidia mellificae, Nosema ceranae, Paenibacillus larvae and two parasites resembling Nosema thomsoni and Microsporidium sp. Oise) and four viruses (CBPV, DWV, IAPV and SBV) in 17 pollen batches from two major European pollen source regions (Spain and Romania). Ascosphaera apis was capable of infecting bumble bees; the larvae showed similar symptoms to chalkbrood disease reported in honey bees. Bumble bee breeding facilities need to be cautious about the potential presence of this disease, which was originally reported in honey bees. Thorough diagnostic and control methods are needed, as risk of spillover to wild bee species is possible.

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“…Niu et al (2014) showed that injection with as low as 20 virus particles of IAPV originating from honey bees results in infection in B. terrestris. Similarly, Wang et al (2017) showed that injection of 500 virus particles of IAPV from honey bees results in an acute infection inducing rapid mortality in B. terrestris. The injection of SBPV particles, on the other hand, has a much less lethal effect on B. terrestris.…”

Section: Micro-injectionmentioning

confidence: 98%

Bee Viruses: Routes of Infection in Hymenoptera

et al. 2020

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Numerous studies have recently reported on the discovery of bee viruses in different arthropod species and their possible transmission routes, vastly increasing our understanding of these viruses and their distribution. Here, we review the current literature on the recent advances in understanding the transmission of viruses, both on the presence of bee viruses in Apis and non-Apis bee species and on the discovery of previously unknown bee viruses. The natural transmission of bee viruses will be discussed among different bee species and other insects. Finally, the research potential of in vivo (host organisms) and in vitro (cell lines) serial passages of bee viruses is discussed, from the perspective of the host-virus landscape changes and potential transmission routes for emerging bee virus infections.

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Systemic Israeli acute paralysis virus (IAPV) infection in bumblebees (Bombus terrestris) through feeding and injection

Cited by 15 publications

References 35 publications

Experimental infection of bumblebees with honeybee-associated viruses: no direct fitness costs but potential future threats to novel wild bee hosts

Experimental infection of bumblebees with honeybee-associated viruses: no direct fitness costs but potential future threats to novel wild bee hosts

Honey bee-collected pollen is a potential source of Ascosphaera apis infection in managed bumble bees

Bee Viruses: Routes of Infection in Hymenoptera

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