Screening of tropically derived, multi-trait plant growth- promoting rhizobacteria and evaluation of corn and soybean colonization ability

Batista, Bruna Durante; Lacava, Paulo Teixeira; Ferrari, Andréa; Teixeira-Silva, Natalia de Sousa; Bonatelli, Maria Letícia; Tsui, Sarina; Mondin, Mateus; Kitajima, Elliot Watanabe; Pereira, José Odair; Azevedo, João Lúcio de; Quecine, Maria Carolina

doi:10.1016/j.micres.2017.09.007

Cited by 100 publications

(86 citation statements)

References 76 publications

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“…The test included a positive control, the bacterial strain EN303, and Pseudomonas oryzihabitans (Kuklinsky et al, 2004). This test is still a widely-used approach to perform bacteria screening for in vitro physiological potential, as assumed by many authors in recent works (Quecine et al, 2012;Oliveira et al, 2017;Batista et al, 2018;Leite et al, 2018;Rodriguez et al, 2018).…”

Section: Screening For Ppfm Bacteria Able To Fix Nitrogen In Vitromentioning

confidence: 99%

“…They can be characterized as beneficial when these microbes harbor important functions related to plant growth and development. During the last decades, the evolution of the in vitro cultivation drove many authors to isolate a high diversity of beneficial microorganisms directly from the environment and associated to host (Rodrigues et al, 2018;Batista et al, 2018). The necessity to understand the mechanisms involved in the interaction between plant and bacteria and the biotechnological potentials driving the sustainable crop cultivation, leads the bioprospection of specific microbial groups that develop a close interaction with the plant tissues (Dourado et al, 2012;Batista et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Differential niche occupation and the biotechnological potential of Methylobacterium species associated with sugarcane plants

Andrade¹,

Dias²,

Cotta³

et al. 2018

Afr. J. Microbiol. Res.

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This work highlighted a putative link between the physiological activity and genetic diversity of Methylobacterium species and the association with sugarcane roots and rhizoplane. In total, 40 isolates previously described as pink-pigmented facultative methylotrophic bacteria (PPFMs), were evaluated for their ability to fix nitrogen and solubilize inorganic phosphate, amylase and pectinase activity. This in vitro potential was positively correlated with the community isolated from the root tissues than those from the rhizoplane. Regarding the genomic fingerprinting, the (BOX-PCR) approach revealed a low similarity among the isolates, occurring sole 7 haplotypes harboring more than 70% of similarity among band patterns. These results revealed that the genomic fingerprinting of the isolates recovery from roots is different from the rhizoplane. Besides that, these haplotypes occurred on both sugarcane varieties. Using a phylogenetic sequencing approach based on the 16S rRNA gene, we observed a high abundance of sequences similar to Methylobacterium radiotolerans colonizing both plant tissue and sugarcane varieties were observed. Hence, it was suggested that the plant should select those Methylobacterium spp. with a high biotechnological potential to promote plant growth. Therefore, the bioprospection of specific endophytic bacterial groups comprise an important source of biotechnological potential to improve sugarcane growth and production.

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Section: Screening For Ppfm Bacteria Able To Fix Nitrogen In Vitromentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Differential niche occupation and the biotechnological potential of Methylobacterium species associated with sugarcane plants

Andrade¹,

Dias²,

Cotta³

et al. 2018

Afr. J. Microbiol. Res.

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“…Among other possible promising strains, we highlight Bacillus sp. RZ2MS9, a PGPB isolated from the rhizosphere of guarana (Paullinia cupana) that expressively promoted maize and soybean growth under greenhouse conditions (Batista et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…The maize inoculation with RZ2MS9 increased the dry weight of root in 136.9% compared to the noninoculated control. Furthermore, this PGPB in vitro assays was able to produce of indole acetic acid (IAA), siderophore, potential to biological nitrogen fixation and phosphate solubilization (Batista et al, 2018). These abilities were confirmed with the Bacillus sp.…”

Section: Introductionmentioning

confidence: 99%

Molecular mechanisms involved in the bacterial talking and maize growth promotion

Almeida¹

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Molecular mechanisms involved in the bacterial talking and maize growth promotion With the increase of agricultural production, there is an improvement in the use of mineral fertilizers, which may cause different environmental problems, besides the soil salinization. A possible alternative for reducing the application of these products is the use of plant growth-promoting bacteria (PGPB), that can be used alone or in co-inoculation, resulting in an alternative environmentally and economically feasible. Better results can be obtained if the interaction among bacteria-bacteria and bacteria-plant be elucidated, and strategy developed to optimize these interactions. Thus, the plant growth-promoting Bacillus sp. RZ2MS9, previous described as a potential PGPB in maize and soybean, was GFP-tagged and monitored alone and co-inoculated with Azospirillum brasilense (Ab-v5::pWM1013) during maize colonization. The interaction of tagged strains in maize were monitored by fluorescent microscopy (FM) and quantitative PCR (qPCR), demonstrating an endophytic behavior of Bacillus sp. RZ2MS9. Although the non-detection of Ab-v5::pWM1013, the co-inoculation resulted in the best increase in root and shoot dried weight, root volume and in root diameter, showing that inoculation with more than one strain can be a good choice to development of bio-fertilizers. One important system to bacterial interaction is the quorum sensing (QS). The QS is an important cell-cell communication system that allows bacterial cells to recognize their own population and modulate their gene expression. This, system is also involved in the interspecific communication, including other bacterial species and plants. In the other hand, enzymes able to detect and degrade these molecules evolved, the called quorum quenching (QQ) system, that has been evolved in some bacteria as competitive advantage for niches colonization. The aiiA gene, was one of the first gene related with the QQ in Bacillus. The aiiA was found in Bacillus sp. RZ2MS9 genome. Through construction of a new QQ biosensor, Agrobacterium tumefaciens At11006, and validated by A. tumefaciens NTL4, the ability of RZ2MS9 to degrade QS molecules was confirmed. The knockout of aiiA gene was performed using the CRISPR-Cas9 system, confirming this gene function. By these results, the influence of QQ system of Bacillus sp. RZ2MS9 during maize colonization and RZ2MS9-A. brasilense-maize can be better investigated, opens the possibility to better understand the role of QQ system in the interaction among PGPB and plants.

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“…An exhaustive review of the published literature on plant-PAB interactions points out growth and crop yield responses under drought or salinity conditions as the main focus of experimental investigations (i.e., Batista et al (2018); Etesami (2018); Bounaffaa et al (2018)). Much less is known about the N pathways and possible N signalings mediating interactions between the plant and its PAB.…”

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confidence: 99%

Ureide metabolism in plant-associated bacteria: purine plant-bacteria interactive scenarios under nitrogen deficiency

Izaguirre‐Mayoral

Lazarovits²,

Baral

2018

Plant Soil

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Background The erratic alterations in climate being experienced in agriculture, such as extended periods of drought or heavy rainfalls, are bringing increasing concerns about nitrogen (N) management. Even in highinput farming systems, unpredictable weather patterns can cause N deficiencies and result in nutrient losses that contribute to major pollution issues in groundwater, lakes, and even the oceans. Our present understanding of the beneficial interactions between N-deficientchallenged plants and plant-associated bacteria (PAB), mainly of the phyla Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria, is largely based on

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Screening of tropically derived, multi-trait plant growth- promoting rhizobacteria and evaluation of corn and soybean colonization ability

Cited by 100 publications

References 76 publications

Differential niche occupation and the biotechnological potential of Methylobacterium species associated with sugarcane plants

Differential niche occupation and the biotechnological potential of Methylobacterium species associated with sugarcane plants

Molecular mechanisms involved in the bacterial talking and maize growth promotion

Ureide metabolism in plant-associated bacteria: purine plant-bacteria interactive scenarios under nitrogen deficiency

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