Anti-inflammatory effects of<i>Artemisia scoparia</i>and its active constituent, 3,5-dicaffeoyl-epi-quinic acid against activated mast cells

Nam, Sun‐Young; Han, Ning; Rah, So-Young; Seo, Youngwan; Kim, Hyung-Min; Jeong, Hyun‐Ja

doi:10.1080/08923973.2017.1405438

Cited by 20 publications

(13 citation statements)

References 32 publications

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“…Recently, A. scoparia has received immense attention due to its potential therapeutic impacts on indigenous communities. This species has been reported to contain anti-viral [6], anti-cancer [7], anti-inflammatory [8], anti-allergic [9], anti-oxidant, anti-malarial, insecticidal [10], anti-microbial [11], anti-hypertensive [12], and anti-obesity [13] properties. This species also has renal-protective [5], hepato-protective [14], hypo-lipidemic [15], and urease inhibitory [16] properties.…”

Section: Introductionmentioning

confidence: 99%

Chloroplast Genome Sequence of Artemisia scoparia: Comparative Analyses and Screening of Mutational Hotspots

Iram

Hayat

Tahir

et al. 2019

Plants

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Artemisia L. is among the most diverse and medicinally important genera of the plant family Asteraceae. Discrepancies arise in the taxonomic classification of Artemisia due to the occurrence of multiple polyploidy events in separate lineages and its complex morphology. The discrepancies could be resolved by increasing the genomic resources. A. scoparia is one of the most medicinally important species in Artemisia. In this paper, we report the complete chloroplast genome sequence of Artemisia scoparia. The genome was 151,060 bp (base pairs), comprising a large single copy (82,834 bp) and small single copy (18,282 bp), separated by a pair of long inverted repeats (IRa and IRb: 24,972 bp each). We identified 114 unique genes, including four ribosomal RNAs, 30 transfer RNAs, and 80 protein-coding genes. We analysed the chloroplast genome features, including oligonucleotide repeats, microsatellites, amino acid frequencies, RNA editing sites, and codon usage. Transversion substitutions were twice as frequent as transition substitutions. Mutational hotspot loci included ccsA-ndhD, trnH-psbA, ndhG-ndhI, rps18-rpl20, and rps15-ycf1. These loci can be used to develop cost-effective and robust molecular markers for resolving the taxonomic discrepancies. The reconstructed phylogenetic tree supported previous findings of Artemisia as a monophyletic genus, sister to the genus Chrysanthemum, whereby A. scoparia appeared as sister to A. capillaris.

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Section: Introductionmentioning

confidence: 99%

Chloroplast Genome Sequence of Artemisia scoparia: Comparative Analyses and Screening of Mutational Hotspots

Iram

Hayat

Tahir

et al. 2019

Plants

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“…To summarize, adipogenesis experiments with SCO crude partitions (EA, H, and W) and with HPLC or FCPC fractions of EA provided highly consistent results demonstrating that the fractions containing predominantly prenylated coumaric acid derivatives, prenylated cinnamic acids and/or sesquiterpene lactones, 6-demethoxycapillarisin, polymethoxyflavones, and coumarin monoterpene ethers promoted adipogenesis in 3T3-L1 cells, while fractions containing chlorogenates and quercetin derivatives did not. A compound detected in our inactive fractions, 3,5-dicaffeoylquinic acid (peak 5), has previously been isolated from A. scoparia and shown to have anti-inflammatory effects in activated mast cells (19). To determine whether it could regulate adipogenesis in our experimental conditions, we treated differentiating 3T3-L1 cells with commercially obtained 3,5-DCQA and did not observe any effects on lipid accumulation or adipogenic gene expression (Figure 6).…”

Section: Resultsmentioning

confidence: 93%

Distinct Fractions of an Artemisia scoparia Extract Contain Compounds With Novel Adipogenic Bioactivity

et al. 2019

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Adipocytes are important players in metabolic health and disease, and disruption of adipocyte development or function contributes to metabolic dysregulation. Hence, adipocytes are significant targets for therapeutic intervention in obesity and metabolic syndrome. Plants have long been sources for bioactive compounds and drugs. In previous studies, we screened botanical extracts for effects on adipogenesis in vitro and discovered that an ethanolic extract of Artemisia scoparia (SCO) could promote adipocyte differentiation. To follow up on these studies, we have used various separation methods to identify the compound(s) responsible for SCO's adipogenic properties. Fractions and subfractions of SCO were tested for effects on lipid accumulation and adipogenic gene expression in differentiating 3T3-L1 adipocytes. Fractions were also analyzed by Ultra Performance Liquid Chromatography- Mass Spectrometry (UPLC-MS), and resulting peaks were putatively identified through high resolution, high mass accuracy mass spectrometry, literature data, and available natural products databases. The inactive fractions contained mostly quercetin derivatives and chlorogenates, including chlorogenic acid and 3,5-dicaffeoylquinic acid, which had no effects on adipogenesis when tested individually, thus ruling them out as pro-adipogenic bioactives in SCO. Based on these studies we have putatively identified the principal constituents in SCO fractions and subfractions that promoted adipocyte development and fat cell gene expression as prenylated coumaric acids, coumarin monoterpene ethers, 6-demethoxycapillarisin and two polymethoxyflavones.

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“…scoparia has a well-documented history of medicinal use (12,13), and it has been shown to have a wide range of effects in disease models related to Alzheimer disease, renal oxidative stress, hepatotoxicity, hypertension, and others (14)(15)(16)(17). More specifically, anti-inflammatory effects of A. scoparia have been described in a wide range of cell types and contexts (18)(19)(20). In adipose tissue, TNFα secreted from resident macrophages is a principal mediator of obesity-associated inflammation (6,21,22).…”

Section: Original Articlementioning

confidence: 99%

Mechanisms of Artemisia scoparia’s Anti‐Inflammatory Activity in Cultured Adipocytes, Macrophages, and Pancreatic β‐Cells

Boudreau

Burke

Collier

et al. 2020

Obesity

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Objective An ethanolic extract of Artemisia scoparia (SCO) improves adipose tissue function and reduces negative metabolic consequences of high‐fat feeding. A. scoparia has a long history of medicinal use across Asia and has anti‐inflammatory effects in various cell types and disease models. The objective of the current study was to investigate SCO’s effects on inflammation in cells relevant to metabolic health. Methods Inflammatory responses were assayed in cultured adipocytes, macrophages, and insulinoma cells by quantitative polymerase chain reaction, immunoblotting, and NF‐κB reporter assays. Results In tumor necrosis factor α–treated adipocytes, SCO mitigated ERK and NF‐κB signaling as well as transcriptional responses but had no effect on fatty acid–binding protein 4 secretion. SCO also reduced levels of deleted in breast cancer 1 protein in adipocytes and inhibited inflammatory gene expression in stimulated macrophages. Finally, in pancreatic β‐cells, SCO decreased NF‐κB–responsive promoter activity induced by IL‐1β treatment. Conclusions SCO’s ability to promote adipocyte development and function is thought to mediate its insulin‐sensitizing actions in vivo. Our findings that SCO inhibits inflammatory responses through at least two distinct signaling pathways (ERK and NF‐κB) in three cell types known to contribute to metabolic disease reveal that SCO may act more broadly than previously thought to improve metabolic health.

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Anti-inflammatory effects ofArtemisia scopariaand its active constituent, 3,5-dicaffeoyl-epi-quinic acid against activated mast cells

Abstract: Therefore, these results indicated that AS and its active compound, DEQA may improve mast cell-mediated inflammatory diseases.

Cited by 20 publications

References 32 publications

Chloroplast Genome Sequence of Artemisia scoparia: Comparative Analyses and Screening of Mutational Hotspots

Chloroplast Genome Sequence of Artemisia scoparia: Comparative Analyses and Screening of Mutational Hotspots

Distinct Fractions of an Artemisia scoparia Extract Contain Compounds With Novel Adipogenic Bioactivity

Mechanisms of Artemisia scoparia’s Anti‐Inflammatory Activity in Cultured Adipocytes, Macrophages, and Pancreatic β‐Cells

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