A matrix-assisted laser desorption/ionization time of flight mass spectrometry reference database for the identification of Histoplasma capsulatum

Valero, Clara; Buitrago, María J.; Gago, Sara; Quiles-Melero, Inmaculada; García-Rodríguez, Julio

doi:10.1093/mmy/myx047

Cited by 30 publications

(21 citation statements)

References 32 publications

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“…64 A matrix-assisted laser desorption/ ionization time of flight mass spectrometry (MALDI-TOF MS) reference database for the identification of H. capsulatum has been developed; MALDI-TOF MS could reduce time to identification and also decrease risk to laboratory personnel cultivating the fungus. 65…”

Section: Molecular and Other Diagnosticsmentioning

confidence: 99%

Current Concepts in the Epidemiology, Diagnosis, and Management of Histoplasmosis Syndromes

Azar

Loyd

Relich

et al. 2020

Semin Respir Crit Care Med

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Histoplasmosis is a global disease endemic to regions of all six inhabited continents. The areas of highest endemicity lie within the Mississippi and Ohio River Valleys of North America and parts of Central and South America. As a result of climate change and anthropogenic land utilization, the conditions suitable for Histoplasma capsulatum are changing, leading to a corresponding change in epidemiology. The clinical manifestations of histoplasmosis are protean, variably resembling other common conditions such as community-acquired pneumonia, tuberculosis, sarcoidosis, Crohn's disease, or malignancy. Making a successful diagnosis is contingent on a thorough understanding of epidemiology, common clinical presentations, and best testing practices for histoplasmosis. While most subclinical or self-limited diseases do not require treatment in immunocompetent patients, all immunocompromised patients and those with progressive disseminated disease or chronic pulmonary disease should be treated. Liposomal amphotericin B is the preferred agent for severe or disseminated disease, while itraconazole is adequate for milder cases and “step-down” therapy following response to amphotericin B. In this review, we discuss the current evidence-based approaches to the epidemiology, diagnosis, and management of histoplasmosis.

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Section: Molecular and Other Diagnosticsmentioning

confidence: 99%

Current Concepts in the Epidemiology, Diagnosis, and Management of Histoplasmosis Syndromes

Azar

Loyd

Relich

et al. 2020

Semin Respir Crit Care Med

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show abstract

“…Isolation of the fungus in culture is considered the gold standard for diagnosis. H. capsulatum can grow in mycological media of common use in routine laboratories, and current MALDI-TOF systems can provide a presumptive identification (Panda et al, 2015;Rizzato et al, 2015;Rychert et al, 2018;Valero et al, 2018). Cultures, however, have well-known limitations, i.e., slowness, suboptimal sensitivity, and requirement of BSL3 facilities to manipulate them (Kauffman, 2009).…”

Section: Handicaps Of Laboratory Diagnosis In Non-endemic Areasmentioning

confidence: 99%

Timely Diagnosis of Histoplasmosis in Non-endemic Countries: A Laboratory Challenge

Buitrago

Martín-Gómez

2020

Front. Microbiol.

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Human histoplasmosis is a fungal infection caused by the inhalation of microconidia of the thermally dimorphic fungi Histoplasma capsulatum. Autochthonous cases of histoplasmosis have been diagnosed in almost every country, but it is considered an endemic infection in specific areas of the world. Many of them are popular travel destinations or the source of migratory movements. Thus, the vast majority of the registered cases in non-endemic countries are imported. They correspond to people having been exposed to the fungus in endemic locations as immigrants, expatriates, transient workers or tourists, with reported cases also associated to organ donation. Misdiagnosis and delays in initiation of treatment are not uncommon in cases of imported histoplasmosis. They are associated to high fatality-rates specially in patients with compromised cellular immunity in which progressive disseminated forms develop. The diagnosis of this infection in non-endemic countries is hampered by the lack of clinical suspicion and a dearth of available diagnostic tools adequate to offer rapid and accurate results. Non-culture-based assays such as nucleic-acid amplification tests present as a suitable alternative in this situation, offering improved sensitivity and specificity, shortened turnaround time, and increased biosafety by avoiding culture manipulation. In non-endemic regions, molecular techniques are being used mainly in laboratories from countries that have registered an increase in the incidence of imported cases. However, the number of published techniques is limited and lack consensus. Efforts are currently under way to standardize nucleic acid amplificationbased techniques for its implementation in areas registering a rising number of imported cases.

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“…Alternatively, single-stranded DNA probes could also be used to detect the fungus in cultures (AccuProbe, Hologic, CA, USA), however the procedure is technically complex and time consuming [16]. In last years, matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-ToF MS) has started to be developed for the identification of H. capsulatum strains [17][18][19]. Although reports are still scarce, results in this area are promising since the system is able for recognizing different varieties (var.…”

Section: Culturementioning

confidence: 99%

“…capsulatum, var. duboisii) and forms of the pathogen (yeast and mold), thus reducing response time and decreasing the risk of culture manipulation [19].…”

Section: Culturementioning

confidence: 99%

Laboratory Diagnosis of Histoplasmosis: An Update

Buitrago¹,

Valero²

2020

Histoplasma and Histoplasmosis

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Early diagnosis of histoplasmosis is essential to establish a suitable antifungal therapy and reduce morbidity and mortality rates. However, laboratory diagnosis remains challenging due to the low availability of proper methods and the lack of clinical suspicion. Conventional diagnosis is still largely used even though limitations are well known. Isolating the fungus is time consuming and requires manipulation in BSL3 facilities, while direct visualization and histopathology techniques show low sensitivity and need skilled personnel. New approaches based on the detection of antibodies and antigens have been developed and commercialized last years. Although sensitivity and specificity of these methods is variable, antigen detection has been recently listed as an essential diagnostic test for AIDS patients due to its excellent performance. DNA detection methods are recognized as promising tools but there is still a lack of consensus among laboratories and there are not commercial tests available. Not all methods are widely available, thus most laboratories combine classical and other tests in order to overcome aforementioned limitations. In this chapter, we review the diagnostic pipeline currently available for the diagnosis of histoplasmosis in microbiological laboratories, from conventional to new developed tests. Most recent approaches are introduced and future perspectives are discussed.

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A matrix-assisted laser desorption/ionization time of flight mass spectrometry reference database for the identification of Histoplasma capsulatum

Cited by 30 publications

References 32 publications

Current Concepts in the Epidemiology, Diagnosis, and Management of Histoplasmosis Syndromes

Current Concepts in the Epidemiology, Diagnosis, and Management of Histoplasmosis Syndromes

Timely Diagnosis of Histoplasmosis in Non-endemic Countries: A Laboratory Challenge

Laboratory Diagnosis of Histoplasmosis: An Update

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