Complete Genome Sequence of Bacillus altitudinis Type Strain SGAir0031 Isolated from Tropical Air Collected in Singapore

Vettath, Vineeth Kodengil; Junqueira, Ana Carolina M.; Uchida, Akira; Purbojati, Rikky W.; Houghton, James N. I.; Chénard, Caroline; Drautz–Moses, Daniela I.; Wong, Anthony; Kolundžija, Sandra; Clare, Megan E.; Lau, Kenny J X; Gaultier, Nicolas E.; Heinle, Cassie E.; Premkrishnan, Balakrishnan N. V.; Gusareva, Elena S.; Acerbi, Enzo; Yang, Liang; Schuster, Stephan C.

doi:10.1128/genomea.01260-17

Cited by 9 publications

(4 citation statements)

References 13 publications

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“…From the biochemical characterization, it is evident that B. altitudinis RSP75 can utilize many polysaccharide components of plant cell walls such as cellulose, xylan, starch, and mannose. Our observations are in congruence with those of Vettath and coworkers who stated that many genes (875 out of 3820 protein-coding genes) in B. altitudinis encode for carbohydrate and protein metabolism [66]. Since mannan constitutes an important component of prebiotics for animal husbandry and nutritional supplements [67], B. altitudinis RSP75 could prove to be a promising candidate for mannan oligosaccharide-based dietary supplements of dairy and feedstock.…”

Section: Discussionsupporting

confidence: 91%

Valorization Potential of a Novel Bacterial Strain, Bacillus altitudinis RSP75, towards Lignocellulose Bioconversion: An Assessment of Symbiotic Bacteria from the Stored Grain Pest, Tribolium castaneum

et al. 2021

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Bioconversion of lignocellulose into renewable energy and commodity products faces a major obstacle of inefficient saccharification due to its recalcitrant structure. In nature, lignocellulose is efficiently degraded by some insects, including termites and beetles, potentially due to the contribution from symbiotic gut bacteria. To this end, the presented investigation reports the isolation and characterization of cellulolytic bacteria from the gut system of red flour beetle, Tribolium castaneum. Out of the 15 isolated bacteria, strain RSP75 showed the highest cellulolytic activities by forming a clearance zone of 28 mm in diameter with a hydrolytic capacity of ~4.7. The MALDI-TOF biotyping and 16S rRNA gene sequencing revealed that the strain RSP75 belongs to Bacillus altitudinis. Among the tested enzymes, B. altitudinis RSP75 showed maximum activity of 63.2 IU/mL extract for xylanase followed by β-glucosidase (47.1 ± 3 IU/mL extract) which were manifold higher than previously reported activities. The highest substrate degradation was achieved with wheat husk and corn cob powder which accounted for 69.2% and 54.5%, respectively. The scanning electron microscopy showed adhesion of the bacterial cells with the substrate which was further substantiated by FTIR analysis that depicted the absence of the characteristic cellulose bands at wave numbers 1247, 1375, and 1735 cm−1 due to hydrolysis by the bacterium. Furthermore, B. altitudinis RSP75 showed co-culturing competence with Saccharomyces cerevisiae for bioethanol production from lignocellulose as revealed by GC-MS analysis. The overall observations signify the gut of T. castaneum as a unique and impressive reservoir to prospect for lignocellulose-degrading bacteria that can have many biotechnological applications, including biofuels and biorefinery.

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Section: Discussionsupporting

confidence: 91%

Valorization Potential of a Novel Bacterial Strain, Bacillus altitudinis RSP75, towards Lignocellulose Bioconversion: An Assessment of Symbiotic Bacteria from the Stored Grain Pest, Tribolium castaneum

et al. 2021

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“…and Gram-positive Bacillus altitudinis , Staphylococcus aureus, and Brachybacterium rhamnosum (labeled as B1 to B6, Figure B). We select these bacteria because they are commonly found in numerous clinical and environmental settings. − We note that the bacteria specimens cannot be readily differentiated via visual SEM inspection, whereby Pantoea stewartii (B1, 1540 ± 257 nm), Enterobacter hormaechei (B2, 1583 ± 248 nm), Pseudomonas sp. (B3, 1688 ± 353 nm), and Bacillus altitudinis (B4, 1119 ± 126 nm) exhibit rod-like appearances with wide size distributions, measured along the major axis (Figure C).…”

Section: Resultsmentioning

confidence: 99%

Surface-Enhanced Raman Scattering-Based Surface Chemotaxonomy: Combining Bacteria Extracellular Matrices and Machine Learning for Rapid and Universal Species Identification

Leong,

Tan,

Han

et al. 2023

ACS Nano

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Rapid, universal, and accurate identification of bacteria in their natural states is necessary for on-site environmental monitoring and fundamental microbial research. Surface-enhanced Raman scattering (SERS) spectroscopy emerges as an attractive tool due to its molecule-specific spectral fingerprinting and multiplexing capabilities, as well as portability and speed of readout. Here, we develop a SERSbased surface chemotaxonomy that uses bacterial extracellular matrices (ECMs) as proxy biosignatures to hierarchically classify bacteria based on their shared surface biochemical characteristics to eventually identify six distinct bacterial species at >98% classification accuracy. Corroborating with in silico simulations, we establish a three-way inter-relation between the bacteria identity, their ECM surface characteristics, and their SERS spectral fingerprints. The SERS spectra effectively capture multitiered surface biochemical insights including ensemble surface characteristics, e.g., charge and biochemical profiles, and molecular-level information, e.g., types and numbers of functional groups. Our surface chemotaxonomy thus offers an orthogonal taxonomic definition to traditional classification methods and is achieved without gene amplification, biochemical testing, or specific biomarker recognition, which holds great promise for point-of-need applications and microbial research.

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“…In recent articles published in the journal Genome Announcements (GA), (now Microbiology Resource Announcements [MRA]) (1–5), the complete genome sequences of five strains of five species of bacteria were reported. The titles of the articles stated that the strains represent the respective “type strains” of the five species, although the articles do not present details about the processes of defining the type strains.…”

Section: Lettermentioning

confidence: 99%

Beware of False “Type Strain” Genome Sequences

Salvà‐Serra

Jaén‐Luchoro

Karlsson

et al. 2019

Microbiol Resour Announc

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Complete Genome Sequence of Bacillus altitudinis Type Strain SGAir0031 Isolated from Tropical Air Collected in Singapore

Cited by 9 publications

References 13 publications

Valorization Potential of a Novel Bacterial Strain, Bacillus altitudinis RSP75, towards Lignocellulose Bioconversion: An Assessment of Symbiotic Bacteria from the Stored Grain Pest, Tribolium castaneum

Valorization Potential of a Novel Bacterial Strain, Bacillus altitudinis RSP75, towards Lignocellulose Bioconversion: An Assessment of Symbiotic Bacteria from the Stored Grain Pest, Tribolium castaneum

Surface-Enhanced Raman Scattering-Based Surface Chemotaxonomy: Combining Bacteria Extracellular Matrices and Machine Learning for Rapid and Universal Species Identification

Beware of False “Type Strain” Genome Sequences

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