Protein malnutrition after weaning disrupts peripheral clock and daily insulin secretion in mice

Borck, Patrícia Cristine; Batista, Thiago M.; Vettorazzi, Jean Franciesco; Camargo, Rafael Ludemann; Boschero, Antônio Carlos; Vieira, Elaine; Carneiro, Everardo M.

doi:10.1016/j.jnutbio.2017.08.013

Cited by 11 publications

(11 citation statements)

References 41 publications

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“…Dietary protein restriction induces several metabolic alterations including reduced body weight, increased energy expenditure and changes in the rhythmicity of glucose metabolism (Laeger et al 2014, Camargo et al 2015, Borck et al 2017. Here, we find that LP diet leads to lower body weight, despite a tendency of increased adiposity, which aligns with previous reports (Solon-Biet et al 2014, Camargo et al 2015, Branco et al 2019.…”

Section: Journal Of Endocrinologysupporting

confidence: 92%

“…Since these mice showed a similar pattern of energy intake and locomotor activity than mice fed an NP diet, we speculate that lower lean mass (Branco et al 2019) as well as increased energy expenditure (Laeger et al 2014, Solon-Biet et al 2014 contributes to reduced body weight in LP mice. Importantly, dietary protein restriction also leads to a persistent increase in insulin sensitivity at both periods analyzed, which further supports the action of LP diet in impacting the daily rhythms of glucose metabolism (Borck et al 2017).…”

Section: Journal Of Endocrinologysupporting

confidence: 61%

“…Indeed, mice fed to high-fat diet (HFD) and exposed to ALAN exhibited higher body and fat pads weights, lower energy expenditure and enhanced inflammatory responses in adipose tissue (Fonken et al 2013). Previous studies have demonstrated that low-protein (LP) diet also alters the circadian pattern of the peripheral clock, as well as the rhythmicity of glucose and lipid metabolism and the plasma insulin levels (Sutton et al 2010, Borck et al 2017). However, it remains unexplored whether dietary protein restriction could enhance the effects of exposure to ALAN on the energy metabolism predisposing to the development of metabolic diseases.…”

Section: Introductionmentioning

confidence: 99%

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Effect of nighttime light exposure on glucose metabolism in protein-restricted mice

Borck

Rickli

Vettorazzi

et al. 2022

Journal of Endocrinology

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Disruption of biological rhythms due exposure to artificial light at night (ALAN) has been emerged as new risk factor for metabolic diseases. However, it remains largely unexplored the effects induced by exposure to ALAN on energy metabolism with concomitant misalignment in the circadian system caused by nutritional imbalance. Objective: Here we evaluate whether low-protein diet could enhance the effects induced by exposure to ALAN on the energy metabolism and consequently predispose to metabolic disorders. Male C57BL6/J mice were weaned on a normal protein (NP) or a low-protein (LP) diet and housed on 12h light/dark (L/D) cycle. After 6 weeks, mice maintained on their respective diets were subdivided into normal light/dark cycle or exposed to ALAN for 8 weeks. We observed that exposure to ALAN concomitant to LP diet disrupts the behavioral rhythms, without shifting the timing of food intake. Furthermore, exposure to ALAN lead to increased body and fat pad weights, higher levels of fast and fed glycemia and glucose intolerance independent of the diet consumed. Importantly the insulin resistance developed in mice exposed to ALAN was diet-dependent. At the molecular level, exposure to ALAN concurrent with LP diet increased the expression of Phosphoenolpyruvate carboxykinase 1 in both periods analyzed and inverted the pattern of Fibroblast growth factor 21 (Fgf21) expression in the liver. Our data suggest that dietary protein restriction modulates the effects induced by nighttime light exposure on glucose metabolism, which could be partially related with the dysregulation on hepatic Fgf21 expression.

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Section: Journal Of Endocrinologysupporting

confidence: 92%

Section: Journal Of Endocrinologysupporting

confidence: 61%

Section: Introductionmentioning

confidence: 99%

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Effect of nighttime light exposure on glucose metabolism in protein-restricted mice

Borck

Rickli

Vettorazzi

et al. 2022

Journal of Endocrinology

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“…Blood samples were collected in heparinized tubes, and centrifuged at 3000 g to obtain plasma, which was stored at −20°C. Plasma glucose and triglyceride levels were measured using the DiaSys kit (cat: 1 2531 99 90 314 and cat: 1 5760 99 90 314, respectively) in a Selectra Junior auto analyzer (Vital Scientific, Rheden, Gelderland, Netherlands), and insulin was measured by radioimmunoassay, as previously reported . In addition, retroperitoneal fat pads were collected and weighed to assess the obesity induced by MSG treatment.…”

Section: Methodsmentioning

confidence: 99%

“…1 5760 99 90 314, respectively) in a Selectra Junior auto analyzer (Vital Scientific, Rheden, Gelderland, Netherlands), and insulin was measured by radioimmunoassay, as previously reported. 49 In addition, retroperitoneal fat pads were collected and weighed to assess the obesity induced by MSG treatment.…”

Section: Body Weight and Biochemical Plasma Parametersmentioning

confidence: 99%

Swimming training reduces glucose‐amplifying pathway and cholinergic responses in islets from lean‐ and MSG‐obese rats

Borck

Leite

Valcanaia

et al. 2019

Clin Exp Pharma Physio

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Here, we investigate the effects of exercise training on glucose‐ and cholinergic‐induced insulin secretion in pancreatic islets from obese and lean rats. Male Wistar rats were treated with monosodium glutamate (MSG) for the first 5 days of life, while control (CON) rats received saline. At 21 days, the rats were divided into exercised (EXE) and sedentary (SED) groups. The EXE rats swam for 30 minutes, three times/week, for 10 weeks. After this, MSG‐SED rats showed hyperglycaemia, hypertriglyceridaemia and hyperinsulinaemia. Besides, islets from MSG‐SED rats exhibited increased glucose‐stimulated insulin secretion (GSIS), followed by impaired glucose sensitivity, absence of glucose‐amplifying pathway and weak cholinergic response. In contrast, adiposity, hyperinsulinaemia and hypertriglyceridaemia were reduced in MSG‐EXE rats. Moreover, islets from MSG‐EXE rats exhibited lower GSIS and improved islet glucose sensitivity, without restoration of the glucose‐amplifying pathway or alteration in the weak cholinergic effect of these islets. In islets from CON‐EXE rats we also observed reduced GSIS and absence of glucose‐amplifying effects and an accentuated reduction in cholinergic insulinotropic responses, without effect on glucose sensitivity in pancreatic islets from this group. Neither obesity nor exercise modified Muscarinic Receptor 3 (M3R) immunocontent or its downstream pathways (PKC and PKA). Moreover, only CON‐EXE showed increased GSIS in the presence of calcium blocker, Thapsigargin. In conclusion, swimming training reduces GSIS and cholinergic responsiveness in isolated pancreatic islets from lean and hypothalamic obese rats, which could be due to the inhibition of glucose‐amplifying pathways.

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Association between protein undernutrition and diabetes: Molecular implications in the reduction of insulin secretion

dos Reis Araujo,

Alves,

dos Santos

et al. 2023

Rev Endocr Metab Disord

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Protein malnutrition after weaning disrupts peripheral clock and daily insulin secretion in mice

Cited by 11 publications

References 41 publications

Effect of nighttime light exposure on glucose metabolism in protein-restricted mice

Effect of nighttime light exposure on glucose metabolism in protein-restricted mice

Swimming training reduces glucose‐amplifying pathway and cholinergic responses in islets from lean‐ and MSG‐obese rats

Association between protein undernutrition and diabetes: Molecular implications in the reduction of insulin secretion

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