Molecular modeling and simulation of three important components of Plant Pathogen Interaction cascade in Vigna mungo

Abstract

Plant pathogen interaction plays a great role in plant immunity. The regulation of various components of plant pathogen interactions is quite complicated and is very important in establishing relationship among components of this system. Yellow Mosaic Disease is common among legumes such as Vigna mungo. Mungbean Yellow Mosaic India Virus (MYMIV) and whitefly (Bemisia tabaci) is a vector causing the disease. Therefore, it is of interest to document the molecule models of three different components of Plant Pathogen interaction cascade- MAP kinase1, MAP kinase 2 and WRKY33 from V. mungo resistant to MYMIV. Both the MAP kinases were sequenced for this study while WRKY 33 was extracted and modeled from transcripts generated from two different transcriptome libraries, one set MYMIV- challenged, the other fed with aviruliferous whitefly. Post simulation studies revealed that MAPKs contained less percentage of disordered residues and were structurally more stable and than WRKY33.

Transcriptomic and Metabolomic Analyses Reveal Key Metabolites, Pathways and Candidate Genes in Sophora davidii (Franch.) Skeels Seedlings Under Drought Stress

“…In the present study, the plant–pathogen interaction pathways in S. davidii seedlings were moderately and severely enriched in DEGs, indicating that the plant–pathogen defence system was rapidly activated. Under abiotic stress, WRKY TFs play an important role in plant transcriptional regulation and pathogen defence ( Singh et al, 2017 ) and have been widely reported in many plant studies ( Pandey et al, 2015 ; Zhang et al, 2018 ). In the present study, the expression of the WRKY33 gene was upregulated under MD and SD stress but did not change significantly under LD stress, indicating that WRKY33 may play a key role in the signal transduction and transcriptional regulation of the S. davidii defence response under MD and SD stress.…”