Retraction Note to: L-glutamine supplementation induces insulin resistance in adipose tissue and improves insulin signalling in liver and muscle of rats with diet-induced obesity

Prada, Patrícia O.; Hirabara, Sandro Massao; Souza, Cláudio Teodoro de; Schenka, André Almeida; Zecchin, Henrique Gottardello; Vassallo, José; Velloso, Lı́cio A.; Carneiro, Everardo M.; Carvalheira, José B.C.; Curi, Rui; Saad, Mário José Abdalla

doi:10.1007/s00125-017-4477-y

Cited by 16 publications

(20 citation statements)

References 48 publications

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“…However, the increase in proportion of Bacteroidetes (43% to 52%) after GLN treatment observed in Figure 1 occurs because, even though the total number of bacteria in both groups decreased, the number of Firmicutes in the GLN group decreased more sharply. Supplementation with GLN in obese rats was also associated with a reduction of the pro-inflammatory cytokines TNF-α and IL-6 in serum and in peripheral tissues (24,25),…”

Section: Studies a Highermentioning

confidence: 98%

Oral supplementation with l-glutamine alters gut microbiota of obese and overweight adults: A pilot study

et al. 2015

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Section: Studies a Highermentioning

confidence: 98%

Oral supplementation with l-glutamine alters gut microbiota of obese and overweight adults: A pilot study

et al. 2015

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“…Similarly, dietary arginine supplementation increased the activity of mTOR signaling 2 of 22 in skeletal muscle of neonatal pigs [10]. Glutamine may prevent protein hypercatabolism via inhibition of myostatin hyperexpression [11], and supplementation of glutamine to a high-fat diet improved insulin signaling in the muscle of rats [12]. In terms of lysine, however, which signaling pathways related to muscle protein accretion and carcass characteristics are regulated by its dietary supply is still unknown.…”

Section: Introductionmentioning

confidence: 99%

A Systems Biology Approach Using Transcriptomic Data Reveals Genes and Pathways in Porcine Skeletal Muscle Affected by Dietary Lysine

Wang

Feugang

Crenshaw

et al. 2017

Preprint

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Nine crossbred finishing barrows randomly assigned to 3 dietary treatments were used to investigate the effects of dietary lysine on muscle growth related metabolic and signaling pathways. Muscle samples were collected from the longissimus dorsi of individual pigs after feeding the lysinedeficient, lysine-adequate, or lysine-excess diet for 5 weeks, and the total RNA was extracted afterwards. Affymetrix Porcine Gene 1.0 ST Array was used to quantify the expression levels of 19,211 genes. A total of 674 transcripts were differentially expressed (P ≤ 0.05); 60 out of 131 transcripts (P ≤ 0.01) were annotated in the NetAffx database. Ingenuity pathway analysis showed that dietary lysine deficiency may lead to (1) increased muscle protein degradation via the ubiquitination pathway as indicated by the up-regulated DNAJA1, HSP90AB1 and UBE2B mRNA, (2) reduced muscle protein synthesis via the up-regulated RND3 and ZIC1 mRNA, (3) increased serine and glycine synthesis via the up-regulated PHGDH and PSPH mRNA, and (4) increased lipid accumulation via the up-regulated ME1, SCD, and CIDEC mRNA. Dietary lysine excess may lead to (1) decreased muscle protein degradation via the down-regulated DNAJA1, HSP90AA1, HSPH1, and UBE2D3 mRNA, and (2) reduced lipid biosynthesis via the down-regulated CFD and ME1 mRNA.

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“…Animal care and research protocols were in accordance with the principles and guidelines adopted by the Brazilian College of Animal Experimentation (COBEA) and this project was approved by the Ethical Committee for Animal Research of the Institute of Biomedical Sciences of the University of São Paulo. A group of mice receiving a standard rodent chow were compared with a group that received a high-fat diet with 55% calories from fat for 12 consecutive weeks, as previously described (22). Body weight was recorded weekly.…”

Section: Introductionmentioning

confidence: 99%

PAFR in adipose tissue macrophages is associated with anti-inflammatory phenotype and metabolic homoeostasis

et al. 2016

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PAFR in adipose tissue macrophages is associated with anti-inflammatory phenotype and metabolic homoeostasis. Clinical Science, 130(8), pp. 601-612. (doi:10.1042/cs20150538) This is the author's final accepted version.There may be differences between this version and the published version. You are advised to consult the publisher's version if you wish to cite from it.http://eprints.gla.ac.uk/120744/ cells. Blood monocytes of PAFRKO mice also exhibited a pro-inflammatory phenotype (increased frequency of Lys6C+.cells) and PAFR-ligands were detected in the serum of both PAFRKO and WT. Regarding metabolic parameters, compared to WT, PAFRKO mice had: i) higher weight gain andserum glucose concentration levels; ii) decreased insulin-stimulated glucose disappearance; iii) insulin resistance in the liver; iv) increased expression of Ldlr in the liver. In mice fed HFD, some of these changes were potentiated, particularly in the liver. Thus, it seems that endogenous ligands of PAFR are responsible for maintaining the anti-inflammatory profile of blood monocytes and adipose tissue macrophages under physiological conditions. In the absence of PAFR signaling, monocytes and macrophages acquire pro-inflammatory phenotype, resulting in adipose tissue inflammation and metabolic dysfunction. SummaryWe found an essential role of PAFR in adipose tissue macrophages. The PAFRdeficiency leads to infiltration of pro-inflammatory macrophages in the adipose tissue resulting in weight gain, reduced glucose tolerance, hepatic insulin resistance, followed by hepatic steatosis.Short Title: PAFR is associated with anti-inflammatory macrophages and glucose metabolism

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Retraction Note to: L-glutamine supplementation induces insulin resistance in adipose tissue and improves insulin signalling in liver and muscle of rats with diet-induced obesity

Cited by 16 publications

References 48 publications

Oral supplementation with l-glutamine alters gut microbiota of obese and overweight adults: A pilot study

Oral supplementation with l-glutamine alters gut microbiota of obese and overweight adults: A pilot study

A Systems Biology Approach Using Transcriptomic Data Reveals Genes and Pathways in Porcine Skeletal Muscle Affected by Dietary Lysine

PAFR in adipose tissue macrophages is associated with anti-inflammatory phenotype and metabolic homoeostasis

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