Aquicoccus porphyridii gen. nov., sp. nov., isolated from a small marine red alga, Porphyridium marinum

Abstract

A Gram-stain-negative, non-motile and aerobic bacterial strain, designated L1 8-17, was isolated from a marine alga, Porphyridium marinum, in South Korea. Cells of strain L1 8-17 were found to be oxidase- and catalase-positive cocci without flagella. Growth of strain L1 8-17 was observed at 20-40 °C (optimum, 37 °C), pH 6.0-10.0 (optimum, pH 7.0-8.0) and in the presence of 0-7 % (w/v) NaCl (optimum, 2-3 %). The isoprenoid quinone detected was only ubiquinone-10. Summed feature 8 (comprising C18 : 1ω7c/C18 : 1ω6c) and C16 : 0 were detected as major cellular fatty acids. The major polar lipids of strain L1 8-17 consisted of phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminolipid, an unidentified phospholipid and an unidentified lipid. The G+C content of the genomic DNA was 59.3 mol%. Strain L1 8-17 was most closely related to Marimonas arenosa CAU 1311, Tropicibacter naphthalenivorans C02 and Donghicola eburneus SW-277 with 96.68, 96.68 and 96.60 % 16S rRNA gene sequence similarities, respectively, but the strain formed a phylogenetic lineage clearly distinct from them within the family Rhodobacteraceae. On the basis of phenotypic, chemotaxonomic and molecular properties, strain L1 8-17 represents a novel genus of the family Rhodobacteraceae, for which the name Aquicoccus porphyridii gen. nov., sp. nov. is proposed. The type strain of the type species is L1 8-17 (KACC 18806=JCM 31543).

Carideicomes alvinocaridis gen. nov., sp. nov., a marine bacterium isolated from shrimp gill in a hydrothermal field of Okinawa Trough

“…The programs GET_HOMOLOGUES v3.0.3 [38] and RAxML v8.2.4 [39] were used for constructing the core genome tree of strain SCR17 T and other closely related species according to Lin et al [40], and the genome of Hyphomonas Table 1. Characteristics that differentiate strain SCR17 T from members of related genera Strains: 1, Carideicomes alvinocaridis SCR17 T (data from this study); 2, Aquicoccus porphyridii JCM 31543 T , data from Feng et al [13] unless indicated otherwise; 3, Roseivivax halodurans JCM 10272 T , data from Suzuki et al and Jung et al [14,41] unless indicated otherwise; 4, Roseivivax pacificus CGMCC 1.12410 T , data from Wu et al [15] unless indicated otherwise. +, Positive; −, negative; w, weakly positive reaction.…”

“… Aquicoccus porphyridii JCM 31543 T and Roseivivax halodurans JCM 10272 T , obtained from Japan Collection of Microorganisms (JCM), and Roseivivax pacificus CGMCC 1.12410 T , obtained from China General Microbiological Culture Collection Center (CGMCC), were selected as reference strains. Unless stated otherwise, all reference strains were grown under their optimal culture conditions [13–15].…”

“…S5), which are the same as A. porphyridii JCM 31543 T [13]. Strain SCR17 T and the three reference strains all contained PG and PE, while strain SCR17 T and R.…”

“…The core genome tree showed that strain SCR17 T formed a distinct lineage within the clade including the genera Ponticoccus and Roseivivax, similar to that found in phylogenetic trees reconstructed based on 16S rRNA genes that strain SCR17 T was closely related with the genus Roseivivax . The DNA G+C content of strain SCR17 T was 67.7 mol%, which was higher than for the genus Aquicoccus (59.3 %) [13], and within the range of the genus Roseivivax (64.1–69.8 mol%) [15, 41–45].…”

“…The polar lipids in strain SCR17 T mainly comprised phosphatidylcholine (PC), phosphatidylglycerol (PG) and phosphatidylethanolamine (PE), and the minor components included an unidentified aminolipid (AL), an unidentified phospholipid (PL) and an unidentified lipid (L) ( Fig. S5), which are the same as A. porphyridii JCM 31543 T [13]. Strain SCR17 T and the three reference strains all contained PG and PE, while strain SCR17 T and R. halodurans JCM 10272 T also contained PC and DPG as main polar lipids, respectively (Table S1, Fig S5) [15].…”