“…For ChIP-seq analysis, libraries were prepared from AR- (C-terminal, IGBMC, #3299), GR- (C-terminal, IGBMC, #3249), H3K27ac- (Active Motif, 39133), H3K27me3- (Active motif, 39155), H3K4me3- (Abcam, 1012-100), H3K4me1- (Active Motif, 39297), Polr2- (Santa Cruz H-224, SC9001) and Ctcf- (Sigma-Aldrich, 07–729) immunoprecipitated DNA from skeletal muscle nuclear extracts as described ( 34 ). ChIP-seq libraries were sequenced with an Illumina Hiseq 4000 as paired-end 100 bp reads for Ctcf and H3K4me3 or single-end 50 bp reads for the other sequencing datasets, and mapped to the mm10 reference genome using Bowtie 2 ( 25 ).…”