Peroxidase production and ligninolytic potentials of fresh water bacteria Raoultella ornithinolytica and Ensifer adhaerens

Falade, Ayodeji O.; Eyisi, Onyedikachi A.L.; Mabinya, Leonard V.; Nwodo, Uchechukwu U.; Okoh, Anthony I.

doi:10.1016/j.btre.2017.10.001

Cited by 54 publications

(49 citation statements)

References 47 publications

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“…The organism was isolated from sediments of Tyhume River, Alice, South Africa, by an enrichment technique using kraft lignin (Sigma‐Aldrich, South Africa) as the sole carbon source . Subsequently, the isolate was carefully chosen on the basis of its ligninolytic potential . The 16S rDNA partial sequence analysis showed that the bacterial strain had 99% similarity to R. ornithinolytica strain G.W‐CD.10 .…”

Section: Methodsmentioning

confidence: 99%

“…Subsequently, the isolate was carefully chosen on the basis of its ligninolytic potential . The 16S rDNA partial sequence analysis showed that the bacterial strain had 99% similarity to R. ornithinolytica strain G.W‐CD.10 . Its nucleotide sequence is available in the GenBank of the National Centre for Biotechnology Information (NCBI) as Raoultella ornithinolytica strain OKOH‐1 under the accession number, KX640917.…”

Section: Methodsmentioning

confidence: 99%

“…Peroxidase was produced using the method of Falade et al. . The organism was cultivated in a kraft lignin modified fermentation medium (KLFM) of the following composition: 4.55 g/L K 2 HPO 4 , 0.53 g/L KH 2 PO 4 , 0.5 g/L MgSO 4 , 5 g/L NH 4 NO 3 , 0.1 g/L yeast extract, and 0.1% w/v kraft lignin.…”

Section: Methodsmentioning

confidence: 99%

“…Peroxidase activity was evaluated by the rate of hydrogen peroxide‐dependent oxidation of pyrogallol to purpurogallin using the modified method of Chance and Maehly described by Falade et al. . Briefly, the crude enzyme was added to the reaction mixture containing 5% w/v pyrogallol (substrate) and 0.1 mol L −1 potassium phosphate buffer (pH 6).…”

Section: Methodsmentioning

confidence: 99%

“…The emerging ligninolytic activity of proteobacteria confers desirable characteristics for exploitation in the search for novel peroxidase sources. Consequently, Raoultella ornithinolytica OKOH‐1, identified in previous study as a ligninolytic bacteria with an excellent potential for peroxidase production , was optimized in the valorization of agrowaste biomass for peroxidase production.…”

Section: Introductionmentioning

confidence: 99%

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Agrowastes utilization by Raoultella ornithinolytica for optimal extracellular peroxidase activity

Falade

Mabinya

Okoh

et al. 2018

Biotech and App Biochem

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The industrial applications and prospects of microbial peroxidase are on the upwards trend, thus necessitating the search for sources with high turnaround time. Actinobacterial species have been a major source of peroxidase for the obvious reasons of having robust metabolite expression capabilities. However, other bacteria species have been underexplored for peroxidase production, hence the motivation for the investigation into the peroxidase production potential of Raoultella ornithinolytica OKOH-1 (KX640917). The bacteria expressed optimum specific peroxidase activity of 16.48 ± 0.89 U mg −1 , which is higher than those previously reported. The optimal fermentation conditions were pH 5 (3.44 ± 0.64 U mL −1 ), incubation temperature of 35 • C (5.25 ± 0.00 U mL −1 ), and agitation speed of 150 rpm (9.45 ± 2.57 U mL −1 ), with guaiacol and ammonium chloride as the best inducer and nitrogen supplement, respectively. On valorization of agrowastes as a sole carbon source for the secretion of peroxidase, sawdust gave the best peroxidase yield (15.21 ± 2.48 U mg −1 ) under solid-state fermentation. Also, a nonperoxide-dependent enzyme activity, which suggests probable laccase activity, was observed. The ability of the bacteria to utilize agrowastes is highly economical and as well a suitable waste management strategy. Consequently, R. ornithinolytica OKOH-1 is a promising industrial strain with dexterity for enhanced peroxidase production.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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