Effect of naturally occurring
 Wolbachia
 in
 Anopheles gambiae s.l.
 mosquitoes from Mali on
 Plasmodium falciparum
 malaria transmission

Gomes, Fabio M.; Hixson, Bretta; Tyner, Miles D W; Ramı́rez, José Luis; Canepa, Gaspar E.; Silva, Thiago Luiz Alves e; Molina-Cruz, Alvaro; Keïta, Moussa; Kané, Fousseyni; Traoré, Boïssé; Sogoba, Nafomon; Barillas‐Mury, Carolina

doi:10.1073/pnas.1716181114

Cited by 98 publications

(215 citation statements)

References 45 publications

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“…gambiae s.l. populations from Mali 40 and Burkina Faso 39 , w Anga-Ghana is more closely related to the Supergroup B strain of w Anga from Burkina Faso. Although a resident strain was detected in An.…”

Section: Resultsmentioning

confidence: 89%

“…gambiae s.s. and a single An. arabiensis from DRC through amplification of 16S rRNA fragments using two independent PCR assays 40, 54 , we were unable to obtain 16S sequences of sufficient quality to allow further analysis. The Wolbachia wsp gene has been evolving at a faster rate and provides more informative strain phylogenies 55 .…”

Section: Resultsmentioning

confidence: 91%

“…Wolbachia detection was first undertaken targeting three conserved Wolbachia genes previously shown to amplify a wide diversity of strains; 16S rDNA gene 40, 54 , Wolbachia surface protein ( wsp ) gene 55 and FtsZ cell cycle gene 56 . DNA extracted from a Drosophila melanogaster fly (infected with the w Mel strain of Wolbachia ) was used a positive control, in addition to no template controls (NTCs).…”

Section: Methodsmentioning

confidence: 99%

“…The 16S rDNA 54 and wsp 55 gene PCR reactions were carried out in a Bio-Rad T100 Thermal Cycler using standard cycling conditions and PCR products were separated and visualised using 2% E-Gel EX agarose gels (Invitrogen) with SYBR safe and an Invitrogen E-Gel iBase Real-Time Transilluminator. FtsZ 56 and 16S rDNA 40 gene real time PCR reactions were prepared using 5 μl of FastStart SYBR Green Master mix (Roche Diagnostics), a final concentration of 1 µM of each primer, 1 μl of PCR grade water and 2 μl template DNA, to a final reaction volume of 10 μl. Prepared reactions were run on a Roche LightCycler® 96 System for 15 minutes at 95°C, followed by 40 cycles of 95°C for 15 seconds and 58°C for 30 seconds.…”

Section: Methodsmentioning

confidence: 99%

“…gambiae s.l. populations in Burkina Faso 39 and Mali 40 , suggesting that Wolbachia may be more abundant in the An. gambiae complex across Sub-Saharan Africa.…”

Section: Introductionmentioning

confidence: 99%

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Novel Wolbachia strains in Anopheles malaria vectors from Sub-Saharan Africa

Jeffries¹,

Lawrence²,

Golovko³

et al. 2018

Wellcome Open Res

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Background: Wolbachia, a common insect endosymbiotic bacterium that can influence pathogen transmission and manipulate host reproduction, has historically been considered absent from the Anopheles (An.) genera, but has recently been found in An. gambiae s.l. populations. As there are numerous Anopheles species that have the capacity to transmit malaria, we analysed a range of species to determine Wolbachia prevalence rates, characterise novel Wolbachia strains and determine any correlation between the presence of Plasmodium, Wolbachia and the competing endosymbiotic bacterium Asaia. Methods: Anopheles adult mosquitoes were collected from five malaria-endemic countries: Guinea, Democratic Republic of the Congo (DRC), Ghana, Uganda and Madagascar, between 2013 and 2017. Molecular analysis of samples was undertaken using quantitative PCR, Sanger sequencing, Wolbachia multilocus sequence typing (MLST) and high-throughput amplicon sequencing of the bacterial 16S rRNA gene. Results: Novel Wolbachia strains were discovered in five species: An. coluzzii, An. gambiae s.s., An. arabiensis, An. moucheti and An. species ‘A’, increasing the number of Anopheles species known to be naturally infected. Variable prevalence rates in different locations were observed and novel strains were phylogenetically diverse, clustering with Wolbachia supergroup B strains. We also provide evidence for resident strain variants within An. species ‘A’. Wolbachia is the dominant member of the microbiome in An. moucheti and An. species ‘A’, but present at lower densities in An. coluzzii. Interestingly, no evidence of Wolbachia/Asaia co-infections was seen and Asaia infection densities were also shown to be variable and location dependent. Conclusions: The important discovery of novel Wolbachia strains in Anopheles provides greater insight into the prevalence of resident Wolbachia strains in diverse malaria vectors. Novel Wolbachia strains (particularly high-density strains) are ideal candidate strains for transinfection to create stable infections in other Anopheles mosquito species, which could be used for population replacement or suppression control strategies.

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Section: Resultsmentioning

confidence: 89%

Section: Resultsmentioning

confidence: 91%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…gambiae s.l. populations in Burkina Faso 39 and Mali 40 , suggesting that Wolbachia may be more abundant in the An. gambiae complex across Sub-Saharan Africa.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Novel Wolbachia strains in Anopheles malaria vectors from Sub-Saharan Africa

Jeffries¹,

Lawrence²,

Golovko³

et al. 2018

Wellcome Open Res

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Wolbachia enhances insect‐specific flavivirus infection in Aedes aegypti mosquitoes

Amuzu

Tsyganov

Koh

et al. 2018

Ecology and Evolution

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Mosquitoes transmit a diverse group of human flaviviruses including West Nile, dengue, yellow fever, and Zika viruses. Mosquitoes are also naturally infected with insect‐specific flaviviruses (ISFs), a subgroup of the family not capable of infecting vertebrates. Although ISFs are not medically important, they are capable of altering the mosquito's susceptibility to flaviviruses and may alter host fitness. Wolbachia is an endosymbiotic bacterium of insects that when present in mosquitoes limits the replication of co‐infecting pathogens, including flaviviruses. Artificially created Wolbachia‐infected Aedes aegypti mosquitoes are being released into the wild in a series of trials around the globe with the hope of interrupting dengue and Zika virus transmission from mosquitoes to humans. Our work investigated the effect of Wolbachia on ISF infection in wild‐caught Ae. aegypti mosquitoes from field release zones. All field mosquitoes were screened for the presence of ISFs using general degenerate flavivirus primers and their PCR amplicons sequenced. ISFs were found to be common and widely distributed in Ae. aegypti populations. Field mosquitoes consistently had higher ISF infection rates and viral loads compared to laboratory colony material indicating that environmental conditions may modulate ISF infection in Ae. aegypti. Surprisingly, higher ISF infection rates and loads were found in Wolbachia‐infected mosquitoes compared to the Wolbachia‐free mosquitoes. Our findings demonstrate that the symbiont is capable of manipulating the mosquito virome and that Wolbachia‐mediated viral inhibition is not universal for flaviviruses. This may have implications for the Wolbachia‐based DENV control strategy if ISFs confer fitness effects or alter mosquito susceptibility to other flaviviruses.

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Detection of Natural Wolbachia Strains in Anopheles Mosquitoes

Walker

2023

Methods in Molecular Biology

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Effect of naturally occurring Wolbachia in Anopheles gambiae s.l. mosquitoes from Mali on Plasmodium falciparum malaria transmission

Cited by 98 publications

References 45 publications

Novel Wolbachia strains in Anopheles malaria vectors from Sub-Saharan Africa

Novel Wolbachia strains in Anopheles malaria vectors from Sub-Saharan Africa

Wolbachia enhances insect‐specific flavivirus infection in Aedes aegypti mosquitoes

Detection of Natural Wolbachia Strains in Anopheles Mosquitoes

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