Hepatoprotective effect of peony total glucosides and the underlying mechanisms in diabetic rats

Abstract

Context: Total glucosides of peony (TGP), compounds extracted from the dried roots of Paeonia lactiflora Pall, have been reported to have anti-inflammatory and antioxidative activities. However, the protective effect of TGP on liver injury and the underlying mechanisms remains unknown in diabetic rats.Objectives: Current study investigates prevention of liver injury by TGP in diabetic rats and its mechanism was related to the inhibition of endoplasmic reticulum stress (ERS).Materials and methods: Fifty adult male rats were randomly divided into: Normal group, diabetic group, TGP (50, 100 and 200 mg/kg/day) treatment groups (n = 10 per group). At the end of the 8th week, the liver was removed for biochemical and histological examinations.Results: Compared with the diabetic group, administration of TGP at doses of 50, 100 and 200 mg/kg significantly prevented the increase of hepatic fibrosis score (ED50 139.4 mg/kg). Compared with diabetic group, TGP at doses of 50, 100 and 200 mg/kg showed an inhibition on the increased macrophage infiltration. MCP-1 and TNF-α mRNA and protein expression were significantly increased in diabetic group compared with normal group; TGP administration caused significant reduction of high levels of MCP-1 and TNF-α mRNA as well as protein levels. Also, TGP at all doses showed an inhibition on the increased GRP78 levels, p-Perk levels and p-Eif2α levels in liver from diabetic group.Discussion and conclusions: Our results indicate that TGP has potential as a treatment for diabetic liver injury attenuating liver lipid accumulation and inflammation as well as ERS induced by diabetic condition.

Sclareol ameliorates liver injury by inhibiting nuclear factor-kappa B/NOD-like receptor protein 3-mediated inflammation and lipid metabolism disorder in diabetic mice

“…16,[41][42][43] Inflammation is a crucial contributor to the development of liver injury in diabetes by promoting the secretion of pro-inflammatory cytokines, such as TNFα and IL-6. 21,44,45 The increased inflammation, also observed in our study, is caused by the effect of T1DM on increased serum concentrations of IL-6 and TNFα. The beneficial effect of SCL on inflammation is consistent with previous finding, which showed that it effectively downregulated mRNA levels of TNF-α, IL-1β, and IL-6 in a hyperglycemia-induced mouse model of renal injury.…”

Metabolite profiles of Gansuibanxia decoction in rat plasma and urine by UHPLC‐Q‐TOF/MS analysis

“…These metabolites might have more therapeutic effects on body. In addition, glycosides such as triterpenoid saponins and monoterpene glycosides in GSBXD have anti‐inflammatory, anticancer, neuroprotective and hepatoprotective activities (Bisht et al, 2022; Hong et al, 2022; Xia et al, 2017). They predominantly undergo hydroxylation to generate aglycones by glycosidic bond cleavage, which can be absorbed into blood circulation more easily and exhibits better bioavailability (He et al, 2019).…”