Protective cerebrovascular effects of hydroxysafflor yellow A (HSYA) on ischemic stroke

Sun, Yang; Xu, Dong-ping; Qin, Zhen; Wang, Peng‐Yuan; Hu, Bo‐Han; Yu, Jian-Guang; Zhao, Yong; Cai, Ben; Chen, Yong-Ling; Lü, Min; Liu, Jianguo; Liu, Xia

doi:10.1016/j.ejphar.2017.11.033

Cited by 56 publications

(43 citation statements)

References 33 publications

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“…Hydroxysafflor yellow A (HSYA) is a bioactive component of the dried flower of Honghua ( Figure 1) (Hui et al, 2018). Studies have shown that DSS, SAA, SAB, and HSYA have therapeutic effects on cardiovascular and CVDs, through anti-inflammatory, anti-oxidative activities, etc (Fan et al, 2017;Feng et al, 2017;Xu et al, 2017;Sun et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Comparative Pharmacokinetics of Hydrophilic Components in Salvia miltiorrhiza Bge. and Carthamus tinctorius L. in Rats That Underwent Cerebral Ischemia Reperfusion Using an HPLC-DAD Method

Zhao

Chen

et al. 2020

Front. Pharmacol.

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Background: In China, the combination of herb Salvia miltiorrhiza Bge. (Danshen) and Carthamus tinctorius L. (Honghua) is an effective treatment for stroke. A previous study showed that the combination of four herbal components: danshensu (DSS), hydroxysafflor yellow A (HSYA), salvianolic acid A (SAA), and salvianolic acid B (SAB) was effective for treatment of cerebral ischemia-reperfusion (I/R) injury in rats. However, the pharmacokinetic characteristics of this formula require further investigation. The present study investigated the pharmacokinetic differences between each component of in two formulas in cerebral I/R injury rats. The influencing factors may affect the compatibility of components were analyzed.Methods: Focal cerebral I/R was induced by middle cerebral artery occlusion (MCAO). Rats that underwent MCAO were randomly divided into two groups and administered treatments through the tail vein. Blood samples were collected at predetermined time points following administration. The concentrations of DSS, HSYA, SAB, and SAA in rat plasma were determined using HPLC-DAD, and the main pharmacokinetic parameters were calculated. Pharmacokinetic parameters were calculated using DAS 3.2.6 software and SPSS 23.0 statistical analysis software.Results: Our results showed that DSS, HSYA, SAB, and SAA in MCAO model rats had statistically significant differences in two formulas. For DSS and SAA, pharmacokinetic parameters with statistically significant differences including AUC (0−t) , AUMC (0−t) , MRT (0−t) , VRT (0−t) , t 1/2z , V z , CL z , and C max (P < 0.01). For HSYA, significant differences in the parameters including AUC (0−t) , AUMC (0−t) , MRT (0−t) , VRT (0−t) (P < 0.01), CL z and C max (P < 0.05). Conclusion:The difference in pharmacokinetic parameters in response to each component may have been due to differences in the dosages of the components (HSYA, SAA, SAB) and the compatibility of components. Meanwhile, there were many Frontiers in Pharmacology | www.frontiersin.org January 2020 | Volume 10 | Article 1598 1

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Section: Introductionmentioning

confidence: 99%

Comparative Pharmacokinetics of Hydrophilic Components in Salvia miltiorrhiza Bge. and Carthamus tinctorius L. in Rats That Underwent Cerebral Ischemia Reperfusion Using an HPLC-DAD Method

Zhao

Chen

et al. 2020

Front. Pharmacol.

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“…The effects of temperature (40,50,60,70, and 80 • C), extraction time (20,30,40,50, and 60 min), solvent-to-material ratio (10,12,14,16, and 18 mL/g), and the extraction power (40,80,120,160, and 200 W) were investigated for the effect on the extraction of HSYA and AHSYB. The results demonstrated that the yield of HSYA and AHSYB were increased between temperatures of 20 to 70 • C, and the values of the extraction yield reached a maximum at a temperature of 70 • C. Therefore, the temperatures of 60, 70, and 80 • C were selected for the RSM experiment.…”

Section: Single-factor Experimentsmentioning

confidence: 99%

“…It is usually applied in food coloring additives and in the manufacture of health supplements as a safe and natural added component [10]. In particular, it has been shown to be effective in the treatment of cancer, cardiovascular diseases, cerebrovascular diseases, Alzheimer's disease, and ischemia/reperfusion-induced acute kidney injury [11][12][13][14]. Therefore, HSYA is extensively used in the field of pharmacology.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Optimization of the Ultrasonic Extraction Method and Determination of the Antioxidant Activities of Hydroxysafflor Yellow A and Anhydrosafflor Yellow B from Safflower Using a Response Surface Methodology

Zhang

Jin

et al. 2020

Molecules

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An evaluation of the ultrasonic extraction process and the antioxidant activities of hydroxysafflor yellow A (HSYA) and anhydrosafflor yellow B (AHSYB) from safflower are presented herein. Using response surface methodology (RSM), based on a four-factor-three-level Box–Behnken design (BBD), the extraction parameters, namely, temperature, extraction time, solvent-to-material ratio, and extraction power, were optimized for maximizing the yields of HSYA and AHSYB. The maximum yield was obtained at a temperature of 66 °C with an extraction time of 36 min, solvent-to-material ratio of 16 mL/g, and the extraction power of 150 W, which was adjusted according to the actual conditions. The HSYA and AHSYB contents were determined using high performance liquid chromatography (HPLC). The yield and the comprehensive evaluation value of HSYA and AHSYB were calculated. The antioxidant activities of the extracts were determined using a ferric reducing antioxidant power (FRAP) kit and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. The results suggested that the safflower extracts possessed obvious ferric reducing and DPPH radical scavenging activities. The antioxidant activity increased with increasing concentration. The results suggested that optimizing the conditions of ultrasonic extraction using RSM can significantly increase the yields of HSYA and AHSYB from safflower. The safflower extracts showed better antioxidant activity. This study can encourage future research on cardiovascular and cerebrovascular diseases.

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“…All identi ed compounds were subjected to ADME screening, among compounds below these requirements, six (Hydroxysa or yellow A, AstragalosideIV, Ferulic acid, Ligustrazine, Z-ligustilide, Linoleic acid) were considered bioactive compounds. These compounds are the major components of BYHWD, and their effects on cerebral ischemia is have been investigated previously [11][12][13][14][15][16]. Remove duplicate components and remove components with ambiguous targets.…”

Section: Compounds In Herbal Medicinesmentioning

confidence: 99%

Study on the Mechanism of Buyang Huanwu Decoction for the treatment of Ischemic Stroke Based on Network Pharmacology

Wang¹,

Lü²,

Cao³

et al. 2020

Preprint

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Background: Buyang Huanwu Decoction (BYHWD) is one of the representative prescriptions for tonifying qi and promoting blood circulation. This formula has been widely used in Chinese clinical practice for treatment and prevention of ischemic cerebral vascular diseases. However, the mechanism and active compounds of BYHWD used in clinical practice for ischemic stroke are not well understood. The purpose of this study was to understand the potential active components of BYHWD and further explore its mechanism of improving ischemic stroke. Methods: This study was based on network pharmacology and bioinformatics analysis. The compounds of BYHWD were obtained from public databases. Oral bioavailability as well as drug-likeness were screened by using absorption, distribution, metabolism, and excretion (ADME) criteria. Then, components of BYHWD, candidate targets of each component and known therapeutic targets of ischemic cerebral were collected. A network of compound-target genes and compound-ischemic cerebral was established by means of network pharmacology data sources. The enrichment of key targets and pathways was analyzed by using string database and DAVID database. In addition, we verified three key targets predicted by western blot analysis (IL6, VEGFA and HIF1A). Results: Network pharmacology analysis results of BYHWD identified 7 herbs, 42 compounds and 79 target genes associated to cerebral ischemia. The 10 key compounds were baicalein, beta-carotene, Baicalin, kaempferol, luteolin, quercetin, hydroxysafflor yellow A, isorhamnetin, Bifendate, formononetin,Calycosin, AstragalosideIV, Stigmasterol, sitosterol, Z-ligustilide, Dihydrocapsaicin. Core genes in this network were IL6, TNF, VEGFA, HIF1A, MAPK1, MAPK3, JUN, STAT3, IL1B and IL10. And pathways TNF, IL-17, Apoptosis, PI3K-Akt, Toll-like receptor, MAPK, NF-kappa B and HIF-1 signaling pathway, etc. related to ischemic stroke were identified. In vitro experiments, The results showed that compared with the control group (no treatment), BYHWD could significantly inhibit the expression of IL6 and increased the expression of HIF1A and VEGFA. Conclusions: Network pharmacology analysis can reveal close interactions between multi-components and multi-targets, and enhance our understanding of the potential effects of BYHWD in cerebral ischemia.

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Protective cerebrovascular effects of hydroxysafflor yellow A (HSYA) on ischemic stroke

Cited by 56 publications

References 33 publications

Comparative Pharmacokinetics of Hydrophilic Components in Salvia miltiorrhiza Bge. and Carthamus tinctorius L. in Rats That Underwent Cerebral Ischemia Reperfusion Using an HPLC-DAD Method

Comparative Pharmacokinetics of Hydrophilic Components in Salvia miltiorrhiza Bge. and Carthamus tinctorius L. in Rats That Underwent Cerebral Ischemia Reperfusion Using an HPLC-DAD Method

Simultaneous Optimization of the Ultrasonic Extraction Method and Determination of the Antioxidant Activities of Hydroxysafflor Yellow A and Anhydrosafflor Yellow B from Safflower Using a Response Surface Methodology

Study on the Mechanism of Buyang Huanwu Decoction for the treatment of Ischemic Stroke Based on Network Pharmacology

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