Structural analysis of an innate immunostimulant from broccoli, &lt;i&gt;Brassica oleracea&lt;/i&gt; var. &lt;i&gt;italica &lt;/i&gt;

Urai, Makoto; Kataoka, Keiko; Nishida, Satoshi; Sekimizu, Kazuhisa

doi:10.5582/ddt.2017.01044

Cited by 7 publications

(15 citation statements)

References 20 publications

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“…Pectic fractions isolated by sequential extraction from broccoli stem had arabinose, not galactose, as the main neutral monosaccharide (Houben et al, 2011). A pectin with immunostimulatory properties purified from an aqueous extract of edible parts of broccoli also had galacturonic acid, arabinose, galactose, rhamnose and glucose in a molar ratio of 12: 7.3: 4.9: 1.2: 1.0 (Urai et al, 2017). The apparent discrepancy is probably due to the hydrolysis of the more labile linkages with arabinose in the acidic extraction conditions (Thibault, Renard, Axelos, Roger, & Crépeau, 1993).…”

Section: Extraction and Chemical Composition Of Pectinmentioning

confidence: 99%

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Pectins from food waste: Characterization and functional properties of a pectin extracted from broccoli stalk

Petkowicz

Williams

2020

Food Hydrocolloids

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Section: Extraction and Chemical Composition Of Pectinmentioning

confidence: 99%

“…More recently, Urai, Kataoka, Nishida, & Sekimizu (2017) isolated a pectic fraction from an aqueous extract obtained from edible parts of broccoli. However, we are not aware of any studies concerning the acid extraction and characterization of pectins from broccoli stalk.…”

Section: Introductionmentioning

confidence: 99%

Pectins from food waste: Characterization and functional properties of a pectin extracted from broccoli stalk

Petkowicz

Williams

2020

Food Hydrocolloids

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“…Muscle specimens were prepared by rearing 5 th instar silkworms on artificial diet for 5 days since the last ecdysis (10). The sample solution (50 μL) was injected into the muscle specimens using a tuberculin syringe (1mL) with a 27-gauge needle, and the body length was monitored for 10 min.…”

Section: Methodsmentioning

confidence: 99%

“…In our previous study, we found that the hot water extract of the edible part of broccoli showed higher specific activity in the muscle contractile system of the silkworm than other vegetables (10). In addition, the active component was ethanol precipitated, subjected to diethylaminoethyl (DEAE)-cellulose column chromatography, and purified by eluting the DEAE-bound fraction, revealing its structure to be a pectin-like acidic polysaccharide (10). On the other hand, some neutral polysaccharides in different plant materials have recently been reported to have physiological effects on the innate immune system (11–14), but there are no such reports for broccoli.…”

Section: Introductionmentioning

confidence: 99%

Activation of innate immunity by neutral polysaccharides from broccoli buds

Miyashita

Kataoka

Sekimizu

2021

Preprint

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Edible substances that stimulate the innate immune system are good candidates for functional foods to improve human health. We have previously reported that acidic polysaccharides from broccoli extract exhibit immunostimulatory effects, but neutral polysaccharides have been overlooked. In the present study, we found that neutral polysaccharides have significantly stronger (higher specific activity) immunostimulatory activity than acidic polysaccharides. The hot water extract of broccoli showed the immunostimulatory activity in the silkworm muscle contraction assay, suggesting that it stimulates innate immunity via paralytic peptide pathway. The activity was concentrated in the buds, but not in the stems and stalk. The active substance was recovered in the flow-through fraction of diethylaminoethyl-cellulose column chromatography with neutral polysaccharides. The specific activity of the fraction was significantly higher than that of the acidic polysaccharides from broccoli reported previously. These results suggest that the neutral polysaccharide present in broccoli buds stimulates innate immunity and can be semi-purified by one-step chromatography.

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“…Of the broccoli polysaccharides with immunostimulatory activity, acidic polysaccharides have been characterized to be pectin-like polysaccharides that precipitate in ethanol and bind to diethylamino-ethyl (DEAE) cellulose column (10). However, for neutral polysaccharides that do not bind to DEAE cellulose columns, there is virtually no information on their chemical structures.…”

Section: Introductionmentioning

confidence: 99%

Purification of immunostimulatory glucose homopolymer from broccoli (Brassica oleracea var. italica)

Miyashita

Kataoka

Tsuchida

et al. 2021

Preprint

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We prepared broccoli (Brassica oleracea var. italica) neutral polysaccharides (flow-through fractions of anion exchange column chromatography from hot water extracts) from different broccoli cultivars and compared their immunostimulatory effects in the silkworm muscle contraction assay. The cultivars showed a wide range of activity, with the cultivar 'Winter dome' showing the highest specific activity (more than 100 times higher than curdlan). Furthermore, the active substance was purified by gel filtration column chromatography. The active substance showed heterogeneous molecular weights of more than 270 kDa. Sugar composition analysis of the purified fraction revealed that more than 95% of its sugar component was glucose, suggesting that the immunostimulatory neutral polysaccharide from broccoli cultivar Winter dome was a homopolymer of glucose. The purified fraction also induced TNF production in cultured mouse macrophage cells. These results suggest that the glucose homopolymer in broccoli has an immunostimulatory effect on both arthropod and mammalian immune system.

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Structural analysis of an innate immunostimulant from broccoli, <i>Brassica oleracea</i> var. <i>italica </i>

Cited by 7 publications

References 20 publications

Pectins from food waste: Characterization and functional properties of a pectin extracted from broccoli stalk

Pectins from food waste: Characterization and functional properties of a pectin extracted from broccoli stalk

Activation of innate immunity by neutral polysaccharides from broccoli buds

Purification of immunostimulatory glucose homopolymer from broccoli (Brassica oleracea var. italica)

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