Subcutaneous white adipocytes express a light sensitive signaling pathway mediated via a melanopsin/TRPC channel axis

Ondrusova, Katarina; Fatehi, Mohammad; Barr, Amy; Czarnecka, Zofia; Long, Wentong; Suzuki, Kunimasa; Campbell, S.; Philippaert, Koenraad; Hubert, Matthew; Tredget, Edward E.; Kwan, Peter; Touret, Nicolas; Wabitsch, Martin; Lee, Kevin Y.; Light, Peter E.

doi:10.1038/s41598-017-16689-4

Cited by 43 publications

(48 citation statements)

References 45 publications

(47 reference statements)

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“…In humans, it has been reported that OPN4 is expressed in subcutaneous white adipose tissue and in blood vessels as a peripheral tissue and that lipolysis is facilitated and blood vessels are dilated by blue light irradiation (Ondrusoval et al, ; Sikka et al, ). These suggest that OPN4 functions even in peripheral tissues, and thus, it is not surprising that OPN4 is expressed and has some functions in skin tissue.…”

Section: Discussionmentioning

confidence: 99%

OPN4 belongs to the photosensitive system of the human skin

et al. 2020

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The human skin has previously been described to be affected by light; however, the underlying mechanism remains unknown. OPN4 (melanopsin) expression was first identified in the skin of amphibians; however, whether it is also expressed and functioned in the human skin has not yet been identified. Here, we show that OPN4 was expressed in the human skin tissue and cultures of isolated keratinocytes, melanocytes and fibroblasts. Additionally, Ca2+ influx in vitro and ex vivo and phosphorylation of extracellular signal‐regulated kinases 1/2 in human fibroblasts were observed by stimulation of blue light irradiation. Notably, our findings showed that this Ca2+ influx and phosphorylation of extracellular signal‐regulated kinases 1/2 are promoted in an intensity‐dependent manner, indicating that the light signal is converted to an intracellular signal via OPN4 in the human skin. Overall, in this study we showed that the human skin functions as a photoreceptor by demonstrating that in human skin, the photoreceptive protein was expressed, and photoreception was conducted via photoreceptive protein.

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Section: Discussionmentioning

confidence: 99%

OPN4 belongs to the photosensitive system of the human skin

et al. 2020

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“…To date, however, there are only a few suggestions of extraocular light reception in mammals. Notably, it has been shown that non-visual opsins function within cultured adipocytes (Ondrusova et al, 2017), and within cultured skin cells (Regazzetti et al, 2018) and can also mediate a vasorelaxation response (Sikka et al, 2014(Sikka et al, , 2016.…”

Section: Discussionmentioning

confidence: 99%

“…It has previously been suggested that melanopsin (OPN4) can mediate light responses in cultured primary adipocytes (Ondrusova et al, 2017). This suggested the interesting possibility that OPN3 and OPN4, both blue light responsive, might act in concert to regulate white adipocyte lipolysis.…”

Section: White Adipocyte Opn3 Is Required For Normal Lipolysis Duringmentioning

confidence: 99%

“…Evidence for the function of these signals is limited to date but includes a possible role for OPN4 in acutely-regulated dilation of blood vessels (Sikka et al, 2014(Sikka et al, , 2016 and in the modulation of melanocyte pigmentation (de Assis et al, 2018;Ozdeslik et al, 2019). It has also been suggested that adipocyte function might be modulated by light stimulation of OPN4 (Ondrusova et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Adaptive thermogenesis in mice requires adipocyte light-sensing via Opsin 3

Nayak

Vemaraju

Zhang

et al. 2019

Preprint

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Almost all life forms can decode light information for adaptive advantage. Examples include the visual system, where photoreceptor signals are interpreted as images, and the circadian system, where light entrains a physiological clock. Here we describe a local, nonvisual light response in mice that employs encephalopsin (OPN3, a 480 nm, blue light responsive opsin) to regulate the function of adipocytes. Germ line null and adipocytespecific conditional null mice show a deficit in thermogenesis that is phenocopied in mice under blue-light deficient conditions. We show that blue light stimulation of adipocytes activates hormone sensitive lipase, the rate limiting enzyme in the lipolysis pathway, and that this is OPN3-dependent. Opn3 adipocyte conditional null mice also use reduced levels of fat mass when fasted and cold exposed further suggesting a lipolysis deficit. These data suggest the hypothesis that in mice, a local, OPN3-dependent light response in adipocytes is a mechanism for regulation of energy homeostasis.

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“…As mentioned above, very recently, the melanopsin presence was discovered in subcutaneous white adipocytes. In these cells, similar to ipRGCs, the signaling cascade is mediated by both TRPC and PLC as delivery of inhibitor of melanopsin (opsinamide), inhibitor of TRPC channels (clemizole) or of PLC (U73122) inhibited light‐induced current in vitro in these cells .…”

Section: Melanopsin‐regulated Signaling Pathwaymentioning

confidence: 99%

Regulation of Melanopsin Signaling: Key Interactions of the Nonvisual Photopigment

Stachurska

Sarna

2018

Photochem & Photobiology

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Melanopsin is a G protein-coupled receptor with a peak sensitivity in the blue part of the spectrum, which plays a key role in nonvisual light-mediated signaling. Recently, its importance in forming visual pathway as well as its role in blood vessels photorelaxation was also revealed. Melanopsin was discovered in 1998 in Xenopus leavis. Since then, the melanopsin presence was demonstrated across the species. The existence of two melanopsin genes (opn4m and opn4x) as well as melanopsin isoforms resulting from alternative splicing contributes to the variety in melanopsin-regulated processes. In this review, the diversity in melanopsin-induced signaling, regulation of melanopsin activity by phosphorylation and regulation of melanopsin mRNA are discussed.

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Subcutaneous white adipocytes express a light sensitive signaling pathway mediated via a melanopsin/TRPC channel axis

Cited by 43 publications

References 45 publications

OPN4 belongs to the photosensitive system of the human skin

OPN4 belongs to the photosensitive system of the human skin

Adaptive thermogenesis in mice requires adipocyte light-sensing via Opsin 3

Regulation of Melanopsin Signaling: Key Interactions of the Nonvisual Photopigment

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