Preparation and characterization of a novel molecularly imprinted polymer for the separation of glycyrrhizic acid

Abstract

Molecularly imprinted polymers of glycyrrhizic acid were prepared by solution polymerization using glycyrrhizic acid as the template molecule, N-vinypyrrolidone as functional monomer, N,N-methylene bisacrylamide as cross-linker and ascorbic acid and hydrogen peroxide as initiators. Focused on the adsorption capacity and separation degree of the polymer to glycyrrhizic acid, the effects of the monomers, crosslinker and initiators were investigated and optimized. Finally, the structure of the polymer was characterized by using Fourier transform infrared spectroscopy and scanning electron microscopy. To obtain objective results, non-imprinted molecular polymers prepared under the same conditions were also characterized. The adsorption quantity of the polymer was measured by high-performance liquid chromatography. Under the optimum conditions, the maximum adsorption capacity of glycyrrhizic acid approached 15 mg/g, and the separation degree was as high as 2.5. The adsorption kinetics could be well described by a pseudo-first-order model, while the thermodynamics of the adsorption process could be described by the Langmuir model.

Design of selective molecularly imprinted sorbent for the optimized solid‐phase extraction of S‐pramipexole from the model multicomponent sample of human urine

“…Common extraction protocols available for commercial sorbents are ineffective while applied for MIP because of the specific nature of such sorbents . Thus, the elaboration of new MIP material dedicated to unique analyte required to propose individually optimized extraction procedure and comprise additional novelty of our article .…”

Simultaneous quantitation of 13 active components in SimiaoYong'an decoction using high-performance liquid chromatography with diode array detection

“…It mainly consists of organic acids (such as ferulic acid) and volatile oils (such as ligustilide), which exert many pharmacological activities such as anti-thrombosis, anti-inflammatory, and antimicrobial [18][19][20]. Glycyrrhizae radix et rhizoma, used as supplementary herb to coordinate with other herbs, is mainly composed of flavonoids and saponins, and glycyrrhizic acid is the typical constituents with extensive pharmacological effects as well as high content in its water extract [21,22]. Based on the above, thirteen compounds, which have certain pharmacological effects and relatively high contents for quantitative analysis, were selected for the quality control of SYD: neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isochlorogenic acid B, isochlorogenic acid A and isochlorogenic acid C from Lonicerae japonicae flos, harpagide, angoroside C, harpagoside and cinnamic acid from Scrophulariae radix, ferulic acid and ligustilide from Angelicae sinensis radix, and glycyrrhizic acid from Glycyrrhizae radix et rhizoma.…”

Preparation and evaluation of temperature and magnetic dual‐responsive molecularly imprinted polymers for the specific enrichment of formononetin

“…Molecular imprinting technology is a predetermined and useful separation and purification method for select specific target molecules by simulating biological antibodies . Molecularly imprinted polymers (MIPs) are prepared by using MIT with tailor‐made recognition sites for a given analyte and its structural analogs . MIPs present high specificity, desired selectivity, thermal stability, as well as low costs and easy preparation, which make them ideal materials for separation science .…”