Effects of combined helium pre/post‐conditioning on the brain and heart in a rat resuscitation model

Abstract

Effects of combined helium pre/ post-conditioning on the brain and heart in a rat resuscitation model. Acta Anaesthesiologica Scandinavica 2018 doi: 10.1111/aas.13041Background: The noble gas helium induces cardio-and neuroprotection by pre-and post-conditioning. We investigated the effects of helium pre-and post-conditioning on the brain and heart in a rat resuscitation model. Methods: After approval by the Animal Care Committee, 96 Wistar rats underwent cardiac arrest for 6 min induced by ventricular fibrillation. Animals received 70% helium and 30% oxygen for 5 min before cardiac arrest and for 30 min after restoration of spontaneous circulation (ROSC). Control animals received 70% nitrogen and 30% oxygen. Hearts and brains were excised after 2, 4 h or 7 days. Neurological degeneration was evaluated using TUNEL and Nissl staining in the hippocampal CA-1 sector. Cognitive function after 7 days was detected with the tape removal test. .9] P = 0.023). Changes in Caveolin-3 expression in the membrane fraction and Hexokinase-II in the mitochondrial fraction were observed in the heart. Caveolin-1 expression of treated animals significantly differed from control animals in the membrane fraction of the heart and brain after ROSC. Conclusion: Treatment with helium reduced apoptosis in our resuscitation model. Differential expression levels of Caveolin-1, Caveolin-3 and Hexokinase II in the heart were found after helium pre-and post-conditioning. No beneficial effects were seen on neurofunctional outcome.

Helium-Induced Changes in Circulating Caveolin in Mice Suggest a Novel Mechanism of Cardiac Protection

“…Helium-induced cardioprotection was also observed in a rat in vivo study [4]. A recent study showed that seven days after combined He pre/post conditioning, Cav-3 levels in the rat heart decreased after an initial increase in a rat heart resuscitation model [24]. This might point to a translocation of Cav-3 in a time-dependent manner after He exposure.…”

“…Most importantly, caveolins have been shown to be critically involved in ischemic as well as volatile anesthetic-induced conditioning [17,18,19,20], and have been detected in mitochondria [10,21], where they modulate mitochondrial function [10,22,23]. It was previously shown that Cav-1 and Cav-3 levels are influenced by He pre and post conditioning in two different rat models [24,25]. Moreover, recently we were able to show that He preserves endothelial function by inducing the secretion of Cav-1 to the supernatant of human endothelial cells, with subsequent effects on endothelial permeability [26].…”

Inhalation of Heliox as a potential treatment for the ARDS caused by COVID-19

“…Helium induced changes in circulating caveolin in mice suggest a novel mechanism of cardiac protection [27]. In a rat model of resuscitation, one study examined the effects of helium pre-and postconditioning on the brain and heart [28]. This study showed that helium treatment resulted in signi cantly less apoptosis.…”

Adaptation of tape removal test for measurement of sensitivity in perineal area of rat

“…Since its development (Schallert et al, 1982), this test has been used in many studies and with various modifications. In most studies the tape is usually attached to the forepaws (Aehling et al, 2018;Albertsmeier et al, 2007;Schallert et al, 2000). However, as tested by Schallert et al (1982) it can be also used in other body parts including the snout (Fleming et al, 2013;Wang et al, 2016) or hindlimbs (Arakawa et al, 2014;Tsytsarev et al, 2017).…”

“…Later, it has been adapted for different central nervous system injuries or disorders. Most commonly it is used in brain injury models such as stroke (Duricki et al, 2016;Zhang et al, 2000), cardiac arrest (Aehling et al, 2018;Albertsmeier et al, 2007) or P s (Fleming et al, 2013;Schallert et al, 2000). However, it has also been used in several models of spinal cord injury (Fagoe et al, 2016;Onifer et al, 2005;Schallert et al, 2000).…”