A Quantitative Point-of-Need Assay for the Assessment of Vitamin D3 Deficiency

Vemulapati, Sasank; Rey, Elizabeth; O'Dell, Dakota; Mehta, Saurabh; Erickson, David

doi:10.1038/s41598-017-13044-5

Cited by 36 publications

(21 citation statements)

References 26 publications

(22 reference statements)

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“…Daily requirements, treatment guidelines and protocols, and monitoring strategies for LVD are unclear, variable, contradictory, and sometimes poorly-defined. Additionally, many laboratory methods are used to quantify 25-OH-D (e.g., liquid chromatography-tandem mass spectrometry, enzyme linked immunosorbent assay, chemiluminescence immunoassay, and new point-of-care assays [ 15 ]) resulting in notable intra- and inter-assay variability.…”

Section: Introductionmentioning

confidence: 99%

Clinical Management of Low Vitamin D: A Scoping Review of Physicians’ Practices

et al. 2018

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The role of vitamin D in the prevention and treatment of non-skeletal health issues has received significant media and research attention in recent years. Costs associated with clinical management of low vitamin D (LVD) have increased exponentially. However, no clear evidence supports vitamin D screening to improve health outcomes. Authoritative bodies and professional societies do not recommend population-wide vitamin D screening in community-dwelling adults who are asymptomatic or at low risk of LVD. To assess patterns of physicians’ management of LVD in this conflicting environment, we conducted a scoping review of three electronic databases and the gray literature. Thirty-eight records met inclusion criteria and were summarized in an evidence table. Thirteen studies published between 2006 and 2015 across seven countries showed a consistent increase in vitamin D lab tests and related costs. Many vitamin D testing patterns reflected screening rather than targeted testing for individuals at high risk of vitamin D deficiency or insufficiency. Interventions aimed at managing inappropriate clinical practices related to LVD were effective in the short term. Variability and controversy were pervasive in many aspects of vitamin D management, shining a light on physicians’ practices in the face of uncertainty. Future research is needed to inform better clinical guidelines and to assess implementation practices that encourage evidence-based management of LVD in adult populations.

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Section: Introductionmentioning

confidence: 99%

Clinical Management of Low Vitamin D: A Scoping Review of Physicians’ Practices

et al. 2018

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“…Increasing gradation of 25(OH)D 3 caused uorescence restoration in phosphate buffer as well as similar results was observed in even 50% serum. Even the assay utilizing VDBA 14 aptamer has been reported to have LOD of 1µM which is far away from 0.006 µM as reported by Anusha et al, 2020 8 . Improvement in this protocol by either increasing the speci city of the aptamer and reducing the uorescence constraints can help in development of chip model and used as point of care testing device.…”

Section: Resultsmentioning

confidence: 83%

Graphene Oxide and Fluorescent Aptamer Based Novel Biosensor for Detection of 25-hydroxyvitamin D3

Gupta

Kaul

Singh

et al. 2021

Preprint

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For maintaining the healthy metabolic status, vitamin D is a beneficial metabolite stored majorly in its pre-activated form, 25-hydroxyvitamin D3 (25(OH)D3). Due to its important role in bone strengthening, the study was planned to quantify 25(OH)D3 levels in our blood. Quantification techniques for 25(OH)D3 are costly thus requiring a need for a low cost, and sensitive detection methods. In this work, an economic, and sensitive sensor for the detection of 25(OH)D3 was developed using aptamer and graphene oxide (GO). Aptamer is an oligonucleotide, sensitive towards its target, whereas, GO with 2D nanosheets provides excellent quenching surface. Aptamer labeled with fluorescein (5’, 6-FAM) is adsorbed by π -π interaction on the GO sheets leading to quenching of the fluorescence due to Förster resonance energy transfer (FRET). However, in the presence of 25(OH)D3, a major portion of aptamer fluorescence remains unaltered, due to its association with 25(OH)D3. However, in the absence, aptamer fluorescence gets fully quenched. Fluorescence intensity quenching was monitored using fluorescence spectrophotometer and agarose gel based system. The limit of detection of 25(OH)D3 by this method was found to be 0.15 µg/mL. Therefore, this method could come up as a new sensing method in the field of vitamin D detection.

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“…Recently, a rapid immunoassay-based point-of-need diagnostic test for the assessment of 25(OH)D 3 in finger-stick blood was developed. The assay was accompanied by a smartphone-assisted imaging device which allows for easy operation and access to the results [32]. Moreover, a chemiluminescent immunoassay method was developed for determination of the sum of 1,25(OH) 2 D 2 and 1,25(OH) 2 D 3 .…”

Section: Immunoassay Techniquesmentioning

confidence: 99%

Clinical Significance of Analysis of Vitamin D Status in Various Diseases

et al. 2020

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Vitamin D plays a role not only in the proper functioning of the skeletal system and the calcium-phosphate equilibrium, but also in the immune system, the cardiovascular system and the growth and division of cells. Although numerous studies have reported on the analysis of vitamin D status in various groups of patients, the clinical significance of measurements of vitamin D forms and metabolites remains ambiguous. This article reviews the reports analyzing the status of vitamin D in various chronic states. Particular attention is given to factors affecting measurement of vitamin D forms and metabolites. Relevant papers published during recent years were identified by an extensive PubMed search using appropriate keywords. Measurement of vitamin D status proved to be a useful tool in diagnosis and progression of metabolic syndrome, neurological disorders and cancer. High performance liquid chromatography coupled with tandem mass spectrometry has become the preferred method for analyzing the various forms and metabolites of vitamin D in biological fluids. Factors influencing vitamin D concentration, including socio-demographic and biochemical factors as well as the genetic polymorphism of the vitamin D receptor, along with vitamin D transporters and enzymes participating in vitamin D metabolism should be considered as potential confounders of the interpretation of plasma total 25(OH)D concentrations.

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A Quantitative Point-of-Need Assay for the Assessment of Vitamin D3 Deficiency

Cited by 36 publications

References 26 publications

Clinical Management of Low Vitamin D: A Scoping Review of Physicians’ Practices

Clinical Management of Low Vitamin D: A Scoping Review of Physicians’ Practices

Graphene Oxide and Fluorescent Aptamer Based Novel Biosensor for Detection of 25-hydroxyvitamin D3

Clinical Significance of Analysis of Vitamin D Status in Various Diseases

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