Viral Surveillance in Serum Samples From Patients With Acute Liver Failure By Metagenomic Next-Generation Sequencing

Somasekar, Sneha; Lee, Deanna; Rule, Jody; Naccache, Samia N.; Stone, Mars; Busch, Michael P.; Sanders, Corron; Lee, William M.; Chiu, Charles Y.

doi:10.1093/cid/cix596

Cited by 84 publications

(72 citation statements)

References 41 publications

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“…To the best of our knowledge, this is the first study to investigate pathogen(s) of pediatric and adult myocarditis using NGS. An NGS‐based approach enables us to identify pathogenic microbes comprehensively and to detect virus more simply than isolation, and stored specimens can be used . Recently, NGS has been applied in the field of infectious diseases, and its utility has been indicated .…”

Section: Discussionmentioning

confidence: 99%

“…Recently, next‐generation sequencing (NGS) has enabled the comprehensive detection of pathogenic microorganisms, and this method has been applied in the field of infectious diseases . We previously analyzed sera of encephalitis, fulminant hepatitis, and bloodstream infection with unknown etiology and identified viral and bacterial genomes of presumptive pathogens of the diseases .…”

Section: Introductionmentioning

confidence: 99%

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Identification of potential pathogenic viruses in patients with acute myocarditis using next‐generation sequencing

Takeuchi

Kawada

Horiba

et al. 2018

Journal of Medical Virology

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Myocarditis is an inflammatory disease of the myocardium and leads to cardiac dysfunction and heart failure. Although viral infections are considered to be the most common etiology of myocarditis, the identification of the causative virus is still challenging. Recently, next-generation sequencing (NGS) has been applied in the diagnosis of infectious diseases. The aim of the current study was to comprehensively analyze potential pathogenic microorganisms using NGS in the sera of patients with myocarditis. Twelve pediatric and five adult patients hospitalized for acute myocarditis were included. Serum samples in the acute phase were obtained and analyzed using NGS to detect pathogen-derived DNA and RNA. Viral sequence reads were detected in 7 (41%) of the 17 myocarditis patients by NGS. Among these patients, detection of Epstein-Barr virus, human parvovirus B19, torque teno virus, and respiratory syncytial virus reads by NGS was consistent with polymerase chain reaction or antigen test results in one patient each. A large number of human pegivirus reads were detected from one patient by RNA sequencing; however, its pathogenicity to human is unknown. Conversely, the number of detected virus-derived reads was small in most cases, and the pathophysiological role of these viruses remains to be clarified. No significant bacterial or fungal reads other than normal bacterial flora was detected. These data indicate that comprehensive detection of virus-derived DNA and RNA using NGS can be useful for the identification of potential pathogenic viruses in myocarditis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification of potential pathogenic viruses in patients with acute myocarditis using next‐generation sequencing

Takeuchi

Kawada

Horiba

et al. 2018

Journal of Medical Virology

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“…Acute liver failure (ALF) is a devastating but rare illness affecting 2,000 individuals in the United States yearly . Herpes simplex virus (HSV) is known to cause ALF rarely, but the extent of its reach is unclear because it can be difficult to diagnose . We set out to identify and characterize cases of HSV‐related ALF within the Acute Liver Failure Study Group (ALFSG), a network that has prospectively collected more than 2,600 cases of ALF over the past 21 years.…”

Section: Characteristics Of Hsv‐related Alf Versus All Other Virusesmentioning

confidence: 99%

Herpes Simplex Virus–Associated Acute Liver Failure Often Goes Unrecognized

Little

Rule

Peng³

et al. 2019

Hepatology

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Acute liver failure (ALF) is a devastating but rare illness affecting 2000 individuals in the U.S. yearly. Herpes simplex virus (HSV) is known to cause ALF rarely, but the extent of its reach is unclear since it can be difficult to diagnose. We set out to identify and characterize cases of HSV-related ALF within the Acute Liver Failure Study Group, (ALFSG), a network that has prospectively collected more than 2,600 cases of ALF over the past 21 years. This article is protected by copyright. All rights reserved.

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“…Within this group, they identified eight cases of previously unrecognised viral infections. These included four cases of HSV‐1 (with one hepatitis B (HBV) co‐infection), in addition to single instances of hepatitis B virus, parvovirus B19, human herpes virus‐7 (HHV‐7) and cytomegalovirus …”

Section: Methodsmentioning

confidence: 99%

Systematic review: non A‐E, seronegative or indeterminate hepatitis; what is this deadly disease?

Brennan

Donnelly

Simpson

2018

Aliment Pharmacol Ther

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Whilst various pathological processes have been implicated in the development of indeterminate hepatitis, the specific cause remains elusive. There is an urgent need for general consensus on a specific definition and exclusion of confounding aetiologies with coordinated multicentre investigation of this rare condition to identify aetiology and develop therapies to reduce the significant mortality and need for emergency liver transplantation associated with this condition.

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Viral Surveillance in Serum Samples From Patients With Acute Liver Failure By Metagenomic Next-Generation Sequencing

Abstract: Viral metagenomic surveillance of 187 patients with indeterminate acute liver failure (ALF) from 1998 to 2010 suggests that these patients should be screened for the presence of uncommon viruses and coinfections, although novel viruses associated with ALF were not identified.

Cited by 84 publications

References 41 publications

Identification of potential pathogenic viruses in patients with acute myocarditis using next‐generation sequencing

Identification of potential pathogenic viruses in patients with acute myocarditis using next‐generation sequencing

Herpes Simplex Virus–Associated Acute Liver Failure Often Goes Unrecognized

Systematic review: non A‐E, seronegative or indeterminate hepatitis; what is this deadly disease?

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