Liquid chromatography tandem mass spectrometric determination of triterpenes in human fluids: Evaluation of markers of dietary intake of olive oil and metabolic disposition of oleanolic acid and maslinic acid in humans

Pozo, Óscar J.; Pujadas, Mitona; Gleeson, Sarah Biel; Mesa, María Dolores; Pastor, Antoni; Kotronoulas, Aristotelis; Fitó, Montserrat; Covas, Marı́a-Isabel; Navarro, José Ramón Fernández; Espejo, J. A.; Sánchez-Rodriguez, Estefanía; Marchal, Rosa; Calleja, Miguel Ángel; Torre, Rafael de la

doi:10.1016/j.aca.2017.07.041

Cited by 21 publications

(11 citation statements)

References 20 publications

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“…Despite the limitations, to our knowledge, this is the first RCT to demonstrate the beneficial effects, in aged, community-dwelling individuals, of combined the MA supplementation and moderate RT on upper body muscle mass, and the parameters of mobility functions, such as grip strength and knee pain score. Further studies are warranted to confirm the present findings using the metabolic disposition of MA in plasma and urine samples (42,43) and to elucidate the effect of MA alone on mobility function in older population.…”

Section: Discussionsupporting

confidence: 54%

Maslinic acid derived from olive fruit in combination with resistance training improves muscle mass and mobility functions in the elderly

Nagai

Yagyu

Hata

et al. 2019

J. Clin. Biochem. Nutr.

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Maslinic acid, derived from olive fruit, reduces pro inflammation cytokines, which are involved in muscle fiber atrophy. Therefore, the maslinic acid ingestion may enhance the muscular response to resistance training through anti inflammatory action. We therefore conducted a parallel, double blind, randomized, placebo controlled trial that examined whether a combination of maslinic acid supplementation and resistance training improve mobility func tions in community dwelling elderly persons. Over a 12 week period, 36 participants underwent moderate resistance training and are assigned to the maslinic acid supplementation (n = 17, 60 mg/day) or the placebo (n = 19) group. At baseline and at 12 weeks, we assessed body composition, grip strength, walking speed, leg strength, mobility functions, and knee pain scores. Following the 12 weeks, skeletal muscle mass, segmental muscle mass (right arm, left arm, and trunk) and knee pain score of the right leg were significantly improved in the maslinic acid group, while there was no change or parameters had worsened in the placebo group. Grip strength of the better side significantly increased only in the maslinic acid group. These results suggest that maslinic acid supplementation combined with moderate resistance training may increase upper muscle mass and grip strength, and reduce knee pain, could be effective for preventing mobility related disability in elderly persons. Clinical trial registra tion number: UMIN000017207.

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Section: Discussionsupporting

confidence: 54%

Maslinic acid derived from olive fruit in combination with resistance training improves muscle mass and mobility functions in the elderly

Nagai

Yagyu

Hata

et al. 2019

J. Clin. Biochem. Nutr.

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“…To ensure the subjects’ compliance with the assigned intervention, triterpenes (maslinic and oleanolic acid) derivatized with 2-picolylamine (see Appendix A ) and olive oil phenolic compounds (hydroxytyrosol and metabolites) were analyzed in 24-h urine from 12 random subjects by liquid chromatography coupled to a mass spectrometer [ 22 ].…”

Section: Methodsmentioning

confidence: 99%

Effects of Virgin Olive Oils Differing in Their Bioactive Compound Contents on Metabolic Syndrome and Endothelial Functional Risk Biomarkers in Healthy Adults: A Randomized Double-Blind Controlled Trial

et al. 2018

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The aim of this study was to evaluate the effect of virgin olive oils (VOOs) enriched with phenolic compounds and triterpenes on metabolic syndrome and endothelial function biomarkers in healthy adults. The trial was a three-week randomized, crossover, controlled, double-blind, intervention study involving 58 subjects supplemented with a daily dose (30 mL) of three oils: (1) a VOO (124 ppm of phenolic compounds and 86 ppm of triterpenes); (2) an optimized VOO (OVOO) (490 ppm of phenolic compounds and 86 ppm of triterpenes); and (3) a functional olive oil (FOO) high in phenolic compounds (487 ppm) and enriched with triterpenes (389 ppm). Metabolic syndrome and endothelial function biomarkers were determined in vivo and ex vivo. Plasma high density lipoprotein cholesterol (HDLc) increased after the OVOO intake. Plasma endothelin-1 levels decreased after the intake of the three olive oils, and in blood cell cultures challenged. Daily intake of VOO enriched in phenolic compounds improved plasma HDLc, although no differences were found at the end of the three interventions, while VOO with at least 124 ppm of phenolic compounds, regardless of the triterpenes content improved the systemic endothelin-1 levels in vivo and ex vivo. No effect of triterpenes was observed after three weeks of interventions. Results need to be confirmed in subjects with metabolic syndrome and impaired endothelial function (Clinical Trials number NCT02520739).

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“…Previous work performed by our research group demonstrated the usefulness of 2-PA for the determination of monocarboxylic acids in complex matrices such as urine [21,22] although its suitability for derivatization of polycarboxylic acids in complex matrices remains unexplored. On the other hand, the usefulness of oBHA for the derivatization of polycarboxylic acids has been demonstrated [24,25] although its suitability for the determination of these metabolites in complex matrices has not been proved.…”

Section: Introductionmentioning

confidence: 99%

“…A broad variety of derivatizing agents are used in LC-MS/MS. For instance, Girard's reagents and hydroxylamine are used for derivatizing ketones [16,17], dansyl chloride is used for aliphatic alcohols [18] phenols and amines [19], 2-picolyl amine (2-PA) and N-methyl-2phenylethanamine are used for carboxylic acids [20][21][22][23] and o-benzylhydroxyl amine (oBHA) [24,25] is used for the derivatization of both ketones and carboxylic acids.…”

Section: Introductionmentioning

confidence: 99%

Improving liquid chromatography-tandem mass spectrometry determination of polycarboxylic acids in human urine by chemical derivatization. Comparison of o-benzyl hydroxylamine and 2-picolyl amine

Gómez-Gómez

Soldevila

Pizarro

et al. 2019

Journal of Pharmaceutical and Biomedical Analysis

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Liquid chromatography tandem mass spectrometric determination of triterpenes in human fluids: Evaluation of markers of dietary intake of olive oil and metabolic disposition of oleanolic acid and maslinic acid in humans

Cited by 21 publications

References 20 publications

Maslinic acid derived from olive fruit in combination with resistance training improves muscle mass and mobility functions in the elderly

Maslinic acid derived from olive fruit in combination with resistance training improves muscle mass and mobility functions in the elderly

Effects of Virgin Olive Oils Differing in Their Bioactive Compound Contents on Metabolic Syndrome and Endothelial Functional Risk Biomarkers in Healthy Adults: A Randomized Double-Blind Controlled Trial

Improving liquid chromatography-tandem mass spectrometry determination of polycarboxylic acids in human urine by chemical derivatization. Comparison of o-benzyl hydroxylamine and 2-picolyl amine

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