Novel Recombinant Multiepitope Proteins for the Detection of anti-
            <i>Cryptococcus</i>
            Antibodies

Brandão, Rafael Melo Santos de Serpa; Faria, Angélica Rosa; Andrade, Hélida Monteiro de; Martins, Liline Maria Soares; Silva, Adalberto Socorro da; Monte, Semíramis Jamil Hadad do

doi:10.2217/fmb-2017-0184

Cited by 5 publications

(5 citation statements)

References 31 publications

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“…Brandão et al (2018) [ 62 ] conducted a study to develop a new RMP for the diagnosis of human cryptococcosis, caused by Cryptococcus spp. Authors selected epitopes through bioinformatics analyses to form four new RMPs, called recombinant multiepitope proteins A, B, C, and D. All genes were expressed in E. coli BL21(DE3) cells, and an in-house ELISA was used to evaluate their performance.…”

Section: Rmps Applied In Disease Diagnosismentioning

confidence: 99%

Recombinant multiepitope proteins expressed in Escherichia coli cells and their potential for immunodiagnosis

Gonçalves,

Ribeiro,

Resende

et al. 2024

Microb Cell Fact

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Recombinant multiepitope proteins (RMPs) are a promising alternative for application in diagnostic tests and, given their wide application in the most diverse diseases, this review article aims to survey the use of these antigens for diagnosis, as well as discuss the main points surrounding these antigens. RMPs usually consisting of linear, immunodominant, and phylogenetically conserved epitopes, has been applied in the experimental diagnosis of various human and animal diseases, such as leishmaniasis, brucellosis, cysticercosis, Chagas disease, hepatitis, leptospirosis, leprosy, filariasis, schistosomiasis, dengue, and COVID-19. The synthetic genes for these epitopes are joined to code a single RMP, either with spacers or fused, with different biochemical properties. The epitopes’ high density within the RMPs contributes to a high degree of sensitivity and specificity. The RMPs can also sidestep the need for multiple peptide synthesis or multiple recombinant proteins, reducing costs and enhancing the standardization conditions for immunoassays. Methods such as bioinformatics and circular dichroism have been widely applied in the development of new RMPs, helping to guide their construction and better understand their structure. Several RMPs have been expressed, mainly using the Escherichia coli expression system, highlighting the importance of these cells in the biotechnological field. In fact, technological advances in this area, offering a wide range of different strains to be used, make these cells the most widely used expression platform. RMPs have been experimentally used to diagnose a broad range of illnesses in the laboratory, suggesting they could also be useful for accurate diagnoses commercially. On this point, the RMP method offers a tempting substitute for the production of promising antigens used to assemble commercial diagnostic kits.

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Section: Rmps Applied In Disease Diagnosismentioning

confidence: 99%

Recombinant multiepitope proteins expressed in Escherichia coli cells and their potential for immunodiagnosis

Gonçalves,

Ribeiro,

Resende

et al. 2024

Microb Cell Fact

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“…A number of other serologic procedures have been developed and are reported to improve cryptococcosis diagnosis. The alternative assays are based upon recombinant multi-epitope proteins, specific monoclonal antibodies, and the fungal heat shock protein 70 [ 153 , 154 , 155 ]. These tests have great potential to be inserted into the array of diagnostic tests.…”

Section: Cryptococcosismentioning

confidence: 99%

Immunologic Diagnosis of Endemic Mycoses

Almeida‐Paes

Bernardes-Engemann

Motta

et al. 2022

JoF

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The endemic mycoses blastomycosis, coccidioidomycosis, histoplasmosis, paracoccidioidomycosis, cryptococcosis, sporotrichosis, talaromycosis, adiaspiromycosis, and emergomycosis are mostly caused by geographically limited thermally dimorphic fungi (except for cryptococcosis), and their diagnoses can be challenging. Usual laboratory methods involved in endemic mycoses diagnosis include microscopic examination and culture of biological samples; however, serologic, histopathologic, and molecular techniques have been implemented in the last few years for the diagnosis of these mycoses since the recovery and identification of their etiologic agents is time-consuming and lacks in sensitivity. In this review, we focus on the immunologic diagnostic methods related to antibody and antigen detection since their evidence is presumptive diagnosis, and in some mycoses, such as cryptococcosis, it is definitive diagnosis.

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“…There are other studies that synthesize multi-epitopes into recombinant proteins for detection, including C. albicans [ 170 ], Cryptococcus spp. [ 171 ], and Pneumocystis jirovecii [ 172 , 173 ].…”

Section: Applications In Diagnosismentioning

confidence: 99%

A Promising Tool in Serological Diagnosis: Current Research Progress of Antigenic Epitopes in Infectious Diseases

et al. 2022

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Infectious diseases, caused by various pathogens in the clinic, threaten the safety of human life, are harmful to physical and mental health, and also increase economic burdens on society. Infections are a complex mechanism of interaction between pathogenic microorganisms and their host. Identification of the causative agent of the infection is vital for the diagnosis and treatment of diseases. Etiological laboratory diagnostic tests are therefore essential to identify pathogens. However, due to its rapidity and automation, the serological diagnostic test is among the methods of great significance for the diagnosis of infections with the basis of detecting antigens or antibodies in body fluids clinically. Epitopes, as a special chemical group that determines the specificity of antigens and the basic unit of inducing immune responses, play an important role in the study of immune responses. Identifying the epitopes of a pathogen may contribute to the development of a vaccine to prevent disease, the diagnosis of the corresponding disease, and the determination of different stages of the disease. Moreover, both the preparation of neutralizing antibodies based on useful epitopes and the assembly of several associated epitopes can be used in the treatment of disease. Epitopes can be divided into B cell epitopes and T cell epitopes; B cell epitopes stimulate the body to produce antibodies and are therefore commonly used as targets for the design of serological diagnostic experiments. Meanwhile, epitopes can fall into two possible categories: linear and conformational. This article reviews the role of B cell epitopes in the clinical diagnosis of infectious diseases.

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Novel Recombinant Multiepitope Proteins for the Detection of anti- Cryptococcus Antibodies

Abstract: The multiepitope proteins were shown to be potential antigens for the diagnosis of cryptococcosis in an attempt to detect anti-Cryptococcus antibodies.

Cited by 5 publications

References 31 publications

Recombinant multiepitope proteins expressed in Escherichia coli cells and their potential for immunodiagnosis

Recombinant multiepitope proteins expressed in Escherichia coli cells and their potential for immunodiagnosis

Immunologic Diagnosis of Endemic Mycoses

A Promising Tool in Serological Diagnosis: Current Research Progress of Antigenic Epitopes in Infectious Diseases

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